FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_E1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC50.30413625304136255No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC40.24330900243309003No Hit
GGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGG30.18248175182481752No Hit
GTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGA20.12165450121654502No Hit
GCCAGACAGCGACCAAAAGAATTCGGATGGCCACCAATCTGTCTCTTATA20.12165450121654502No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG20.12165450121654502No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG20.12165450121654502No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACCTGTCTCTTATACAC20.12165450121654502No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCCT20.12165450121654502No Hit
ATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGCTGTCTCTTATACACATC20.12165450121654502No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA20.12165450121654502No Hit
AGGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA20.12165450121654502No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA20.12165450121654502No Hit
TCCTCAGGTAGTGGTCCATCAATCGGGTTGAGCTGGGGTGCACCAGTCTC20.12165450121654502No Hit
ATCTCGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTC20.12165450121654502No Hit
TTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGAC20.12165450121654502No Hit
GGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA20.12165450121654502No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGCTGTCTCTTA20.12165450121654502No Hit
AGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACA20.12165450121654502No Hit
ATATGAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCCTGTCTCTT20.12165450121654502No Hit
GATTTTGGCGATCTATTCAACTGTCGCCAGTTCATTGGTACTGGTAGTCT20.12165450121654502No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT20.12165450121654502No Hit
CTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAGGAATGGG20.12165450121654502No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT20.12165450121654502No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT20.12165450121654502No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGCTGTCTCTTATA20.12165450121654502No Hit
GGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAA20.12165450121654502No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCA20.12165450121654502No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT20.12165450121654502No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTCTGTCTCTT20.12165450121654502No Hit
GGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGG20.12165450121654502No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC20.12165450121654502No Hit
GCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATT20.12165450121654502No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.12165450121654502No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT20.12165450121654502No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT20.12165450121654502No Hit
CTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAACCTGTCTCTT20.12165450121654502No Hit
GCTGATTCACAGCATCGGTCTCACAGACAGATGGCTACTACCACCAATCC20.12165450121654502No Hit
ATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAA20.12165450121654502No Hit
GTTTTGTTCAGCATCCAGAACTAACAGGACTGGATCTGTCTCTTATACAC20.12165450121654502No Hit
GTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGAT20.12165450121654502No Hit
GGGCCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTG20.12165450121654502No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT20.12165450121654502No Hit
ATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCA20.12165450121654502No Hit
GAAAATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACC20.12165450121654502No Hit
CAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTA20.12165450121654502No Hit
CCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTGTC20.12165450121654502No Hit
GTCTACCTGAAAGCTTGACATGGTGTTGGAGCTGTCTCTTATACACATCT20.12165450121654502No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCCATATCTGTCTCTTATACAC20.12165450121654502No Hit
TTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTT20.12165450121654502No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC20.12165450121654502No Hit
ATCCATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGCTGTCT20.12165450121654502No Hit
AATCCATTGTCTGCAAATCGCTTGCGGATATGCCAAAGGAAACAGTCTAC20.12165450121654502No Hit
GTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTG20.12165450121654502No Hit
CTGTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCAC20.12165450121654502No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT20.12165450121654502No Hit
CTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGATGGTGATAAC20.12165450121654502No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAG20.12165450121654502No Hit
ATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGATCTTG20.12165450121654502No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20.12165450121654502No Hit
ACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAA20.12165450121654502No Hit
GAACAAAATCACTCATGTCAGATGAAGTCCCATGTTCAAAGGCGCGCAAA20.12165450121654502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)