FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_E6_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_E6_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA24.545454545454546No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC12.272727272727273No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCCTGTCTCTTATACA12.272727272727273No Hit
GAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGGGTAAAGCTGGAATC12.272727272727273No Hit
GGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTG12.272727272727273No Hit
GCCACTGCCTTCAGCTCAGGTCATGATCTCAGGGTCCTGGGATCGAGTCC12.272727272727273No Hit
GAGCAAAAGCAGGGGGAGCTGTGGAGGAAGAGGGAGAAGCAGACTCCCCA12.272727272727273No Hit
GATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTCTGTCTCTTATA12.272727272727273No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA12.272727272727273No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA12.272727272727273No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGAA12.272727272727273No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAAATGATGGCCATCCGAA12.272727272727273No Hit
CCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGCTGTC12.272727272727273No Hit
GAGCTCATAACTCTGTTTTTAAGGAGAAGTGGAAAAATAACTTGAAAGCC12.272727272727273No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT12.272727272727273No Hit
GAGGATCTCCAGTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGA12.272727272727273No Hit
CATTTTGGGCAAAGAAGACAAGAGATATGGCCCAGCATTAAGCATCAATG12.272727272727273No Hit
GTGTATACCTTAGGTTAATCCTATCGTATTAGACTAATACCTGTCTCTTA12.272727272727273No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACCTGTCTC12.272727272727273No Hit
ATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAACT12.272727272727273No Hit
GTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGG12.272727272727273No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGCTGTCTCTTATAC12.272727272727273No Hit
TAAAAAAGAGTGCATGTTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGT12.272727272727273No Hit
GCCATCAATTAGTTGTCGAATAGTTTAAAAACGACCTTGTTTCTACCTGT12.272727272727273No Hit
CCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTT12.272727272727273No Hit
GCATTTTATTAAGGAAATTCCAGTCTGTGGTTAGGTATAATTGGGATTAA12.272727272727273No Hit
GATGTATGTAAAGTAATTCCATGCTTGGCATCTATAGTGTACTCAATAAA12.272727272727273No Hit
CATCGAAAATTCTCTGCCTAAGACATATAAACATCTCCCAACACCACACG12.272727272727273No Hit
TGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGT12.272727272727273No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA12.272727272727273No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA12.272727272727273No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTCTG12.272727272727273No Hit
AGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGAAGTGCTGCAA12.272727272727273No Hit
ATCAAATGTCGAAGTAACACAATAACCAAATAATAACAAACAACAATGAA12.272727272727273No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA12.272727272727273No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC12.272727272727273No Hit
GAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGACGAAAAC12.272727272727273No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA12.272727272727273No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGA12.272727272727273No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC12.272727272727273No Hit
GAGTCTTTTCTCTGCCCTCATGTGCTTGTTATTGTTTTTATCTTCATTAA12.272727272727273No Hit
AAGAAAATCTCCAAGAATACATCCAATAATACAACACCCACATAAACTTC12.272727272727273No Hit
GTATTTGTACTGTGACTGGCTCCCTCAGTTGTACCCGCGGTGCAGGACCT12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)