FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5381
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA571.0592826612153874No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT480.8920275041813789No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC250.46459765842780154No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT150.27875859505668094No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG130.24159078238245676No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG120.22300687604534472No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC110.20442296970823265No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT110.20442296970823265No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA100.1858390633711206No Hit
GTGTAGCAAGTGAATTAACTAACCCCTTTTAGCTTTAGTAGGCTCATCTG100.1858390633711206No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC90.16725515703400856No Hit
TACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTT90.16725515703400856No Hit
ACTTTACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAA90.16725515703400856No Hit
GTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACACAAATCATTTG90.16725515703400856No Hit
TGTTTGAAATGTGCAATATTGGCAATCAAGACTTACCTCAGCATTTGCTG80.1486712506968965No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT80.1486712506968965No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA80.1486712506968965No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA80.1486712506968965No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC80.1486712506968965No Hit
GTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAGTACACTATAG80.1486712506968965No Hit
GTACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCAT70.13008734435978445No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA70.13008734435978445No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG70.13008734435978445No Hit
ACATACATCATCTCCATTTCACAGATGAGCCTACTAAAGCTAAAAGGGGT70.13008734435978445No Hit
GTGTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAA70.13008734435978445No Hit
GAGCAAAAGCAGGGGGAGCAGCAGGCAGATCAGGCCTAGGGGGAAGCAGG70.13008734435978445No Hit
GCGCTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACC70.13008734435978445No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT70.13008734435978445No Hit
GTATTAGACTAATACCTTAGAAATTATGAAAAGTGGGACACTTGGGTGGC70.13008734435978445No Hit
CCTTTAAACCTAATCCTCGCTGGGCAGTCGCCTTCACTTCTCGGCACTCC70.13008734435978445No Hit
GGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAG70.13008734435978445No Hit
CCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCGACTAA70.13008734435978445No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG70.13008734435978445No Hit
GTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAGTTTGGTTG60.11150343802267236No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA60.11150343802267236No Hit
AAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCG60.11150343802267236No Hit
CTTTTAGCTTTAGTAGGCTCATCTGTGAAATGGAGATGATGTATGTAAAG60.11150343802267236No Hit
GTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCACCCGCATAAT60.11150343802267236No Hit
CTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTA60.11150343802267236No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC60.11150343802267236No Hit
GTAGTATCCATTTCACATGGATACTTCATCAGAGTGTTTCCTCTTGAAAA60.11150343802267236No Hit
GTATTAGGGAACAGAACGAGTTACCAGGAAGCTCCCATACGGAATTTAGG60.11150343802267236No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG60.11150343802267236No Hit
GAGCAAAAGCAGGCAAGAAAATACCACAATAATTGGTGTCCTTTGTTAAG60.11150343802267236No Hit
CATCCAAATACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTA60.11150343802267236No Hit
CCTATGAACCATGTTTAAGCATTTCACCCTCCAGAAGCCCCAGTAGCTAG60.11150343802267236No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG60.11150343802267236No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA60.11150343802267236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)