FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_C9_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_C9_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAATAAAATAATATACAACTAAAATAACCAAAAACTAAATCATATATAA18.333333333333332No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC18.333333333333332No Hit
TTTTTAAACTATTCTACAACAAATATATAGCCATCCGAATTCTTTTGTTC18.333333333333332No Hit
ACTAACTTGTGTACAGGTATTCCTTGTTTTTCTAAAGGAGTATTATGCCA18.333333333333332No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC18.333333333333332No Hit
CAAATGGATTCAAAGAACCAAACAAGCATGAAAATAAATCAAAAAAATGA18.333333333333332No Hit
ATTTTAAAATCTAAAAAAACTAAAAAATCACAAACAGAATAATTTACTTC18.333333333333332No Hit
GAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTAGTTTTTCTCCAG18.333333333333332No Hit
CAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG18.333333333333332No Hit
CTAATAATCAATATCAAACGACTACAAAACTCCCACATAAAATCTCTAAC18.333333333333332No Hit
AAAAAACTTAATGGTATTAAATAACTATGCTAGAATAACAAATTAGTTTC18.333333333333332No Hit
TAACATAAAAATCTAAATACTCAATACCGCACTCGAACTATAAAAACAAA18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)