FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_E5_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_E5_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6726
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA681.0110020814748737No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT460.6839131727624145No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG350.5203687184061849No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC250.3716919417187035No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT210.31222123104371097No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA200.29735355337496283No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA180.2676181980374665No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA160.23788284269997023No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT160.23788284269997023No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA150.22301516503122212No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA130.19327980969372585No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG130.19327980969372585No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC130.19327980969372585No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC130.19327980969372585No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT130.19327980969372585No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT120.17841213202497772No Hit
GAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAA120.17841213202497772No Hit
ATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCAT110.16354445435622955No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA110.16354445435622955No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA110.16354445435622955No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC100.14867677668748142No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG100.14867677668748142No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC100.14867677668748142No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT100.14867677668748142No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA100.14867677668748142No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA100.14867677668748142No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA90.13380909901873325No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC90.13380909901873325No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT90.13380909901873325No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCT90.13380909901873325No Hit
CTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCA90.13380909901873325No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA90.13380909901873325No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC90.13380909901873325No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT90.13380909901873325No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG90.13380909901873325No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG90.13380909901873325No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA80.11894142134998512No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC80.11894142134998512No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG80.11894142134998512No Hit
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA80.11894142134998512No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC80.11894142134998512No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA80.11894142134998512No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA80.11894142134998512No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC70.10407374368123698No Hit
GGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTC70.10407374368123698No Hit
CAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGA70.10407374368123698No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT70.10407374368123698No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA70.10407374368123698No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG70.10407374368123698No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT70.10407374368123698No Hit
GAACGCTAATGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAAC70.10407374368123698No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA70.10407374368123698No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA70.10407374368123698No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT70.10407374368123698No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC70.10407374368123698No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC70.10407374368123698No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT70.10407374368123698No Hit
GTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGC70.10407374368123698No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG70.10407374368123698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)