FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_E4_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_E4_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTCAGAAATCCAAACAATAAAACTCATAATGTTCAAAAAAAAAAACA12.5No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCATGCAAA12.5No Hit
CCATACAAAAATCATTAAATATTAGAACAAATCAAAAATAATATACACAA12.5No Hit
GTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCT12.5No Hit
TATGAAACCACTAAATACAAAAAAACCGAAAATCTACAAAAACCCAAAAA12.5No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA12.5No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC12.5No Hit
GTTGGTAATGAAACGAAAACGGGAATCTAGCATACTTACTGACAGCCAGA12.5No Hit
AAAAAATCTACATAAAAAACAAATCCACAACTAATAAATCAAAATCTTAA12.5No Hit
ACATACTATGCTACATCAAAACGACTTATTCAATAACATCAGGACCAAAA12.5No Hit
ACCTTGTTCCTACTCCCTATAGTGAGTCGTCAAAAGCTGTCTCTTATACA12.5No Hit
GTGTACTCTTTGATTATGGCCATATGGTCCACAGTGGTCTTAGTGAGTAT12.5No Hit
GTCGTGGGATCCAGCCTCGCACCAGGCTCCCTGCTTGAGGATTGTTTTCC12.5No Hit
CGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTCTCTGGAATC12.5No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGA12.5No Hit
ATGATTCTGACTTTTTGTATTTAACAGAATATGATTTAGAGTTTCTGACT12.5No Hit
GCCATAATCAAAAAGAATACATCAGGAAGGCAAGAGATATGGCCCAGCAT12.5No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT12.5No Hit
CTATTGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGGAG12.5No Hit
CAACAGGTATAGATCATAAAAAAAATAAAACAAGAAAAAAACATAAATAA12.5No Hit
TTTTTAAACTATTCTACAACAAATTGATGGCCATACGAAATCTTTTGGTC12.5No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA12.5No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTACC12.5No Hit
TCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAACC12.5No Hit
GTACATCCCTTCCTTCCTAGTCTAAAAGTATTGCTCTTCGACTAACTAGA12.5No Hit
GGGTAGGGTCTATGCATCAGTGCAAAAAGAAAAGCCCAGGTGTGGGGGGT12.5No Hit
GGCCTGGAGTAACTGGAAGGCTGATACCTCTGGCCCTTTGCAGTGTTTGG12.5No Hit
GTGCAATTCTCCAGTATTCAATTACAAAAAGGCAACCAAACGACTTACAG12.5No Hit
GAAAATGCGTAAAAAAATCACAAAAACAAATTAATAAAGAATTCCTGATT12.5No Hit
CTGCTGAAGCTGCGTTAGTTCTGTTGACACTAATATGTTTGGTCCATTTG12.5No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTAACAACACTACGTCACCTTTC12.5No Hit
CAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATATTGCAA12.5No Hit
GCAAAGAGATAAGTCAAATCATTAATAATAAATGAACTCATCAATCTATA12.5No Hit
ATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAACT12.5No Hit
CAAGGGGACGTAGTGTTGGTAATGAAACGAAAAAGGGAATCAAGAAAAAT12.5No Hit
ATATAGGGAAGATCCGAAAATCGAAACTAACAAGTTTGTCTCCACAACTT12.5No Hit
CAATAAAAATGCCAAGAAATAATAAACAAGTAAATGATCAAACTTTCAAA12.5No Hit
ATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCT12.5No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC12.5No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)