FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_B5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_B5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATTAAAAACAAAGATATGCATCTTGGAGACACTCATGAGCAACTGTC30.2926829268292683No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGCTGTCTCTT20.1951219512195122No Hit
CATGCGAACAATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGT20.1951219512195122No Hit
GTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAAGCTTACAGTGAGCGG20.1951219512195122No Hit
GATTTAGATGTACTGAGGGGTGCCTGGGTGGCTCAGTCATTAAGCTGTCT20.1951219512195122No Hit
CATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGCCAGAGAGTTCA20.1951219512195122No Hit
CTTTCGATCTTGGGGGGCTATATGAAGCAATCGAGGAGTGCCTGATTAAT20.1951219512195122No Hit
CCCAGGACCCTGGGATCACGCATGACCTGAGCCTAAGGCAGATGCTTAAC20.1951219512195122No Hit
ATTTTTGCACCACACCCAGTGGTCCATGCAATCCGTGCCCCTGTCTCTTA20.1951219512195122No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA20.1951219512195122No Hit
ATCCCAGACCCTGGGATCATAACCTGAGCCTTAAAGGCAGATGCTTAACT20.1951219512195122No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAAAACTACGTCCCCTTGC20.1951219512195122No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCGATCCCTGTCTCTTATA20.1951219512195122No Hit
CTATTCTTTTTTCCAGGTGGTTGAACTCTTTACCCTGTCTCTTATACACA20.1951219512195122No Hit
GTATAGTGACTCAGCAGTTACTCAGAGCTTCTCACAGAAAGCTGTCTCTT20.1951219512195122No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC20.1951219512195122No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC20.1951219512195122No Hit
CATTAGCGTTCTCTCCTTTTGCCTTCCTGATGCTGTCTCTTATACACATC20.1951219512195122No Hit
GATCGAGGCCTGCACCGGGTCCCTGTTCGTGAGAAGCCTGCTTCTCCCTC20.1951219512195122No Hit
GCCTCCCTGATGTGTACTTTTTGATTACTGTCTCTTATACACATCTCCGA20.1951219512195122No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC20.1951219512195122No Hit
TTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTC20.1951219512195122No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA20.1951219512195122No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT20.1951219512195122No Hit
GTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAG20.1951219512195122No Hit
CAACAGATGCACTACGCTCCTGTAAAATGGAGATTTCCTATACAGATGGC20.1951219512195122No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC20.1951219512195122No Hit
GTCCTGGGAGTGAGCCCCACATCCGGCTCCTGCTCAGCAGAGGAGCCTAC20.1951219512195122No Hit
GTCAATAACCAACCCTGTCCTATCCTTGGAAAACTCTGTGCTGATTTGTA20.1951219512195122No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA20.1951219512195122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)