FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACCTGTCTCTTATAC15.88235294117647No Hit
AGACAAGAGATATGGCCCAGCATTACTGAGCAATCTTGCAAAAGGAGAGA15.88235294117647No Hit
AGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCCTGTCTCTTATAC15.88235294117647No Hit
CTTGTCTTACTGATGTTTACTTTTTGATTATTGCCTGTCTCTTATACACA15.88235294117647No Hit
ACATTTCCTCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGAT15.88235294117647No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGCTGTCTCTTA15.88235294117647No Hit
TCAAAAAACCATAAACTGAACAATTAAAATTAAGAATAAACCAGTTAATC15.88235294117647No Hit
ACTACATTAAAATTTCTTTGAACTAAACAACACATAAATATATGAACTAA15.88235294117647No Hit
GCCATCAATTAGTTGTCGAATAGTTTAAAAACGACCTTGTTTCTACTCTG15.88235294117647No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA15.88235294117647No Hit
GCTCAAAGTAGCTAATAACAAATAATCATTGACTTTTGCTTGAAAATAAC15.88235294117647No Hit
ACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACA15.88235294117647No Hit
ATAAAACACAAATAAATTAAAACAACTCAACCAAAAAAATTAAAGAAAAT15.88235294117647No Hit
CATAAACGACTCCAACACCAAGTCAACCAAGCTGTAAGACTTTTACCTAT15.88235294117647No Hit
GACTGGCTGAAATTAAGTATTATAGATAACCCTATACGTTACAATACACA15.88235294117647No Hit
CTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGAGTTCTCTT15.88235294117647No Hit
CATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTT15.88235294117647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)