FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_B1_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_B1_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773031
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC357330.9470635147180079No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG322300.8542203867394675No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT288590.7648757722902356No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG284340.7536116188814775No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA262300.6951970444981767No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC235200.6233715015858603No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT213820.5667061839672136No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA202440.5365447567221154No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA166900.44234993033452413No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA162040.4294690396129796No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG145310.38512803101803295No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA145060.38446543375869424No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA141840.37593118105841167No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA136500.36177810359893675No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG133560.3539859598291135No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA133560.3539859598291135No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA131180.34767803392020896No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC130720.34645885496302575No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC129040.3420062013802696No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC127320.3374475322360193No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA124780.33071554408113796No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA124620.33029148183516116No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG124030.3287277523031218No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT120750.3200344762605979No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA119850.31764912612697854No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT117900.31248086750413656No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC107210.2841482086948133No Hit
GAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGG106230.28155082743820553No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTG104910.2780523139088971No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT104880.27797280223777643No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA101640.2693855417567468No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG101550.2691470067433848No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA100620.26668214493864484No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT99090.2626270497114919No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA98500.26106332017945255No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT92190.24433936535374345No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC88760.23524853095561632No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC88530.23463894147702472No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG84550.22409039310835238No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT84140.2230037336030369No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACCTGTCTCTTA81650.21640426490002335No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC78300.2075254616248846No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT77570.2055906776276156No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA77230.2046895453549149No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT77150.20447751423192653No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA76870.20373540530146717No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA75680.2005814423470149No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG75260.19946827895132588No Hit
GGCAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGC75090.19901771281497557No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA75010.19880568169198717No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC74740.19809007665190134No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA74480.1974009755021891No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGCTGTCTCTTATAC74360.1970829288177065No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA73320.19432652421885746No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT73250.19414099698624263No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT71940.19066898734730778No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGCTGTCTCTTATAC71830.19037744455319874No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT71820.1903509406628252No Hit
TGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGAC71020.1882306294329413No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC70630.18719697770837293No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA70130.1858717831896955No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT70000.18552723261483936No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA69630.18454658867101806No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA68870.18253229300262838No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC68380.1812336023743245No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCTGTCTCTTA68050.18035897399199743No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC67330.17845069388510193No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA66670.17670143712044772No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCA66310.17574729706699999No Hit
TGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTC65170.17272585356441544No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT64830.1718247212917148No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA63430.16811417663941802No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC62660.16607337708065478No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT60510.16037504065034186No Hit
TGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGT59650.15809570607821669No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA58020.15377557194732827No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC56160.14884584833784825No Hit
GTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCC56160.14884584833784825No Hit
GCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGA55060.14593042039675794No Hit
CAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG54820.14529432702779277No Hit
GCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTA54810.1452678231374192No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA54700.1449762803433102No Hit
CCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCA54380.14412815585135663No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA53560.14195483684072566No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG53440.1416367901562431No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA53020.14052362676055405No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACCTGTCTCTT52450.13901290500926178No Hit
GGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATG52330.1386948583247792No Hit
ATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACA52310.1386418505440321No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTGTCTC52210.1383768116402966No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT52000.1378202299424521No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC51300.1359649576163037No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG50870.13482529033024113No Hit
GGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATA49660.13161831959504178No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCA49550.13132677680093272No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC49490.13116775345869142No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGCTGTC49490.13116775345869142No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG48910.1296305278170256No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTGCCATCATCCACTTCATTCTG48510.12857037220208367No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGACTGTC48430.1283583410790953No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC47880.12690062710855013No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA47760.12658258042406756No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG47660.12631754152033206No Hit
GCCATATCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGG47640.12626453373958496No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA47500.1258934792743553No Hit
ATGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGG47460.1257874637128611No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACCT46960.12446226919418367No Hit
CTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGC46140.1222889501835527No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA46130.12226244629317914No Hit
CATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGA46100.1221829346220585No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGCTGTCTCTTATA46030.12199740738944366No Hit
ACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATC45780.12133481013010496No Hit
ATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTA45570.12077822843226042No Hit
GGACTCTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGA45390.12030115840553657No Hit
GGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTAC45320.12011563117292172No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCCTGTCTCTT45150.1196650650365714No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCTGTCTC45140.11963856114619784No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG44530.1180218238334114No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA44470.1178628004911701No Hit
ACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTT44040.11672313320510751No Hit
CTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTG43500.1152919231249359No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCT43340.11486786087895912No Hit
ATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGA42830.11351616246990814No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC42060.11147536291114492No Hit
CTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCA41630.11033569562508233No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA41590.11022968006358813No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT41070.10885147776416361No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCTGTCTCTTATA40960.10855993497005459No Hit
CCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGAT40880.10834790384706618No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA39860.10564450702896425No Hit
AATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTA39630.10503491755037263No Hit
CATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTC39560.10484939031775778No Hit
CAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTG39520.1047433747562636No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTGTC39400.10442532807178101No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT39070.10355069968945392No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG38990.10333866856646554No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA38440.10188095459592036No Hit
CTCTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGG38300.10150990013069068No Hit
CTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGCTGTCTCTTA38260.1014038845691965No Hit
CTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCT38140.10108583788471391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)