FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_B5_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_B5_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT70.6796116504854369No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT50.48543689320388345No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA50.48543689320388345No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA50.48543689320388345No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA50.48543689320388345No Hit
GGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTC40.3883495145631068No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC40.3883495145631068No Hit
GTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTG40.3883495145631068No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT30.2912621359223301No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30.2912621359223301No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT30.2912621359223301No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA30.2912621359223301No Hit
TCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATG30.2912621359223301No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA30.2912621359223301No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA30.2912621359223301No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA30.2912621359223301No Hit
GCTTAATGCTGGGCCATATTATTCTCTTGTCTGCTGTAATTGGGTATCTC30.2912621359223301No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.2912621359223301No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.2912621359223301No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC30.2912621359223301No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA30.2912621359223301No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC30.2912621359223301No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC30.2912621359223301No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG30.2912621359223301No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT30.2912621359223301No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG20.1941747572815534No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT20.1941747572815534No Hit
GTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTA20.1941747572815534No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT20.1941747572815534No Hit
GTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGAT20.1941747572815534No Hit
GTCTCCAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT20.1941747572815534No Hit
ATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCATAA20.1941747572815534No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.1941747572815534No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA20.1941747572815534No Hit
ATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGTCTGTCTCTTA20.1941747572815534No Hit
CTCCAACACCATGTCAAGCTTTCAGGTGGACTGTTTCCTTTGGCATATCC20.1941747572815534No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG20.1941747572815534No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT20.1941747572815534No Hit
GCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGG20.1941747572815534No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG20.1941747572815534No Hit
GGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATG20.1941747572815534No Hit
GTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCA20.1941747572815534No Hit
AAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTG20.1941747572815534No Hit
CATTGGTACTGGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCT20.1941747572815534No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG20.1941747572815534No Hit
GTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTT20.1941747572815534No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT20.1941747572815534No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCCTGTCTCTTATACAC20.1941747572815534No Hit
GGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGA20.1941747572815534No Hit
CTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACA20.1941747572815534No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC20.1941747572815534No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA20.1941747572815534No Hit
GAATGGGGCATCACCCAATCCATTGTCTGCAAATCGCTTGCGGATATGCC20.1941747572815534No Hit
GGGAAGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATCTGTCTC20.1941747572815534No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.1941747572815534No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC20.1941747572815534No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA20.1941747572815534No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC20.1941747572815534No Hit
TCAGTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTC20.1941747572815534No Hit
TACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAAT20.1941747572815534No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGTTCCTG20.1941747572815534No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA20.1941747572815534No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG20.1941747572815534No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCTGTCTCTTATAC20.1941747572815534No Hit
GTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCC20.1941747572815534No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC20.1941747572815534No Hit
ACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGA20.1941747572815534No Hit
GAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGG20.1941747572815534No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT20.1941747572815534No Hit
ATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCA20.1941747572815534No Hit
GGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTC20.1941747572815534No Hit
GAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT20.1941747572815534No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT20.1941747572815534No Hit
CAAGAGTGGCTGTTTCGATATCGAGGCCAAGGGTGTTGCCTCTTCCTTTT20.1941747572815534No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA20.1941747572815534No Hit
GCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGT20.1941747572815534No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA20.1941747572815534No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC20.1941747572815534No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG20.1941747572815534No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG20.1941747572815534No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA20.1941747572815534No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT20.1941747572815534No Hit
CAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTCTAAGTGTC20.1941747572815534No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC20.1941747572815534No Hit
AGGTGGCACAGGAGGTCCTTTAAACCTAATCCTCGCTGGGCAGTCGCCTT20.1941747572815534No Hit
ATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGAC20.1941747572815534No Hit
GGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTT20.1941747572815534No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT20.1941747572815534No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20.1941747572815534No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG20.1941747572815534No Hit
AAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGT20.1941747572815534No Hit
TATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTC20.1941747572815534No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT20.1941747572815534No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT20.1941747572815534No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC20.1941747572815534No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC20.1941747572815534No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT20.1941747572815534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)