FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_C5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_C5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC140.4510309278350515No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC80.25773195876288657No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC70.22551546391752575No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATATT70.22551546391752575No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA60.19329896907216496No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT60.19329896907216496No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG60.19329896907216496No Hit
GTACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCAT50.1610824742268041No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTC50.1610824742268041No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG50.1610824742268041No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG50.1610824742268041No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG50.1610824742268041No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT50.1610824742268041No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC50.1610824742268041No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC50.1610824742268041No Hit
GCATGGAATTACTTTACATACATCATCTCCATTTCACAGATGAGCCTACT50.1610824742268041No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATATTCGAGAAATTTTTCCCT50.1610824742268041No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC50.1610824742268041No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC50.1610824742268041No Hit
ATGCTAGTGAATACTACTTTTGACATAAACCATACGATGATTTATAGGTT50.1610824742268041No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG50.1610824742268041No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA50.1610824742268041No Hit
TACAACTACTGCTTGAAGTAGAACAAGAGATAAGAGCTTTCTCGTTTCAG50.1610824742268041No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT50.1610824742268041No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT50.1610824742268041No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT50.1610824742268041No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT40.12886597938144329No Hit
CCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCG40.12886597938144329No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT40.12886597938144329No Hit
CTCTTAATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATA40.12886597938144329No Hit
ACACTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTT40.12886597938144329No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.12886597938144329No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA40.12886597938144329No Hit
CATTTATTGAGTACACTATAGATGCCAAGCATGGAATTACTTTACATACA40.12886597938144329No Hit
ATACTTAAGTCAACAAAGAGCATTACCTCATCCCCAGTTTCAGTCAATAA40.12886597938144329No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC40.12886597938144329No Hit
GCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACA40.12886597938144329No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC40.12886597938144329No Hit
GTCCTATCCTTGGAAAACTCTGTGCTGATTTGTACCATTATCAATTAATT40.12886597938144329No Hit
TCTCTGTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGAT40.12886597938144329No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT40.12886597938144329No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA40.12886597938144329No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA40.12886597938144329No Hit
ACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTG40.12886597938144329No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG40.12886597938144329No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC40.12886597938144329No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG40.12886597938144329No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.12886597938144329No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG40.12886597938144329No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC40.12886597938144329No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC40.12886597938144329No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA40.12886597938144329No Hit
GTATTTTGGGTAGTCATAAGTCCCATTTTTGACACTTTCCATGCACGTGT40.12886597938144329No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG40.12886597938144329No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)