FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_A2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_A2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA36.976744186046512No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC24.651162790697675No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAACTGTCTC12.3255813953488373No Hit
GGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTAC12.3255813953488373No Hit
ATTCCAGAGAGGAATCAACAAGTACAAACCCTCTGGAGCGAAACCTGTCA12.3255813953488373No Hit
CGCTAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA12.3255813953488373No Hit
GTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATATGGCCC12.3255813953488373No Hit
CAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGGAGTCT12.3255813953488373No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT12.3255813953488373No Hit
CAGTGACAACACTGACAAGCAAGTTGATGATGCAAAAAAACAGTATTAGC12.3255813953488373No Hit
CTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAG12.3255813953488373No Hit
ATATTATCCTGCCTGAAAGAGGAAGGAAGTCCTGACACATGCTACAATGT12.3255813953488373No Hit
GTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACTGACAGCCA12.3255813953488373No Hit
ATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTA12.3255813953488373No Hit
GTCCTTAAGCATCTGCCTTTGGTTCAAGTCATGATCCCTGTCTCTTATAC12.3255813953488373No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCCACACTACGTCACCTTGCCCAAT12.3255813953488373No Hit
GTCTTGAATAAGTGGAAGGCTTCTGCCAGATTATCTAACGTCAGTTTGTT12.3255813953488373No Hit
ACTAAAAATAGGCCAGATAGGGGCACCCGGGTGGCTCAGATGGTTAAGCA12.3255813953488373No Hit
GTGTAGCAAGTGAATTAACTAACCCCTTTTAGCTTTAGTAGGCTCATCTG12.3255813953488373No Hit
TATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTGACA12.3255813953488373No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCTGT12.3255813953488373No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT12.3255813953488373No Hit
GTATACATACAATGGCATATTACTTTGTCTTCAGATGGATGGAAATCCAG12.3255813953488373No Hit
GAGCAAAAGCAGGCAAGAAAATACCACAATAATTGGTGTCCTTTGTTAAG12.3255813953488373No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA12.3255813953488373No Hit
GGCAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGC12.3255813953488373No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA12.3255813953488373No Hit
ATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGGACGTA12.3255813953488373No Hit
AAACAAATGTATTAGAAACTAAAAAAAAGGACAAAAAAAACAAGAAACAA12.3255813953488373No Hit
GCTTGGCAGGAGGCCTGCTTCTCCCTCTCCCACTCTGCCTGCTGTCTCTT12.3255813953488373No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT12.3255813953488373No Hit
ATCCAGCATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCTC12.3255813953488373No Hit
ACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATT12.3255813953488373No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTGTCTATTCTAC12.3255813953488373No Hit
CACACACACACACACACACACACACACACACACACACACACATCTCTCTT12.3255813953488373No Hit
ATCCAGATGAAGGCACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTT12.3255813953488373No Hit
GCAGAAAGCCCAACGCAGGACTTGATTGCAGGACTTGATCCCAGGACCCT12.3255813953488373No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA12.3255813953488373No Hit
ACAGAGACCTGGTCTGTGAGCGGGAGTGTGAAGACCAGCACCTGTCCGTT12.3255813953488373No Hit
AGGTGATACCATCACGCGGTCTGATCCAGCATCGTTTGTTTTGCTCCTGT12.3255813953488373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)