FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_A9_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_A9_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238143
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA40491.7002389320702267No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC26301.104378461680587No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA23310.9788236479762159No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC22870.9603473543207233No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC22060.9263341773640207No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA21810.9158362832415816No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA21400.8986197368807817No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG16780.7046186534981083No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC16600.6970601697299522No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA12880.5408515051880592No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG12150.5101976543505373No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11510.48332304539709336No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG10760.4518293630297762No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA10690.4488899526754933No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA10020.42075559642735666No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG9490.39850006088778595No Hit
ATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAG9460.39724031359309325No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT9180.3854826721759615No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA8800.3695258731098542No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA8750.36742629428536633No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT8270.3472703375702834No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC7830.32879404391479067No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT7730.32459488626581506No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG7420.31157749755399067No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG7160.3006596876666541No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCT6880.28890204624952237No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC6820.286382551660137No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA6550.2750448260079028No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA6270.2632871845907711No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGA6080.2553087850577174No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCTCCTGTCT6020.25278929046833204No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA5850.24565072246507352No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTG5800.24355114364058567No Hit
CAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG5670.2380922386969174No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC5660.23767232293201984No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA5560.2334731652830442No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG5540.2326333337532491No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG5460.2292740076340686No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT5320.22339518692550275No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG5160.21667653468714176No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA5150.21625661892224418No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA5130.21541678739244907No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC5120.2149968716275515No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT5120.2149968716275515No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT5090.2137371243328588No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA5000.20995788244878077No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC4960.20827821938919053No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC4840.2032392302104198No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC4840.2032392302104198No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT4820.20239939868062465No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG4820.20239939868062465No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC4800.20155956715082954No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC4740.19904007256144415No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA4630.194420999147571No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA4570.19190150455818564No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT4430.18602268384961976No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA4390.1843430207900295No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA4380.18392310502513196No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT4200.17636462125697586No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT4070.17090571631330756No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA4010.16838622172392217No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC3990.16754639019412707No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCTCT3930.16502689560474168No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC3900.163767148310049No Hit
ACATTAGATCTCTCAGTTCTTTTATTCTCTCCATATTGAATATAATTGAC3880.16292731678025388No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCA3790.15914807489617583No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC3780.15872815913127827No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA3730.15662858030679047No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT3730.15662858030679047No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA3690.15494891724720022No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA3650.15326925418760998No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC3540.1486501807737368No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT3540.1486501807737368No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA3470.14571077041945385No Hit
ACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATC3450.14487093888965874No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC3440.14445102312476119No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA3420.14361119159496605No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT3320.13941203394599044No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA3280.1377323708864002No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA3270.1373124551215026No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT3200.13437304476721967No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG3170.133113297472527No Hit
GAATACTGGAGAATTGCACCTTTCGACCTTTTCGAAATAAGTTTTATATA3170.133113297472527No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCCTGTCTCT3160.13269338170762945No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCT3120.1310137186480392No Hit
ATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACA3080.12933405558844896No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG2950.12387515064478066No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT2950.12387515064478066No Hit
GCCATATCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGG2900.12177557182029283No Hit
ATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGA2900.12177557182029283No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA2880.12093574029049772No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT2870.12051582452560017No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT2860.1200959087607026No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT2820.11841624570111235No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA2820.11841624570111235No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC2810.1179963299362148No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTCTCTCCATA2760.115896751111727No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA2750.11547683534682941No Hit
GTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCCATATT2730.1146370038170343No Hit
ATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTC2690.11295734075744406No Hit
GGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCA2680.1125374249925465No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA2670.11211750922764893No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG2670.11211750922764893No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG2660.11169759346275138No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTG2650.11127767769785382No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG2630.11043784616805868No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC2630.11043784616805868No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA2620.11001793040316113No Hit
ATATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC2620.11001793040316113No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT2610.10959801463826357No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCTCCAGTATTCA2570.10791835157867333No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC2560.10749843581377574No Hit
CTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGC2530.10623868851908308No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA2520.1058187727541855No Hit
TGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGAC2520.1058187727541855No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA2480.10413910969459526No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGA2470.10371919392969771No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGG2460.10329927816480014No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG2440.10245944663500502No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGG2410.10119969934031232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)