FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_D4_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_D4_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG43.1746031746031744No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA32.380952380952381No Hit
GAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA21.5873015873015872No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA21.5873015873015872No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC21.5873015873015872No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA21.5873015873015872No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG21.5873015873015872No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC21.5873015873015872No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG21.5873015873015872No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA21.5873015873015872No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC21.5873015873015872No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC21.5873015873015872No Hit
GGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGC21.5873015873015872No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA21.5873015873015872No Hit
ATCCAGATGAAGGCACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTT21.5873015873015872No Hit
CAATATATTGAGAACACAATAGTCTGAATGTGCATGTCAAAATGGTTATT10.7936507936507936No Hit
GCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACACTGTCT10.7936507936507936No Hit
GGGCAAGGGGACGTATTGTTGGTAATGAAAAGAAAACGGTACTCTAGCAT10.7936507936507936No Hit
TCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTT10.7936507936507936No Hit
GTCCTAATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGG10.7936507936507936No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGC10.7936507936507936No Hit
AAAGCAGGGTGAAGACACTAGCCTGTTAGAGATGGTCACCTTCTCAACCT10.7936507936507936No Hit
CTGGAGAATTGCACCTTTCGACCTTTTCGAAATAAGTTTTATATACCTTA10.7936507936507936No Hit
AAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAACGACC10.7936507936507936No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA10.7936507936507936No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT10.7936507936507936No Hit
GGCAAGGGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA10.7936507936507936No Hit
GTATTCTTGAAGAACCTTTCAGTCAACTTGGTCCAGCTGTTCTCATAGAT10.7936507936507936No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA10.7936507936507936No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC10.7936507936507936No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC10.7936507936507936No Hit
GTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAA10.7936507936507936No Hit
AGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAA10.7936507936507936No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT10.7936507936507936No Hit
ACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGG10.7936507936507936No Hit
GGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGAC10.7936507936507936No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCTGTCTCTTAT10.7936507936507936No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT10.7936507936507936No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT10.7936507936507936No Hit
GATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACG10.7936507936507936No Hit
CAGTTCATTGATGCTTAATGCTGGGCCATATTATTCTCTTGTCTGCTGTA10.7936507936507936No Hit
CCACTGAGCCACCCAGGCCCTTCTTCTTACCCTCTGAGGCATTATGATCC10.7936507936507936No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC10.7936507936507936No Hit
CTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCATGTC10.7936507936507936No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA10.7936507936507936No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC10.7936507936507936No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC10.7936507936507936No Hit
ATATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC10.7936507936507936No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTGTCTCTTA10.7936507936507936No Hit
GAGTATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTCTGTCT10.7936507936507936No Hit
CTCTTCCACTGTTAAACAAATTCTGGTCCGTCCCTCATTCATCCTGTCTC10.7936507936507936No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC10.7936507936507936No Hit
TTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGAT10.7936507936507936No Hit
TCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTC10.7936507936507936No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG10.7936507936507936No Hit
AGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGA10.7936507936507936No Hit
GAGAACAATGGACATGATTCCAGAGAGGAATGAACAAGGACTGTCTCTTA10.7936507936507936No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG10.7936507936507936No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCTGTCTCTTAT10.7936507936507936No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT10.7936507936507936No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG10.7936507936507936No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA10.7936507936507936No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT10.7936507936507936No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTGCCATCATCCACT10.7936507936507936No Hit
AGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAACTGTCTC10.7936507936507936No Hit
ATACTGGAGAATTGCACCTTTCGACCTTTTCGAAATAAGTTTTATATACC10.7936507936507936No Hit
ACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATCTGTCTCTTA10.7936507936507936No Hit
AGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAG10.7936507936507936No Hit
CTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGA10.7936507936507936No Hit
GGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGG10.7936507936507936No Hit
TGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGT10.7936507936507936No Hit
GTCCTGAGATCCACCAATGTAAAAAGACATCCCTTTACCTGTCTCTTATA10.7936507936507936No Hit
ACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCTGT10.7936507936507936No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG10.7936507936507936No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA10.7936507936507936No Hit
GGCAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGC10.7936507936507936No Hit
GCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGG10.7936507936507936No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCCTGTCTCTTATACACAT10.7936507936507936No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT10.7936507936507936No Hit
ATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTT10.7936507936507936No Hit
GAATAGTGGTTGGAAGGGGCTGGGGAAAGAGGAAAATGGGGAATTGTTCA10.7936507936507936No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC10.7936507936507936No Hit
ACAATAACCCAGGCATGAGAAGATGGTATCTCACTCCTTACTGTGCGTTT10.7936507936507936No Hit
GAATTGCACCTTTCGACCTTTTCGAAATAAGTTTTATATACCTTAGGGTA10.7936507936507936No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAAGAAGACAAGAGAT10.7936507936507936No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCCTGTCTCTTATAC10.7936507936507936No Hit
TTATAAAACAATATGGAACAGCTGGGGTAGTAAACTTTAGTAGCAGTATA10.7936507936507936No Hit
GTCTTATTTCAGGAATAAAACACTTGAGCCTCTTTATTGTTGCACAGAGC10.7936507936507936No Hit
CAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCAT10.7936507936507936No Hit
ATGGCCATATGGTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGA10.7936507936507936No Hit
CTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTC10.7936507936507936No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC10.7936507936507936No Hit
GATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT10.7936507936507936No Hit
TGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACC10.7936507936507936No Hit
ATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGAATCTCATTGCCAT10.7936507936507936No Hit
CCATCAATTAGTTGTCGAATAGTTTAAAAACGACCTTGTTTCTACCTGTC10.7936507936507936No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG10.7936507936507936No Hit
AGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCAT10.7936507936507936No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA10.7936507936507936No Hit
TTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCCAAAGGG10.7936507936507936No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA10.7936507936507936No Hit
GAATAGGACTGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA10.7936507936507936No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG10.7936507936507936No Hit
GGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATATGGCCCAGCA10.7936507936507936No Hit
GGTTAAGTGTCTGCCTTTGACTCAGTTCATGATCGCAGGGTCCCGGGATC10.7936507936507936No Hit
GTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTAT10.7936507936507936No Hit
ATTGAGTATCTCATTGCCATCATCCACTTCCTGTCTCTTATACACATCTC10.7936507936507936No Hit
AGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATTATTCCTGTCT10.7936507936507936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)