FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_A3_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P2_A3_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5448944
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC602211.105186619645935No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG453220.8317574928279682No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA408440.7495764316902505No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT402800.739225802283892No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT347300.637371204402174No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA341820.6273142098725918No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA311340.5713767658467402No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC230070.42222860062426776No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC223430.4100427532380586No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC212300.3896167771223195No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA211530.3882036592778344No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG206900.37970660003112533No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT200760.3684383616348415No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG197040.36161135074979667No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT177590.32591636104169913No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA175520.32211745982340795No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA174890.320961272496102No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC174230.3197500286294005No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT170320.312574326328184No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT166600.30574731544313904No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA164570.302021822944042No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC160180.2939652160124971No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA157940.28985432773763137No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA155940.2861838917779298No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA155120.2846790130344522No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA155080.2846056043152581No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA154850.28418350417989247No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC153630.2819445382444745No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT152120.2791733590948999No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG152120.2791733590948999No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT151290.2776501281716237No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC150230.2757047971129819No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA150110.2754845709553998No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA148450.27243810910884747No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA145290.26663882029251906No Hit
ACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTT145080.26625342451675044No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG144250.2647301935934742No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG142710.26190395790450405No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA141380.25946311799130256No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC135090.24791959689804116No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC134210.24630460507577248No Hit
GCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAC132630.24340496066760825No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT131780.2418450253847351No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC131470.24127610781098136No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC130780.2400098074048843No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGAGGTGA127580.23413710986936184No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT123320.22631908127519756No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA121570.2231074498104587No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC119260.2188680962770034No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA117950.21646396072339888No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG117590.2158032822506526No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT116590.21396806427080184No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA116470.21374783811321973No Hit
GCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTT113860.20895791918580922No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC112990.20736127954333905No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA110600.2029751085714957No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATG109850.2015986950866076No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC108860.19978182928655536No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG108430.1989926855552195No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA106140.19479003638136122No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC103430.18981659565596565No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC102610.188311716912488No Hit
GGGTTTGTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTG101940.18708212086598797No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA101740.18671507727001782No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA99410.18243901937696552No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA98480.1807322666557043No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA97820.17952102278900278No Hit
CTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAA97120.17823637020310726No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC96960.17794273532633112No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC96300.1767314914596296No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC95440.17515320399695794No Hit
GAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGG94870.174107129748443No Hit
ACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGA94740.17386855141106242No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA94710.17381349487166686No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA94670.17374008615247286No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC93090.17084044174430862No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA92680.1700880023725698No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG91060.16711494924521156No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC88340.16212315634001745No Hit
CAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG88260.16197633890162938No Hit
GAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGA88200.16186622582283836No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT86500.15874635525709202No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG85570.1570396025358308No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT84570.15520438455598No Hit
GATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGA84550.155167680196383No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA84460.15500251057819645No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA83790.15377291453169642No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA83760.1537178579923009No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG83400.1530571795195546No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG81610.14977213933562172No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA81610.14977213933562172No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGA81520.14960696971743515No Hit
TGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTC78270.14364251128292013No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT77890.14294512845057686No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT77210.14169718022427832No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC75810.13912787505248725No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA75610.13876083145651708No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTT75200.13800839208477828No Hit
CTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGG75070.13776981374739766No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG74130.13604470884633793No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA73570.1350169867776215No Hit
ATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTT73320.1345581822826588No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT73200.1343379561250767No Hit
CTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT72490.13303495135938265No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA72160.13242932942603192No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG71970.13208063800986025No Hit
GAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT71870.13189711621187516No Hit
CCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCA71750.1316768900542931No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA71570.13134655081791993No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG71470.13116302901993487No Hit
GTCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCT70880.13008025041182292No Hit
CTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATT70790.12991508079363634No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA70620.12960309373706172No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC70070.1285937238481438No Hit
CTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCC69610.12774952357741243No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCA68770.12620794047433778No Hit
ATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTA68740.12615288393494226No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA68420.12556561418139No Hit
GAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCA67170.12327159170657655No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA66930.12283113939141235No Hit
ATGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGG65480.12017007332062873No Hit
TCCTTGTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTG65410.12004160806203919No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA65340.11991314280344963No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGA65150.11956445138727796No Hit
GTGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAG64400.11818803790238991No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG64080.11760076814883766No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT63590.11670151133871076No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT62900.11543521093261373No Hit
CAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAA62890.11541685875281524No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT62870.11538015439321822No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC62280.11429737578510625No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA61620.11308613191840473No Hit
TTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTC61520.11290261012041967No Hit
TGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGT60880.11172807061331518No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT60110.11031495276883008No Hit
GGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATG58850.1080025781142181No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG57360.10526810332424044No Hit
GGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGC56700.10405685945753892No Hit
CTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGA56510.10370816804136729No Hit
GGCAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGC56440.10357970278277771No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT55950.10268044597265084No Hit
TGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAAC55650.10212988057869561No Hit
GTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCC54730.10044148003723291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)