FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_G3_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_G3_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6079127
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCA438790.7217977186526947No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT293330.48251994077439075No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC262040.43104873446466907No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG233470.3840518548140218No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT221360.3641312313429214No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA192800.31715080142263846No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG178650.2938744329572322No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT174260.28665300132732874No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC168990.2779839934253718No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA161240.2652354523930821No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG158910.26140266521821304No Hit
GTACCAGTCTCTGTGTTTGTCGTCCACTTTCCCTTTTCTGAGTATTGGTG153590.2526514086644349No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT150880.24819353173572456No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA150810.24807838362317483No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA142620.23460605445485844No Hit
AATTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGA142240.23398096470101712No Hit
CCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAG140670.2313983570338307No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA130680.21496507639995022No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA130370.2144551347586586No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT127680.21003015729067678No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC125810.20695405771256303No Hit
GTATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTC122000.20068671044378575No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT121050.1991239860591825No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG115130.18938574568354963No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT114320.1880533175240458No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG110450.1816872718730831No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC109230.17968040476864525No Hit
CTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGG105290.17319921100513283No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA105200.17305116343185462No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC103400.17009021196629054No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC102760.1690374292229789No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA99560.16377351550642058No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT98960.1627865316845659No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC98450.1619475954359894No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC97320.16008877590482976No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC95380.15689752821416628No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG95250.1566836817194311No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG92780.15262059831946265No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT89960.14798177435674564No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT89730.147603430558368No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT88140.14498792343045308No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC86190.1417802260094254No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC85090.13997075566935846No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT84460.138934422656411No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA83700.13768424314872843No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA82720.13607216957303242No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA81760.13449299545806495No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA81390.1338843554345879No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA80460.13235453051071314No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC80300.13209133482488522No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT80080.13172944075687182No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA77910.12815984926783072No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT76790.1263174794670353No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC76420.12570883944355826No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG76390.12565949025246553No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT75830.12473830535206783No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG75350.12394871829458408No Hit
TAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACA74900.12320848042819306No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA74740.12294528474236514No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG74360.12232019498852385No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC74030.12177735388650378No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT72530.11930989433186706No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA72120.11863545538693303No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG71540.11768137102580684No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG71220.117154979654151No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT70920.11666148774322366No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA70240.11554290607845501No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA69360.11409532980640148No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA68710.11302609733272558No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG68220.11222006054487758No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT68050.11194041512868542No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT67320.11073958481209556No Hit
GGCTTGTACCAGTCTCTGTGTTTGTCGTCCACTTTCCCTTTTCTGAGTAT67230.11059153723881736No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT66770.10983484964206208No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT66380.10919331015785655No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC66140.10879851662911467No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA65920.1084366225611013No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG65580.10787733172871697No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG65390.10756478685179632No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG64580.1062323586922925No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC64190.10559081920808695No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT64020.1053111737918948No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT63370.10424194131821889No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC63360.10422549158785464No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG63010.10364975102510607No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC62520.10284371423725808No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTACCAG62210.1023337725959665No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG61920.1018567304154034No Hit
GTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTA61570.10128098985265482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)