FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_B6_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_B6_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA60.39164490861618795No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA50.3263707571801566No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT50.3263707571801566No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA50.3263707571801566No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC50.3263707571801566No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA50.3263707571801566No Hit
ATACAGTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAAGTGTA40.26109660574412535No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC40.26109660574412535No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA40.26109660574412535No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA40.26109660574412535No Hit
TCCCAGGACTTTGGAATCATGACCTGAGCTGAAGGCAGACACTTAACAAC30.19582245430809397No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.19582245430809397No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA30.19582245430809397No Hit
TTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTC30.19582245430809397No Hit
GGAGAGGAGGAAGCAGGCTCCCCACTCAGCAGGGAGCCCAATGTGGGACT30.19582245430809397No Hit
ACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATC30.19582245430809397No Hit
CATGCACACTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTG30.19582245430809397No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTCTGTCTC30.19582245430809397No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.19582245430809397No Hit
CCATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG30.19582245430809397No Hit
CCTTTTAGCTATTTGGAGGAATGCTGCTATGAACATGGGTATACAAGCTG30.19582245430809397No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACCTGTCTCTTATAC30.19582245430809397No Hit
GGGGATTAGGAGTACACTTTCTGTGAGAAGCTCTGAGTAACTGCTGAGTC30.19582245430809397No Hit
GAGCAAAAGCAGGGGATTAGGAGTACACTTTCTGTGAGAAGCTCTGAGTA30.19582245430809397No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT30.19582245430809397No Hit
AGCTAAAAGGGGTTAGTTAATTCACTTGCTACACATTCCACAGCAGGAGT20.13054830287206268No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA20.13054830287206268No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC20.13054830287206268No Hit
GTCTTCTGCCTTCTTCCTCAGTGCAGGCCCACTCATCTCCATCAGGTCAT20.13054830287206268No Hit
CTTTTAATTCCTCTGATGTGCGGTTTGCTCATGAGTGTCTCCAAGATGCA20.13054830287206268No Hit
GCTTCTGCCTTTGGATCACCTCATGATTCCAGGGTCAAGCCCCCTATCAG20.13054830287206268No Hit
CTATAGTCGGAACCACTGTGCAATCCTGCCAGCAAATGGGGGTCAAGGGG20.13054830287206268No Hit
GATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAA20.13054830287206268No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA20.13054830287206268No Hit
AAGATATGCATCTTGGAGACACTCATGAGCAAACCGCACATCAGAGGAAT20.13054830287206268No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT20.13054830287206268No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA20.13054830287206268No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA20.13054830287206268No Hit
AAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCT20.13054830287206268No Hit
CATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGA20.13054830287206268No Hit
CCATAGTGTGCATGTTCATTTTGTAGGTTCTCTAAGCTTTTGAGTTTAAT20.13054830287206268No Hit
TTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20.13054830287206268No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT20.13054830287206268No Hit
GCAACAGGCAGAGGGAGAGGGAGAAGCAAGATCCCCACTGACTGGGGAGC20.13054830287206268No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCCTGTCT20.13054830287206268No Hit
CTCTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGG20.13054830287206268No Hit
CCTCATTTCTTATCCTAGCATAGTTCTTTCATACCAATACCTTGCTGTCT20.13054830287206268No Hit
GTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAAGTGTACTCCC20.13054830287206268No Hit
GGCAGAAGACGGAGAACTGTCAGGAAACAAACTGACGTTAGATAATCTGT20.13054830287206268No Hit
GTTTCTGGATGTGCTCTAATGGGTCTCTACAGTGTAGAATCTGTCTCTTA20.13054830287206268No Hit
GTTCAGGCTGGGGAGTGACATGATCAGATCTGTGTTTTAGATACCTCTGG20.13054830287206268No Hit
CATGATCCCAGGGTCCTGGGATTGAGTCCTGTGTCTGGCTCCCCAGTCAG20.13054830287206268No Hit
TTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCT20.13054830287206268No Hit
GTTCTTTGTATGGATAGTTTCTGTCTTCAAGTGCCTTGAGCTTTTCTTTT20.13054830287206268No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT20.13054830287206268No Hit
ACACCTGGGTGACTCAGTTGTCAAGCCTCTGCCTCCGGATCACCTCATGA20.13054830287206268No Hit
GCTTAATGCTGGGCCATATCTCTTGCCTTCCTGATGTGTACTTTTTGATT20.13054830287206268No Hit
CCATTGTTCATAGTGGGATGTTCAAATCCCCTACTCTTATTGTATTGCTG20.13054830287206268No Hit
CCCTAATATCACTATTCTAAAGGCGTAAGGAACAGAAACTGTCTCTTATA20.13054830287206268No Hit
CGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAACTGTCTCTTATAC20.13054830287206268No Hit
GAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT20.13054830287206268No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC20.13054830287206268No Hit
ACCATGACCTGAGCCGAAGGCAGAGGCTTTAACCCACTGAGCCACCCAGG20.13054830287206268No Hit
GGGGAACTGTTGTGTCAGAGTATGAATAACACTAGCTCTGTCTCTTATAC20.13054830287206268No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT20.13054830287206268No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG20.13054830287206268No Hit
GCACTCTTTTTTATGATAGAGGCACTGAAACTAAAGAAAATGGTGGAAGA20.13054830287206268No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC20.13054830287206268No Hit
CCTGTTCTCAGCTCTAGACTAATCTCAGTCCAGAATACAAGCTGTCTCTT20.13054830287206268No Hit
GGACTGGTTTGCTAATCTTGTTTTTTTGCATCATCAACCTGTCTCTTATA20.13054830287206268No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT20.13054830287206268No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT20.13054830287206268No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC20.13054830287206268No Hit
ATTCCGTATGGGAGCTTCCTGGTAACTCGTTCTGTTCCCTAATACTCATG20.13054830287206268No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC20.13054830287206268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)