FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_E5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_E5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC100.9861932938856016No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA100.9861932938856016No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT100.9861932938856016No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG80.7889546351084813No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC70.6903353057199211No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT60.591715976331361No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC50.4930966469428008No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC50.4930966469428008No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA40.39447731755424065No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC40.39447731755424065No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA40.39447731755424065No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.39447731755424065No Hit
GAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA40.39447731755424065No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA40.39447731755424065No Hit
GACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAGGAATGG40.39447731755424065No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA40.39447731755424065No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA30.2958579881656805No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA30.2958579881656805No Hit
GTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCT30.2958579881656805No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA30.2958579881656805No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG30.2958579881656805No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA30.2958579881656805No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG30.2958579881656805No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA30.2958579881656805No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.2958579881656805No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT30.2958579881656805No Hit
AGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTG30.2958579881656805No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA30.2958579881656805No Hit
CAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG30.2958579881656805No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT30.2958579881656805No Hit
AATGTTAACTTAGTTTTGGTAGAGGTAGCATTTCCCTTTTTTGCACTCAA30.2958579881656805No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT30.2958579881656805No Hit
CTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGG30.2958579881656805No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG30.2958579881656805No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG30.2958579881656805No Hit
GGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGG30.2958579881656805No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA20.19723865877712032No Hit
GATTGCTCAGTTCATTGATGCTTAATGCTGGGCCTGTCTCTTATACACAT20.19723865877712032No Hit
GCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGA20.19723865877712032No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA20.19723865877712032No Hit
GGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCGAGTGAT20.19723865877712032No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA20.19723865877712032No Hit
ATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGAT20.19723865877712032No Hit
TAATTGAATACTGGAGAATTGCACCTTTCGACCTTTTCGAAATAAGTTTT20.19723865877712032No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA20.19723865877712032No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATAAG20.19723865877712032No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTG20.19723865877712032No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC20.19723865877712032No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA20.19723865877712032No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCT20.19723865877712032No Hit
GTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGGAGTCTGCT20.19723865877712032No Hit
TCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTG20.19723865877712032No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC20.19723865877712032No Hit
GTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCAC20.19723865877712032No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA20.19723865877712032No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA20.19723865877712032No Hit
GCTTAATGCTGGGCCATATCTCTTGCCTTCCTGATGTGTACTTTTTGATT20.19723865877712032No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGCTGTCT20.19723865877712032No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC20.19723865877712032No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA20.19723865877712032No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT20.19723865877712032No Hit
TTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCCAGCATTAAGCATC20.19723865877712032No Hit
CCAGAGGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTC20.19723865877712032No Hit
GAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTG20.19723865877712032No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC20.19723865877712032No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG20.19723865877712032No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC20.19723865877712032No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT20.19723865877712032No Hit
CAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCAT20.19723865877712032No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT20.19723865877712032No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA20.19723865877712032No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA20.19723865877712032No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA20.19723865877712032No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC20.19723865877712032No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG20.19723865877712032No Hit
GGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCTCCAGTAT20.19723865877712032No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG20.19723865877712032No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC20.19723865877712032No Hit
CAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATTATCTGTCT20.19723865877712032No Hit
ATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGGAG20.19723865877712032No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT20.19723865877712032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)