FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_F8_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_F8_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441237
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT299410.6741590237134384No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC188630.42472401270186655No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT175350.39482243347968143No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC161830.3643804642715532No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA155800.35080316587473265No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG138190.31115205065615725No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA129130.29075232868680506No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC126160.2840650026107591No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT125670.2829617063894586No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT115070.25909448201030477No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT114570.25796866953959No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT113400.25533426835811734No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA113020.2544786508803741No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT108820.24502182612636975No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA107820.24277020118494017No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA106850.24058612499175341No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA105190.23684842758898028No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT104720.23579016386650833No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC101740.22908032154104813No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC100630.22658101785606125No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA99930.22500488039706054No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT98590.22198770297554488No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT95900.21593083188309925No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC89530.20158798100619263No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT88900.200169457293092No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG88800.19994429479894904No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA87070.19604898365027582No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA86270.19424768369713213No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC85780.19314438747583162No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG83420.18783055261405776No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT83100.18711003263280027No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT81570.183665046472413No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC80890.18213394151224083No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC80790.18190877901809788No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC80100.18035515780851144No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG79920.17994986531905413No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC78660.17711281789285283No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC75590.17020032932266393No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT73910.16641759942106218No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT72620.163513003246618No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG72410.16304016200891777No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT70540.15882962336844442No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA70290.15826671713308701No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC70220.15810910338718695No Hit
CTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTA69670.15687070966940067No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA69580.156668063424672No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT69100.15558728345278577No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG68290.1537634672502278No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT67360.15166945605469828No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG66660.15009331859569755No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG65310.1470536249247676No Hit
CATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATT65270.1469635599271104No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT64730.14574768245873843No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA64080.14428412624680917No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT63790.1436311550137946No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC63760.1435636062655517No Hit
GCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTT63710.1434510250184802No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA63290.14250534254307978No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG62990.14182985506065088No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG62010.1396232626180499No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC61740.1390153238838639No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA61500.1384749338979208No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC58830.13246309530430372No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA58330.13133728283358895No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA57060.12847771915797335No Hit
CTTCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTT56580.12739693918608713No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT55430.12480757050344307No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG54720.12320891679502805No Hit
TAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGC54520.12275859180674213No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT54350.12237581556669909No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA54070.12174536058309882No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC53920.12140761684188436No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT53550.12057451561355542No Hit
ATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTC53100.11956128438991209No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG52950.11922354064869765No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT52760.11879573190982604No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT52380.11794011443208277No Hit
AAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAG52210.11755733819203976No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT51780.116589139467225No Hit
CCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCG50750.11426996577755251No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50660.11406731953282384No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAG50600.11393222203633807No Hit
TTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGAC49710.11192827583846572No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG49440.11132033710427973No Hit
CTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGA49090.11053226837477938No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC48840.10996936213942196No Hit
GACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTTCAGTGT48380.10893361466636435No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT48350.10886606591812145No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG47870.10778528594623524No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA47480.10690715221907769No Hit
GAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGG47340.10659192472727756No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA47170.10620914848723452No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG47100.10605153474133446No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG47060.10596146974367728No Hit
AAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCT46690.1051283685153483No Hit
TAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTT46490.10467804352706239No Hit
TCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCT46050.10368732855283336No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT45170.10170589860437532No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA45130.10161583360671814No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA45050.10143570361140375No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC45030.10139067111257517No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG44710.10067015113131769No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT44670.10058008613366051No Hit
GAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGA44550.10030989114068896No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT44460.1001072448959603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)