FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4082
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG150.3674669279764821No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT130.3184713375796179No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT100.2449779519843214No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA100.2449779519843214No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA90.22048015678588928No Hit
GTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAATGTGCAATA90.22048015678588928No Hit
GGGTTGGTTATTGACTGAAACTGGGGATGAGGTAATGCTCTTTGTTGACT90.22048015678588928No Hit
GAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAAATCAGC80.19598236158745713No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC80.19598236158745713No Hit
GTACAAATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGA80.19598236158745713No Hit
GTCTTAGCCAAGTCACTTCACCACTTTTTGACTGTTTACTCATTTGCCAA80.19598236158745713No Hit
GTGCTGATTTGTACCATTATCAATTAATTTGGCCCATTCACAAACATAAC80.19598236158745713No Hit
AACTAACCCCTTTTAGCTTTAGTAGGCTCATCTGTGAAATGGAGATGATG80.19598236158745713No Hit
ATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAA80.19598236158745713No Hit
GTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAATTAATT80.19598236158745713No Hit
GTTGAATATATTGTGGACCTCTAGGTCGACAGCCCTGAAGCTATTTTAAA70.17148456638902498No Hit
GTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGG70.17148456638902498No Hit
TTATATAACAATGCATGGCAAATTTCAAAGGACTGAAATCACGCACAGAG70.17148456638902498No Hit
GAATAGAACCGAGTCCAGAAATCAACCCACACAAGGACCGCCAGCGGATT70.17148456638902498No Hit
AATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAA70.17148456638902498No Hit
GCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGC60.14698677119059284No Hit
TATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTAACTAACCCCT60.14698677119059284No Hit
ACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCAGATCA60.14698677119059284No Hit
ATTCTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGC60.14698677119059284No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA60.14698677119059284No Hit
TGCTTAATGATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATG60.14698677119059284No Hit
GTGTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAG60.14698677119059284No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA60.14698677119059284No Hit
AATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGACTGAA60.14698677119059284No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC60.14698677119059284No Hit
GGCAAGAAAATACCACAATAATTGGTGTCCTTTGTTAAGCGCTATTATGT60.14698677119059284No Hit
TCCTAACCATACACAGGCAGATGAGTATTTACATGTGTAATCACCTTCCA60.14698677119059284No Hit
GGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTT60.14698677119059284No Hit
CTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACC60.14698677119059284No Hit
GTCTTAAGAATATGCTGTTAAGAGAACAAGATAAAGGGTGAGTGGGAGAG60.14698677119059284No Hit
CTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGCCTG60.14698677119059284No Hit
CCCATTTCCTTTGCTTGAACTCTCTGGCCTTTTCTCATATGCATTTGACA60.14698677119059284No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA50.1224889759921607No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT50.1224889759921607No Hit
CTTTTTTATGATAGAGGCACTGAAACTAAAGAAAATGGTGGAAGAAAGTT50.1224889759921607No Hit
ATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTA50.1224889759921607No Hit
GTGATTACACATGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCA50.1224889759921607No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT50.1224889759921607No Hit
CCTTCCAGATCATGATATAGAATACTTAAGTCAACAAAGAGCATTACCTC50.1224889759921607No Hit
ATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAA50.1224889759921607No Hit
GTATTAGGGAACAGAACGAGTTACCAGGAAGCTCCCATACGGAATTTAGG50.1224889759921607No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAGAAGCAGGCTTCTCACGAACA50.1224889759921607No Hit
AACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTA50.1224889759921607No Hit
ATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGCATTTTATTAAG50.1224889759921607No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG50.1224889759921607No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT50.1224889759921607No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA50.1224889759921607No Hit
GTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTT50.1224889759921607No Hit
GCACTGGAGGAACGCAACAATGCATTTTATTAAGGAAATTCCAGTCTGTG50.1224889759921607No Hit
GTTATTGACTGAAACTGGGGATGAGGTAATGCTCTTTGTTGACTTAAGTA50.1224889759921607No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC50.1224889759921607No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG50.1224889759921607No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG50.1224889759921607No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA50.1224889759921607No Hit
AGGAACGGGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCC50.1224889759921607No Hit
ACTTAGAAGTTTGTTGGTCAGTTTCTAAATACTTGGAGGGTTTTCTAAGT50.1224889759921607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)