FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATCTCCAA12.380952380952381No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATCTGTCTCTTAT12.380952380952381No Hit
CCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACTCCTCAAGT12.380952380952381No Hit
GTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATTTTCCAATA12.380952380952381No Hit
GGATATGCCAAAGGAAACAGTCTACCTGAAAGCTTGACATGGTGTTGGCG12.380952380952381No Hit
ACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATCTG12.380952380952381No Hit
CACAAAAAAAATTAAGAATGCAAAAAAACAAAAAAGAGAATAAAAAATAA12.380952380952381No Hit
ATATGGGGCCCATGCAACTGGCAAGTGAACCAGTTGAATAGCTTAGTGAC12.380952380952381No Hit
GTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATCTG12.380952380952381No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT12.380952380952381No Hit
CAACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAA12.380952380952381No Hit
GGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCAT12.380952380952381No Hit
GAAAAAAACATCTTGTAAATGATGCACATGCAAATACAGAATAAACAAAT12.380952380952381No Hit
TTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT12.380952380952381No Hit
CTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCTGATCC12.380952380952381No Hit
TTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT12.380952380952381No Hit
GACCGAAAGCTGGTTAAGAGGAAATGAATAAGGAAAAATATACTCACAAT12.380952380952381No Hit
ATACTACAACTAATAAAAAAACATCAGAAAACTTTATCACGAAATAAGAC12.380952380952381No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGAATAAAGAGGATTTC12.380952380952381No Hit
CTGTACCTACTTCGCGCTACCTTTCTGACATTACCCTCGAGGAAATGTCA12.380952380952381No Hit
GACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACCTG12.380952380952381No Hit
GGAGTCCTGCTTATGTCTCTTGAGAGCCTGATCTTCTTCCATGCCTCCCT12.380952380952381No Hit
GAGCAAAAGCAGGAAAAGAATAAATGAAACAAGACTGTCTCTTATACACA12.380952380952381No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA12.380952380952381No Hit
GGCAAAGCGGGAGAGGGAAAAGCAGGCTTCCCAAAGTGCAGCTGTCTCTT12.380952380952381No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA12.380952380952381No Hit
ACCTCAGGGTAATGAACTGTACTTGTTGTTGGGCGATTCCTATGCCACCA12.380952380952381No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA12.380952380952381No Hit
CCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTCTGTCTCTTATAC12.380952380952381No Hit
CATATCTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTC12.380952380952381No Hit
GAAAAAAACCATTCACTCAATTAGAAAGAATAAAAATAACATATATTAAT12.380952380952381No Hit
GTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTGAAGTA12.380952380952381No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT12.380952380952381No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT12.380952380952381No Hit
CAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGT12.380952380952381No Hit
GGCAGAAGCTTAACAACTGAGTCACCCAGGTGTCGCTCATTTTACTTTTC12.380952380952381No Hit
TTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGT12.380952380952381No Hit
GTAAAATGAGCGACACCTGGGTGACTCAGTTGTTAAGCTGTCTCTTATAC12.380952380952381No Hit
AATTACAGCAGACAAGAGAATAATGGACATGATTCCAGCTGTCTCTTATA12.380952380952381No Hit
ACCAAAAACACACAAACAACTGCAAAATTCAAAATATCAACCAAATTACA12.380952380952381No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC12.380952380952381No Hit
CAAAAAAACAGCACATACTAAAAACAGCAAAGGACAAGGAACATTAATGA12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)