FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_A4_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_A4_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3261167
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA353301.08335451695666No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAA200320.6142586380887578No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT197360.6051821326537402No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG173490.5319874756490545No Hit
CAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAGAGTTCTCTGAG156150.4788163255668906No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA154380.4733888206277078No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG125910.3860887835550893No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCATTGGTGTG124670.3822864637106901No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT124600.3820718166226998No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG122770.3764603284652396No Hit
CTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGG120920.37078751256835357No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC120130.3683650668610347No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG111140.3407982479891401No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT108740.33343891925804475No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCATTGGTGTGTTCTGTTTAC103300.3167577741342286No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCATTG100830.30918379831514303No Hit
GTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAG97900.30019928448926414No Hit
TCATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTC95920.29412783828611044No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT87730.2690141289912476No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATGA87420.2680635490301478No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA85090.26091886738704273No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT78330.2401900914611242No Hit
ACCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAGA77060.23629578000758625No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG76060.2332293930362965No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC75540.23163487181122586No Hit
CTTCTTTCTTGATCCGTCATTGGTGTGTTCTGTTTACTGTGTCCATGGTG74310.2278632158365395No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC73390.22504213982295296No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCA73020.22390757664357575No Hit
AAACAGAACACACCAATGACGGATCAAGAAAGAAGAGTTCTCTGAGATCA70150.21510704603597422No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA68120.20888228048425608No Hit
GTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTA66970.2053559354672729No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA66850.20498796903071814No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA66320.2033627839359346No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA65930.20216689301713162No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG65900.20207490140799292No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC62740.19238511857871737No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC62550.19180250505417232No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT62530.19174117731474652No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATGACGGATC62180.19066794187479513No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT56380.17288289744131471No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG55700.1707977543008377No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC54050.16573821579820966No Hit
GTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCG53690.16463431648854535No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA51810.15886950898252067No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT51760.1587161896339562No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT51310.1573363154968758No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC51020.1564470632752018No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA50870.15598710522950834No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT50260.1541166091770216No Hit
CACCAATGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTG49570.15200080216683168No Hit
TTCTTGATCCGTCATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCC48760.14951702872008701No Hit
CACCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAG48630.14911839841381935No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA48060.1473705578401842No Hit
ATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCG47840.14669595270650046No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA46290.1419430529010014No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT45160.138478035623444No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG44680.13700616987722494No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT43650.13384779129679653No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG43640.13381712742708363No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA43440.13320385003282567No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA43320.1328358835962709No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT42830.13133335398033893No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT42080.12903356375187164No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT41880.12842028635761368No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT41420.12700974835082043No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC41370.12685642900225594No Hit
CCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAAAGAAGAG41180.12627381547771088No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC40870.1253232355166111No Hit
CTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTT40790.1250779245589079No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT40650.12464863038292734No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC40410.12391269750981782No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA39530.12121427697508284No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC39360.12069299118996359No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC39310.12053967184139912No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG39200.12020236927455724No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT39190.12017170540484434No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA39040.11971174735915088No Hit
GGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGG37850.11606274686331612No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA37510.1150201752930776No Hit
GCTGTATGGAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTT37470.11489751981422602No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT37160.11394693985312619No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC36800.11284304054346189No Hit
GTCATTGGTGTGTTCTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTT36660.11241374636748133No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG36270.11121785544867835No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA36220.11106453610011387No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT36180.11094188062126227No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT35750.10962333422360768No Hit
GTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAGG35700.1094700148750432No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT35680.10940868713561741No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC35180.10787549364997254No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC35180.10787549364997254No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG34800.10671026660088244No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC34720.10646495564317927No Hit
GAACACACCAATGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAG34670.10631163629461478No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT34650.10625030855518898No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG34500.10579035050949552No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC34480.10572902277006972No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC34200.10487043441810862No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG33580.102969274495909No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA32970.10109877844342226No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCATTGGTGTGTTC32680.10020952622174822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)