FastQCFastQC Report
Mon 24 Feb 2025
HT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_A10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5K2DRX5_n01_CIVIR_Ferret_Flu_Tissue_P1_A10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTCTCTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGTG12.941176470588235No Hit
CAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGAAGT12.941176470588235No Hit
GGCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA12.941176470588235No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC12.941176470588235No Hit
AATTCACTTGCTACACATACAACAGCAGGAGTTCAGATAAGCTCATACAT12.941176470588235No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC12.941176470588235No Hit
GGGACGTAGGGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT12.941176470588235No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT12.941176470588235No Hit
CCCTCTGGAGCAAAACAAACGATGCTGGATCAGACCGAGTGATGGTATCA12.941176470588235No Hit
CCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAA12.941176470588235No Hit
CCTCAGAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT12.941176470588235No Hit
CAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAG12.941176470588235No Hit
GTACTTGTTGTTGGGCCATTCCTATTCCACCATGTCTGTCTCTTATACAC12.941176470588235No Hit
TTTGACATCCTCATAAGTATGTCCTGGAAGAGACGGTAATGGTGAAATTT12.941176470588235No Hit
GCATTAAGCAAAACCCGGGGATCATTAATCAGGCACCTGTCTCTTATACA12.941176470588235No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC12.941176470588235No Hit
GTGCTATACAACAAAATGGAATTTAACCATTTCAGGCTCTTGTCCCTAAG12.941176470588235No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA12.941176470588235No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGTCAATAATCAAA12.941176470588235No Hit
GTTCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATT12.941176470588235No Hit
CCTGACAAACAACCCAATTTTCTCTGTCCTAATTAAGAGTTATGTTTGTG12.941176470588235No Hit
CAAAAGGAGAGAACGCTAATGTGCTAATTGGGCAAGGGCTGTCTCTTATA12.941176470588235No Hit
TGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCTGTCTCTTATAC12.941176470588235No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC12.941176470588235No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGCTGTCTCTTATAC12.941176470588235No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT12.941176470588235No Hit
GAGAAGAACCCCGCACTCAGAATGAATTGGATGATGCCAATGAGATACCC12.941176470588235No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT12.941176470588235No Hit
CACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAG12.941176470588235No Hit
GAGAATAATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCA12.941176470588235No Hit
CACGAATGCACTATTGAAGCACCGATTTTAGAAAAAATGAAAATTGTGCA12.941176470588235No Hit
CTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCT12.941176470588235No Hit
GCTGGGCCATATTATTCTATTGTCTGCTGTACTTGGGTATCTCATTGCCA12.941176470588235No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCCTGT12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)