FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_A6_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P3_A6_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10934184
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG1313351.2011413014450827No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1285221.1754146445678983No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA1043090.9539715080704697No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT987060.9027285438035431No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA879460.8043215662000933No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT828740.7579349314041176No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC752720.6884098529894869No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC713040.6521199935907426No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA684610.6261189678168942No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA546530.4998361103123928No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG528280.48314533576533925No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT518990.47464904559864735No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA515180.4711645606110159No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG505580.46238475591777134No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC495110.4528092814242014No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA482780.4415327197713153No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA474070.43356687613817363No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC461350.42193363491962455No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT450230.4117636944832829No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC430210.3934541434459124No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA411590.3764249805929734No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA407970.37311426257322905No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA401290.367004981807513No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA399490.3653587684275297No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC384140.3513202265482271No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA376510.3443421109430754No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT376150.3440128682670787No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG374130.3421654510295418No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA370130.33850719907402327No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC365120.33392523849973627No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC361040.3301938215051073No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA359160.32847444308601353No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT341870.31266164900828447No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT337830.30896681453321073No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT316970.2898890305851813No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG311290.2846943128083449No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA305900.2797648182982836No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG298580.2730702172196846No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT296060.2707655184877079No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC294570.26940281963427726No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA288930.26424468437699605No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC287930.2633301213881164No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC281330.2572940056615107No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA274790.25131276371423783No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA274180.2507548802910213No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC271640.24843189029926693No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA269620.24658447306173006No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA269080.24609060904773505No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT264510.2419110561885551No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA263860.2413165902457833No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG251600.23010404800211884No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA248320.22710428139859365No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG248270.22705855324914964No Hit
CAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG247950.22676589309270817No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA247800.2266287086443762No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC243780.22295216542908003No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA243170.22239428200586342No Hit
GAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGG236180.21600148671359473No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA233710.213742516131062No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA232210.2123706716477425No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA228230.20873071095200155No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT227470.20803564308045303No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA221880.2029232359726158No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT221720.20277690589439504No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC217850.1992375471274308No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT216820.19829554724888476No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA211750.19365871289526498No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA209460.1915643636507306No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC209460.1915643636507306No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA202130.1848606169422428No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC200660.1835162093485897No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT200540.18340646178992415No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA200360.18324184045192582No Hit
TGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTC199840.1827662676977084No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT196960.18013232628973502No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC196450.1796658991654064No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG194800.17815687023375498No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT191950.175550365715448No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA188870.1727335117096987No Hit
GGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATG187300.17129764781715764No Hit
CCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCA184770.16898380345529215No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC184570.1688008908575162No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGCCTTCCTGATGTGTA183860.16815155113541166No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT183630.16794120164796933No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA181920.16637729893698514No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT181460.16595659996210052No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG181320.16582856114365738No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA178860.16357873619101343No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGA172220.15750603794485257No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT169150.15469832956899207No Hit
ATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTA168480.1540855723664427No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCA165680.1515247959975797No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT162470.14858904880327603No Hit
GCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTG162250.14838784494572252No Hit
GATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAG161400.14761046640517483No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC160560.14684223349451592No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT159050.14546124338130764No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA158090.1445832629119832No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG157640.14417170956698733No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC156580.14320227279877493No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTGCCATCATCCACTTCATTCTG156270.14291875827222225No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC156070.1427358456744463No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA155810.14249805929733758No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA154310.14112621481401813No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG154000.14084270028746543No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG152910.1398458266295866No Hit
GGACTCTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGA152770.13971778781114347No Hit
GGCAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGC150660.1377880599046074No Hit
TGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGT146360.13385543905242495No Hit
GCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTA145780.13332499251887475No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCTGTCTC145110.1327122353163254No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGATATGGC144700.13233726449088473No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACCTGTCTC142650.13046241036368145No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCA140620.12860584749625578No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA139760.1278193233258193No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT139080.12719742049338112No Hit
GGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATT137520.1257707022307289No Hit
GTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCC135000.12346600349875216No Hit
GGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTAC134430.12294470259509077No Hit
GAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAACGACCTTG133840.12240511043165178No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA133150.12177406196932483No Hit
GTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCC131790.12053025630444851No Hit
AATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTA131100.11989920784212156No Hit
ACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATC130700.1195333826465697No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC130260.11913097493146264No Hit
CTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGG129800.118710275956578No Hit
GAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT128990.1179694799355855No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACCTGTCTCTTATAC128740.1177408391883656No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA128470.1174939071813681No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG127840.11691773249837391No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT127750.11683542182937473No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTCTGTCTC127740.11682627619948593No Hit
GCTTAATGCTGGGCCATATCTCTTGCCTTCCTGATGTGTACTTTTTGATT127340.1164604510039341No Hit
CTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGC126350.11555503364494323No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG126340.11554588801505443No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG126300.11550930549549925No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA125240.11453986872728683No Hit
CTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATC124770.11411002412251339No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA124050.11345153877052004No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCTGTCTCTTATAC122800.1123083350344205No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAACTGTCTC122400.11194250983886864No Hit
CTCTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGG122280.11183276228020307No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT122160.11172301472153752No Hit
GCCATATCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGG122110.11167728657209353No Hit
TACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAAT122030.11160412153298316No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT120630.11032373334855165No Hit
ATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT120550.1102505683094413No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC119820.10958293732755914No Hit
CATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGA118240.1081379278051293No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC117490.10745200556346957No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG117040.10704045221847373No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA116020.10610759796981649No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGCTGTCTCT115170.10533021942926879No Hit
ATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGA114300.1045345496289435No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGCTGTCTCT113810.10408641376439248No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA113570.10386691864706135No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC112980.10332732648362236No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGACTGTCTCT111490.1019646276301917No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA111020.10153478302541827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)