FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_B1_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_B1_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3802864
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA323670.8511216809225888No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC166710.43838012613651184No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT122900.323177478868558No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT119590.31447351259471806No Hit
GTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCC116340.30592732214457313No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG115270.3031136532886793No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC104570.27497696472974054No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG103910.27324143066909573No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT103340.27174256034399336No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA101420.2666937339857539No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG83300.21904543522986886No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG79980.21031517298541308No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA78840.20731743233520838No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT78810.2072385444233609No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC75520.19858717009075266No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT74540.19601016497040125No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA73350.1928809444671174No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG71900.18906802872782197No Hit
AATCTGTGTTTGTCGTCCACTTTCCCTTTTCTGAGTATTGGTGTGTTCTG71570.18820026169749957No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG70070.18425586610512498No Hit
GGATACACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG64190.1687938353830166No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC63420.16676904564559764No Hit
GTGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAA62690.16484943979064198No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA61010.16043171672718246No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG60130.15811767131298937No Hit
CTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGG59980.1577232317537519No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA57300.1506759116287093No Hit
TTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC56580.1487826017443695No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG56470.14849334606759537No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA55830.1468104039481822No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA55670.1463896684183289No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT55450.14581115706478065No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG55260.14531153362307986No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT54460.1432078559738134No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT54400.14305008015011844No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA53830.1415512098250161No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA52420.13784347796818397No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT51290.13487203328859512No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT50990.1340831541701202No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG49850.1310854135199155No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG49620.13048060619575141No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG49090.1290869197531124No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC48940.12869248019387494No Hit
AGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGT48650.12792989704601584No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC48420.12732508972185175No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT47840.12579992342613358No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG47810.12572103551428607No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC47460.12480067654273201No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC46920.12338069412947715No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT46220.121539976186369No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA45700.12017258571434583No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT45620.11996221794941916No Hit
GTATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTC45320.11917333883094425No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC45250.11898926703663344No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG44720.11759558059399441No Hit
GAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAA44550.1171485490935253No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC44470.11693818132859866No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC44250.11635966997505039No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA43350.11399303261962564No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG43200.11359859306038816No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC43130.11341452126607736No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA42740.11238897841205997No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA42680.11223120258836497No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG42430.11157380332296922No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT41680.10960160552678191No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC41370.10878643043769118No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT40280.10592016964056564No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG40180.10565720993440733No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT40170.1056309139637915No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA40120.10549943411071236No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA40100.10544684216948069No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG39780.10460537110977411No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG39340.10344834840267755No Hit
GTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTA39190.10305390884344011No Hit
GTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT39160.10297502093159262No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC38920.10234391763681268No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA38410.10100282313540532No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG38330.10079245537047868No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC38300.10071356745863119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)