FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6008
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC210.34953395472703064No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC180.2996005326231691No Hit
TATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTAACTAACCCCT160.2663115845539281No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA160.2663115845539281No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT160.2663115845539281No Hit
GTACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCAT140.2330226364846871No Hit
GGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAG140.2330226364846871No Hit
GAATTACTTTACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGC140.2330226364846871No Hit
TACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTT130.21637816245006655No Hit
CAATAATTGGTGTCCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTAT120.1997336884154461No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT110.18308921438082557No Hit
ACTTTACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAA110.18308921438082557No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAAACCTATA110.18308921438082557No Hit
TGTTTGAAATGTGCAATATTGGCAATCAAGACTTACCTCAGCATTTGCTG100.16644474034620504No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT100.16644474034620504No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA100.16644474034620504No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC100.16644474034620504No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT100.16644474034620504No Hit
CCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCGACTAA100.16644474034620504No Hit
GTGAATTAACTAACCCCTTTTAGCTTTAGTAGGCTCATCTGTGAAATGGA100.16644474034620504No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG90.14980026631158455No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC90.14980026631158455No Hit
ATCCTCTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTA90.14980026631158455No Hit
GTATTAGACTAATACCTTAGAAATTATGAAAAGTGGGACACTTGGGTGGC90.14980026631158455No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG90.14980026631158455No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG90.14980026631158455No Hit
CTTTTAGAGACAGATAATTAGAGCATTGGTGTCTAGCCCTTTTTTTCAAA90.14980026631158455No Hit
GGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAGTGAATTA80.13315579227696406No Hit
GTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTTTGGTTG80.13315579227696406No Hit
ACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAAGGGGT80.13315579227696406No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC80.13315579227696406No Hit
ATATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGA80.13315579227696406No Hit
ATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATC80.13315579227696406No Hit
GTCTTAGCCAAGTCACTTCACCACTTTTTGACTGTTTACTCATTTGCCAA80.13315579227696406No Hit
TGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAGGTAATCGTCAA80.13315579227696406No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT80.13315579227696406No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA80.13315579227696406No Hit
TTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTGCTCCT80.13315579227696406No Hit
CCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTATGAACCATGTTTAA80.13315579227696406No Hit
AGTTAATTCACTTGCTACACATTCCACAGCAGGAGTTCAGATATGCTCAT80.13315579227696406No Hit
CAATAAATGTGAACTGTTTTATTGGCAAATGAGTAAACAGTCAAAAAGTG70.11651131824234355No Hit
ATTCTATATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGC70.11651131824234355No Hit
CTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATA70.11651131824234355No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA70.11651131824234355No Hit
GTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGG70.11651131824234355No Hit
GCTGTGGAATGTGTAGCAAGTGAATTAACTAACCCCTTTTAGCTTTAGTA70.11651131824234355No Hit
TTATATAACAATGCATGGCAAATTTCAAAGGACTGAAATCACGCACAGAG70.11651131824234355No Hit
GGATAGGACAGGGTTGGTTATTGACTGAAACTGGGGATGAGGTAATGCTC70.11651131824234355No Hit
AACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTA70.11651131824234355No Hit
ATTTTTAACAGGATCAGCACTGGAGGAACGCAACAATGCATTTTATTAAG70.11651131824234355No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT70.11651131824234355No Hit
CATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGGATCAGC70.11651131824234355No Hit
GTGTAGCAAGTGAATTAACTAACCCCTTTTAGCTTTAGTAGGCTCATCTG70.11651131824234355No Hit
GTTCTGGAGACTGAATAGGAAAACCAGAAAAGAGAACATATCAGGCAGAG70.11651131824234355No Hit
CCTATGAACCATGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAG70.11651131824234355No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG70.11651131824234355No Hit
AATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAA70.11651131824234355No Hit
ACTTAGAAGTTTGTTGGTCAGTTTCTAAATACTTGGAGGGTTTTCTAAGT70.11651131824234355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)