FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D2_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_D2_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCA60.5263157894736842No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC60.5263157894736842No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT40.3508771929824561No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA40.3508771929824561No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA40.3508771929824561No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG40.3508771929824561No Hit
TGGCCATATGGTCCACAGTGGTCTTAGTGCTGTCTCTTATACACATCTCC30.2631578947368421No Hit
CAATTAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCAC30.2631578947368421No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30.2631578947368421No Hit
GATGCCAGGGTCGACTTCGAGTCTGGACGGATCAAGAAAGAAGAGTTCTC30.2631578947368421No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCCTGTCTC30.2631578947368421No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.2631578947368421No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT30.2631578947368421No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACCTGTCTCTTATACA30.2631578947368421No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC30.2631578947368421No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGC30.2631578947368421No Hit
ATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGT30.2631578947368421No Hit
GATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGCT30.2631578947368421No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA30.2631578947368421No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.2631578947368421No Hit
TGCCAGGGTCGACTTCGAGTCTGGACGGATCAAGAAAGAAGAGTTCTCTG30.2631578947368421No Hit
CCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCCTG30.2631578947368421No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA30.2631578947368421No Hit
GGTCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTATG30.2631578947368421No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAG30.2631578947368421No Hit
GTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACCTGTCTC30.2631578947368421No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGGGCA30.2631578947368421No Hit
CAATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGAC30.2631578947368421No Hit
GTACACTTGTTTCCCTCTTACTGAGTTTGGAAATTCAAATTGTCCTGCTT20.17543859649122806No Hit
GTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGACTGTCTCTTATAC20.17543859649122806No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT20.17543859649122806No Hit
CCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTA20.17543859649122806No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT20.17543859649122806No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGCTGTCTCTT20.17543859649122806No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT20.17543859649122806No Hit
CAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGACACTGTCTCTTAT20.17543859649122806No Hit
CAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTAGTTGTCTG20.17543859649122806No Hit
AGGACAAACCCTCTGGAGCAAAACAAACGATAAGCATCAATGAACTGAGC20.17543859649122806No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC20.17543859649122806No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACCTGTCTCTTATAC20.17543859649122806No Hit
GTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTCTAAGTG20.17543859649122806No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACCTGTCTCTTATACACATCTC20.17543859649122806No Hit
CCAAAAGAATTCGGATGGCCATCAATTAGTTGTCCTGTCTCTTATACACA20.17543859649122806No Hit
GCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAGCTGTCTCTTA20.17543859649122806No Hit
ACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATT20.17543859649122806No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGCTGTCTCTTATACAC20.17543859649122806No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCCTGT20.17543859649122806No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG20.17543859649122806No Hit
CTGTAAGCTTTGAACTACTGACTTGGACATCATTCATGGAGACTTACCTA20.17543859649122806No Hit
GTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAA20.17543859649122806No Hit
GCCTGCAGTGTGTCAGGCGTTGTGCTAAATACTTTCCACGTAGGGAGGAT20.17543859649122806No Hit
GTGCAATATCCTCAAAGGAAAATTTCAAACAGCTGCCCAGAGGCTGTCTC20.17543859649122806No Hit
CCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAAGTATGTCC20.17543859649122806No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT20.17543859649122806No Hit
GGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGCTGTCTCTTA20.17543859649122806No Hit
CACTTGGGTGGCTCAGTCAGTTAAACATCTGCCTTCAGCTCAGGTCCTGT20.17543859649122806No Hit
TTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTCTGCTCTGGAGGTAGT20.17543859649122806No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGCTGCAATCTAT20.17543859649122806No Hit
GTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCC20.17543859649122806No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCCTGTCT20.17543859649122806No Hit
GTCGCACAAAGTCTTCCATTTTGGATCAGTACCTGCTCTGTCTCTTATAC20.17543859649122806No Hit
GTATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTC20.17543859649122806No Hit
CAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTG20.17543859649122806No Hit
GAAGAGAAGGTAATGGTGAAATTTCTCCAACTACTGTCTCTTATACACAT20.17543859649122806No Hit
AGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAACTGTCTCTTAT20.17543859649122806No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20.17543859649122806No Hit
GTGCCTGGCAGAGAGTAGGTGCTCAGTAAATAGTTGTTGAATAAATGAAT20.17543859649122806No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA20.17543859649122806No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC20.17543859649122806No Hit
CAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAA20.17543859649122806No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.17543859649122806No Hit
ATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAGCCATCTGTCT20.17543859649122806No Hit
ATGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCC20.17543859649122806No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG20.17543859649122806No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCTGTCTCTTAT20.17543859649122806No Hit
ATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTAC20.17543859649122806No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA20.17543859649122806No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACT20.17543859649122806No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20.17543859649122806No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA20.17543859649122806No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG20.17543859649122806No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG20.17543859649122806No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC20.17543859649122806No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.17543859649122806No Hit
GGGTACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTC20.17543859649122806No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCTGTCTCTT20.17543859649122806No Hit
AGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCT20.17543859649122806No Hit
GAGCAGCAGAGGGAGAGGGAGAAGCAGGCTTCCCACTGAGCAGGGTGATC20.17543859649122806No Hit
AGCAACCTCCATGGCCTCCGCTGCCTGTTCCTGTCTCTTATACACATCTC20.17543859649122806No Hit
AGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTC20.17543859649122806No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT20.17543859649122806No Hit
GTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTAT20.17543859649122806No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTCTGTCT20.17543859649122806No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA20.17543859649122806No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA20.17543859649122806No Hit
GAATTTGGGCAAAGAAGACAAGAGATATGGCCCAGCATTAAGCATCAATG20.17543859649122806No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTCTG20.17543859649122806No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGT20.17543859649122806No Hit
GTTCATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGC20.17543859649122806No Hit
GTTCAACTCCTTCCTCACACATGCACTGAAGTAGTTGTGGCAATGCTACT20.17543859649122806No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC20.17543859649122806No Hit
GTCATCTTTCAGACCAGCACTGGAGCTAGGATGAGTCCCAATAGTTCTCA20.17543859649122806No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA20.17543859649122806No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCTGTCTCTTATA20.17543859649122806No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20.17543859649122806No Hit
GCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCCT20.17543859649122806No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAACTC20.17543859649122806No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT20.17543859649122806No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20.17543859649122806No Hit
CCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCAT20.17543859649122806No Hit
AACATGCACTCTTTTTTATGATAGAGGCACTGAAACTAAAGAAAATGGTG20.17543859649122806No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACT20.17543859649122806No Hit
TACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTG20.17543859649122806No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCTGTCTCTT20.17543859649122806No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG20.17543859649122806No Hit
CTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCA20.17543859649122806No Hit
GTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATCAATAATTGTAG20.17543859649122806No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG20.17543859649122806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)