FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_B6_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_B6_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6054295
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA546780.9031274491910289No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTTCTGTTTACTGTGT445050.7350979758997538No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA286420.4730856358998034No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC276240.4562711265308347No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG250970.4145321627043281No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG232780.38448737631714347No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA223950.3699026889175371No Hit
GGATACACCATGGACACAGTAAACAGAACATTCGAGAAATTTTTCCCTAG210540.3477531240218721No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG208100.343722927277247No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT196600.32472814753823526No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTTCTGTTTACTGT192330.3176753032351413No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT191630.31651909925102756No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA189990.3138102784882468No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG188870.31196035211366474No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA177340.2929160207753339No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC173020.2857805904733747No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT165120.2727320026526623No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA164600.2718731082644635No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG163390.269874527091924No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG153930.2542492561066152No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA143210.2365428179499017No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC136310.22514595010649463No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCAGTCTTCTGTC135760.2242375041189767No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA132190.21834086379999654No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA128140.21165139789190981No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG127080.2099005747159661No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT126640.20917381792595174No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC122110.20169152642875843No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT119330.19709974489184953No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT119150.19680243529593455No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA118620.19592702370796272No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC117020.1932842717442741No Hit
AATGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCT111860.18476139666137842No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA110590.1826637122902006No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG107500.177559897560327No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC106200.17541266158983002No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATAAGATCTGTT105330.17397566520957436No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT105190.1737444244127516No Hit
ACCATGGACACAGTAAACAGAACATTCGAGAAATTTTTCCCTAGCAGTTC104700.17293508162387197No Hit
CTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGG102750.16971422766812652No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA101690.1679634044921828No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATAAGATCTGTTCCACCATTGA101580.16778171529467922No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC101070.1669393381062535No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA100380.1657996513219128No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT98840.1632560025568625No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC96570.15950659820837934No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG95240.1573098106385632No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT94720.15645091625036442No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG90850.15005875993819262No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA90280.14911727955112858No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA90090.14880345275544055No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACATTCGAGAAA88780.14663969958517054No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA87610.14470718721172324No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA87580.14465763561240408No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA83960.13867840929455866No Hit
GCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAGTAGT83230.13747265371112574No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC80760.13339290536718149No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC80450.13288087217421682No Hit
CATAAGATCTGTTCCACCATTGAAGAACTCAGACGGCAAAAATAATGAAT80280.1326000797780749No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC79870.1319228745873797No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA79670.13159253059191864No Hit
GTCCCTAAGTGCCTGAACAATGTTTTCAAAGAATTCCTTGGTCATGTCTT78860.13025463741030127No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT77760.12843774543526537No Hit
GTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTA76300.12602623426839954No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACATTCGAGAAATTTTTC76070.1256463386736193No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT75580.12483699588473968No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT74480.12302010390970376No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT74080.12235941591878163No Hit
ATATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC73630.12161614192899421No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT73110.12075724754079542No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG72530.1197992499539583No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC72270.11936980275985892No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCT70910.1171234635907236No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG70890.11709042919117751No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG70820.11697480879276612No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC70740.1168426711945817No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG70700.1167766023954895No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG70580.11657839599821283No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT70430.11633063800161703No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT69760.11522398561682244No Hit
GTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCG69730.11517443401750328No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG68630.11335754204246737No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA67100.11083041047719015No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC66830.11038444608331771No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT66800.11033489448399854No Hit
GTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAG66690.11015320528649496No Hit
CTTCAATGGTGGAACAGATCTTATGATCTCAGAGAACTCTTCTTTCTTGA66630.11005410208785665No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG66020.10904655290170037No Hit
GTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTTCTGTTTACT65730.1085675541082818No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA64850.10711404052825307No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC64110.1058917677450471No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC63910.10556142374958603No Hit
CTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTT63840.10544580335117466No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG63350.10463646056229503No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA63220.10442173696524533No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCTGT62310.10291867178589746No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC61880.10220843219565613No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC61720.10194415699928729No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATGTTCTGTTTACTGTGTCC61400.10141560660654958No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT60800.10042457462016634No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC60780.10039154022062023No Hit
ATATGAACTGCTAGGGAAAAATTTCTCGAATGTTCTGTTTACTGTGTCCA60760.10035850582107414No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT60550.10001164462583999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)