FastQCFastQC Report
Wed 26 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_A3_new2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_A3_new2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10899
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC430.3945316084044408No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG420.3853564547206166No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT350.3211303789338471No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA280.2569043031470777No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA250.2293788420956051No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA220.2018533810441325No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA210.1926782273603083No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA190.1743279199926599No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT180.16515276630883566No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC170.15597761262501147No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT160.14680245894118726No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC160.14680245894118726No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA160.14680245894118726No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA160.14680245894118726No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC160.14680245894118726No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA160.14680245894118726No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA150.13762730525736308No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT150.13762730525736308No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC150.13762730525736308No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA140.12845215157353884No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC130.11927699788971464No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG130.11927699788971464No Hit
GTCTGATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCAT130.11927699788971464No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC130.11927699788971464No Hit
TCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA130.11927699788971464No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCTCCAGTATTCA130.11927699788971464No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA130.11927699788971464No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTGCAATTCT120.11010184420589045No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA120.11010184420589045No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT120.11010184420589045No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG120.11010184420589045No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA120.11010184420589045No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT120.11010184420589045No Hit
GGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTCCAGAG120.11010184420589045No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT120.11010184420589045No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG120.11010184420589045No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC110.10092669052206625No Hit
AGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGG110.10092669052206625No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA110.10092669052206625No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT110.10092669052206625No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT110.10092669052206625No Hit
CTATAGATGCCAAGCATGGAATTACTTTACATACATCATCTCCATTTCAC110.10092669052206625No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC110.10092669052206625No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC110.10092669052206625No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA110.10092669052206625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)