FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4130
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT170.4116222760290557No Hit
CTATTATGTGTCCTAGAAACCTATGAACCATGTTTAAGCATTTCACCCTC110.26634382566585957No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC100.24213075060532688No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT80.1937046004842615No Hit
CAATAATTGGTGTCCTTTGTTAAGCGCTATTATGTGTCCTAGAAACCTAT80.1937046004842615No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT70.1694915254237288No Hit
GAGCATTACCTCATCCCCAGTTTCAGTCAATAACCAACCCTGTCCTATCC70.1694915254237288No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG70.1694915254237288No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC70.1694915254237288No Hit
CTCATACATATGGTAAATGTTCCATTCCCAGCCAAATGATTTGTGTAATG70.1694915254237288No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG70.1694915254237288No Hit
GGTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCA60.14527845036319612No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA60.14527845036319612No Hit
GTGTACTAGTGTAATTATGCGGGTGAAACCCTTTGTTAAAATTTTGGAAG60.14527845036319612No Hit
GGAATGTTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTT60.14527845036319612No Hit
GGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAAG60.14527845036319612No Hit
AGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAGAACT60.14527845036319612No Hit
GATAATTAGAGCATTGGTGTCTAGCCCTTTTTTTCAAATGTGTGTTTAAG50.12106537530266344No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG50.12106537530266344No Hit
AATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTTATTGACTGAA50.12106537530266344No Hit
TAATGGTACAAATCAGCACAGAGTTTTCCAAGGATAGGACAGGGTTGGTT50.12106537530266344No Hit
CTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAGAT50.12106537530266344No Hit
GTTATTATGTCTTAGGTGGAGGAAATGGTCTACGGATTCATGTTTGAAAT50.12106537530266344No Hit
GGGTTAAAGGTATGAGCATATCTGAACTCCTGCTGTGGAATGTGTAGCAA50.12106537530266344No Hit
ATTCCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTT50.12106537530266344No Hit
TCCAAATACATAACCACTGTACTTCCCTTCCTTCCTAGTCTAAAAGTATT50.12106537530266344No Hit
GAGCAAAAGCAGGCAAGAAAATACCACAATAATTGGTGTCCTTTGTTAAG50.12106537530266344No Hit
GTTTACTCATTTGCCAATAAAACAGTTCACATTTATTGAGTACACTATAG50.12106537530266344No Hit
GGGTTGGTTATTGACTGAAACTGGGGATGAGGTAATGCTCTTTGTTGACT50.12106537530266344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)