FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_D4_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_D4_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC120.49382716049382713No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA110.45267489711934156No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG100.411522633744856No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT80.3292181069958848No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC70.2880658436213992No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA70.2880658436213992No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC60.24691358024691357No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC50.205761316872428No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT50.205761316872428No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA50.205761316872428No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT50.205761316872428No Hit
CTGTTTACTGTGTCCATGGTGTATCCTGTTCCTGTTCCATGGCTGTATGG50.205761316872428No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA50.205761316872428No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.205761316872428No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA50.205761316872428No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT50.205761316872428No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA40.1646090534979424No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA40.1646090534979424No Hit
GTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGA40.1646090534979424No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA40.1646090534979424No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT40.1646090534979424No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAG40.1646090534979424No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC40.1646090534979424No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT40.1646090534979424No Hit
TTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCATACACAGGCAG40.1646090534979424No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG40.1646090534979424No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG40.1646090534979424No Hit
TTGTTACATATGTGTCTTGCTCTGACATTTCAAGTCCTATTTTTAACAGG40.1646090534979424No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG40.1646090534979424No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40.1646090534979424No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA40.1646090534979424No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT40.1646090534979424No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.1646090534979424No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.1646090534979424No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA40.1646090534979424No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT40.1646090534979424No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT30.12345679012345678No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAC30.12345679012345678No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT30.12345679012345678No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.12345679012345678No Hit
TTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCT30.12345679012345678No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA30.12345679012345678No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT30.12345679012345678No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG30.12345679012345678No Hit
TGTAAATACTCATCTGCCTGTGTATGGTTAGGATTTGCATCTTTCACTTG30.12345679012345678No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA30.12345679012345678No Hit
TTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCCAAAGGG30.12345679012345678No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG30.12345679012345678No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA30.12345679012345678No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC30.12345679012345678No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC30.12345679012345678No Hit
GGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTT30.12345679012345678No Hit
ATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG30.12345679012345678No Hit
GGATACACCATGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCAT30.12345679012345678No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATGACGGATCAAGAA30.12345679012345678No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC30.12345679012345678No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGCT30.12345679012345678No Hit
GTTCTATGAAGACAACCTGACAAACAACCCAATTTTCTCTGTCCTAATTA30.12345679012345678No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG30.12345679012345678No Hit
GTAAATAGTTGTTGAATAAATGAATGAATGTCGCTTCCCTACTTCGTCTT30.12345679012345678No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC30.12345679012345678No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA30.12345679012345678No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.12345679012345678No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA30.12345679012345678No Hit
CAATTATACCTAACCACAGACTGGAATTTCCTTAATAAAATGCATTGTTG30.12345679012345678No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA30.12345679012345678No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.12345679012345678No Hit
CATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATT30.12345679012345678No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA30.12345679012345678No Hit
AATTAAGAGTTATGTTTGTGAATGGGCCAAATTAATTGATAATGGTACAA30.12345679012345678No Hit
ATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA30.12345679012345678No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA30.12345679012345678No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA30.12345679012345678No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT30.12345679012345678No Hit
AATTAGGACAGAGAAAATTGGGTTGTTTGTCAGGTTGTCTTCATAGAACA30.12345679012345678No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA30.12345679012345678No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG30.12345679012345678No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.12345679012345678No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT30.12345679012345678No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.12345679012345678No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA30.12345679012345678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)