FastQCFastQC Report
Tue 25 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_B4_new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_B4_new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAAC22.4096385542168677No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC22.4096385542168677No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.2048192771084338No Hit
CCTCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATT11.2048192771084338No Hit
GGGAAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT11.2048192771084338No Hit
CAATTACACCAGACAAGAGAATAATGGACAAGATACCAGAGAGGAATGAA11.2048192771084338No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGCGCCATTCCT11.2048192771084338No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC11.2048192771084338No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT11.2048192771084338No Hit
GCTCAGCTGGTTGGACTCAAGTCATGATTTCAGGGTGCTAGGATCAAGCC11.2048192771084338No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG11.2048192771084338No Hit
GGCAAGAGAAGAACCCCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGA11.2048192771084338No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCTAA11.2048192771084338No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTTAG11.2048192771084338No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT11.2048192771084338No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT11.2048192771084338No Hit
CAATGTGGAGAGAATAAAAGAACTGAGAGATCTAATGCAGTCTCTTATAC11.2048192771084338No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG11.2048192771084338No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGA11.2048192771084338No Hit
GAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAA11.2048192771084338No Hit
ATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAG11.2048192771084338No Hit
GAGAAGAACCTCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC11.2048192771084338No Hit
GGACTCTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGA11.2048192771084338No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC11.2048192771084338No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA11.2048192771084338No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA11.2048192771084338No Hit
CATTAGCGTTCTCTCCTTTTGAAAGATTGCTCAGTTCATTTATGCTTAAT11.2048192771084338No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTG11.2048192771084338No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAACTGTCTCTTATACAC11.2048192771084338No Hit
GGTGTGTTTTACGGAGGTTTATTCTCGAGTGCCTCCGAAGATAAGCTTCT11.2048192771084338No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG11.2048192771084338No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA11.2048192771084338No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA11.2048192771084338No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCTCCAGTATTCA11.2048192771084338No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT11.2048192771084338No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAACTG11.2048192771084338No Hit
GGAATGCCCTGATAAACTTCTACTGAATCTCAATCACTACTCATCCCCTC11.2048192771084338No Hit
AATGAATAGTTATTTAAAATTATGATGCAGAAAAACTGTCTCTTATACAC11.2048192771084338No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA11.2048192771084338No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGAAAAAG11.2048192771084338No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC11.2048192771084338No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA11.2048192771084338No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG11.2048192771084338No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC11.2048192771084338No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA11.2048192771084338No Hit
CATTATTACCACAGTGTCAACATCGCTTCACCTCCTTGTTTCCTGTCTCT11.2048192771084338No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTGTCTC11.2048192771084338No Hit
GTTGTGGCAATGCTACTATTTGCTATCCATACTGTCCAAAAACTGTCTCT11.2048192771084338No Hit
ATTGAAGAAATGCGGCACAGATTGAAAGCGACAGAGAATAGTTTCGAACA11.2048192771084338No Hit
GGTATCACCTCTGGCCGTAACATGGTGGAATAGGAATGGCCAAACAACAA11.2048192771084338No Hit
CTCACATGCATATTGTTACCTCACACATATCCTCTGATCTCCAGAATAAC11.2048192771084338No Hit
GGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTCTTCA11.2048192771084338No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCTGTCTCTTATAC11.2048192771084338No Hit
AGGGAAGAATATCAACAGGAACAGCAGAGTGCTGTGGATGTTGACGATGG11.2048192771084338No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT11.2048192771084338No Hit
GGGGGAGCAGCAGGCAGATCAGGCCTAGGGGGAAGCAGGCTCCCTGGTGA11.2048192771084338No Hit
CTTCTCGTCACTCCGAGCCCCACCCACTCTGAACCACGATCGGTGACCGC11.2048192771084338No Hit
CTCTTTTTTATTATAGAGGCACTGAAACTAAAGAAAATGGTGGAAGAAAG11.2048192771084338No Hit
GATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACA11.2048192771084338No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC11.2048192771084338No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTG11.2048192771084338No Hit
CACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCA11.2048192771084338No Hit
TCCCTGAACAGGGAGCCCAAAGCAGGGCTCGATCCCAGGACCCGGGGTCA11.2048192771084338No Hit
GTTTTGTTAAGGTGGCACAGGAGGTCCTTTAAACCTAATCCTCGCTGGGC11.2048192771084338No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGATAATAACCCAGCACTCAGAAT11.2048192771084338No Hit
CTTCAGATAGGTAAGTCTCCATGAATGATGTCCAAGTCAGTAGTTCAAAG11.2048192771084338No Hit
ATTATCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT11.2048192771084338No Hit
GTCGAAAGGTGCAATACTCCAGTATTCAATTACAACAAGGCAACCAAACG11.2048192771084338No Hit
ATGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCAGAC11.2048192771084338No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11.2048192771084338No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGATGTGGCCTGTCTCTTATACAC11.2048192771084338No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT11.2048192771084338No Hit
GAGATACCCAATTACAGCATACAAGAGAATAATGGACATGATTCCAGAGA11.2048192771084338No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTAGTTTCAATACC11.2048192771084338No Hit
TTGTAATTGAATACTGGAGAATTGCACCTTTCGACCTTTTCGAAATAAGT11.2048192771084338No Hit
GCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACT11.2048192771084338No Hit
TGCCAAAGGAAACAGTCTACCTGAAAGCTTGACATGGTGTTGGAGTCCAT11.2048192771084338No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCCTGTCTCTTATA11.2048192771084338No Hit
CAACAAGAGTGGCTGTTTCGATATAGAGGCCAAGGGTGTTGCCTCGTCAC11.2048192771084338No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTAGACAACTAATTGATGGCCATA11.2048192771084338No Hit
ATCATGATCTGGAAGGTGATTACACATGTAAATACTCATCTGCCTGTGTA11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)