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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-02-10, 14:02 based on data in: /beegfs/mk5636/logs/html/HT5CKBCX2/2


        General Statistics

        Showing 134/134 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HT5CKBCX2_l02_n01_DI222G4_P2CW_rep1
        96.3%
        45%
        4.1
        HT5CKBCX2_l02_n01_DI222G4_P2CW_rep2
        96.2%
        45%
        3.7
        HT5CKBCX2_l02_n01_DI244G4_P3CW_rep1
        95.9%
        45%
        3.5
        HT5CKBCX2_l02_n01_DI244G4_P3CW_rep2
        95.8%
        45%
        3.1
        HT5CKBCX2_l02_n01_KO_Control_M_rep1
        79.5%
        49%
        2.0
        HT5CKBCX2_l02_n01_KO_DI222_F1_rep1
        94.5%
        43%
        3.3
        HT5CKBCX2_l02_n01_KO_DI222_F1_rep2
        94.8%
        44%
        3.3
        HT5CKBCX2_l02_n01_KO_DI222_F2_rep1
        94.8%
        43%
        4.4
        HT5CKBCX2_l02_n01_KO_DI222_F2_rep2
        94.8%
        43%
        4.1
        HT5CKBCX2_l02_n01_KO_DI222_M1_rep1
        95.1%
        44%
        3.9
        HT5CKBCX2_l02_n01_KO_DI222_M1_rep2
        94.9%
        44%
        3.5
        HT5CKBCX2_l02_n01_KO_DI222_M2_rep1
        95.2%
        44%
        4.5
        HT5CKBCX2_l02_n01_KO_DI222_M2_rep2
        95.4%
        44%
        4.8
        HT5CKBCX2_l02_n01_KO_DI244_F1_rep1
        94.0%
        44%
        2.7
        HT5CKBCX2_l02_n01_KO_DI244_F1_rep2
        94.4%
        44%
        3.0
        HT5CKBCX2_l02_n01_KO_DI244_F2_rep1
        95.2%
        44%
        4.0
        HT5CKBCX2_l02_n01_KO_DI244_F2_rep2
        95.5%
        44%
        4.4
        HT5CKBCX2_l02_n01_KO_DI244_M1_rep1
        94.6%
        44%
        3.3
        HT5CKBCX2_l02_n01_KO_DI244_M1_rep2
        94.7%
        44%
        3.4
        HT5CKBCX2_l02_n01_KO_DI244_M2_rep1
        95.0%
        44%
        3.7
        HT5CKBCX2_l02_n01_KO_DI244_M2_rep2
        95.3%
        44%
        3.9
        HT5CKBCX2_l02_n01_KO_PBS_F1_rep1
        72.6%
        48%
        1.4
        HT5CKBCX2_l02_n01_KO_PBS_M1_rep1
        73.8%
        48%
        1.7
        HT5CKBCX2_l02_n01_KO_PR8_F1_rep1
        93.4%
        44%
        3.0
        HT5CKBCX2_l02_n01_KO_PR8_F1_rep2
        93.1%
        44%
        2.6
        HT5CKBCX2_l02_n01_KO_PR8_F2_rep1
        93.4%
        44%
        3.9
        HT5CKBCX2_l02_n01_KO_PR8_F2_rep2
        93.1%
        44%
        3.3
        HT5CKBCX2_l02_n01_KO_PR8_M1_rep1
        93.3%
        44%
        3.0
        HT5CKBCX2_l02_n01_KO_PR8_M1_rep2
        93.9%
        44%
        3.8
        HT5CKBCX2_l02_n01_KO_PR8_M2_rep1
        93.9%
        44%
        3.5
        HT5CKBCX2_l02_n01_KO_PR8_M2_rep2
        94.5%
        44%
        4.2
        HT5CKBCX2_l02_n01_Lib_prep_NC
        5.7%
        45%
        0.0
        HT5CKBCX2_l02_n01_MRTPCR_NC
        54.9%
        56%
        0.0
        HT5CKBCX2_l02_n01_No_index_control
        9.3%
        44%
        0.0
        HT5CKBCX2_l02_n01_PR8_P5TC_rep1
        95.3%
        45%
        3.3
        HT5CKBCX2_l02_n01_PR8_P5TC_rep2
        95.1%
        45%
        2.8
        HT5CKBCX2_l02_n01_Plasmid_control_HA
        93.7%
        47%
        2.7
        HT5CKBCX2_l02_n01_WT_Control_F_rep1
        81.3%
        49%
        2.2
        HT5CKBCX2_l02_n01_WT_DI222_F1_rep1
        94.7%
        44%
        3.8
        HT5CKBCX2_l02_n01_WT_DI222_F1_rep2
        95.2%
        44%
        4.1
        HT5CKBCX2_l02_n01_WT_DI222_F2_rep1
        94.3%
        43%
        3.3
        HT5CKBCX2_l02_n01_WT_DI222_F2_rep2
        94.8%
        44%
        3.6
        HT5CKBCX2_l02_n01_WT_DI222_M1_rep1
        94.5%
        44%
        4.0
        HT5CKBCX2_l02_n01_WT_DI222_M1_rep2
        95.0%
        44%
        4.3
        HT5CKBCX2_l02_n01_WT_DI222_M2_rep1
        94.3%
        43%
        3.4
        HT5CKBCX2_l02_n01_WT_DI222_M2_rep2
        94.9%
        44%
        3.8
        HT5CKBCX2_l02_n01_WT_DI244_F1_rep1
        95.1%
        44%
        4.4
        HT5CKBCX2_l02_n01_WT_DI244_F1_rep2
        95.1%
        45%
        3.8
        HT5CKBCX2_l02_n01_WT_DI244_F2_rep1
        95.0%
        44%
        3.8
        HT5CKBCX2_l02_n01_WT_DI244_F2_rep2
        95.3%
        44%
        4.1
        HT5CKBCX2_l02_n01_WT_DI244_M1_rep1
        94.4%
        44%
        3.3
        HT5CKBCX2_l02_n01_WT_DI244_M1_rep2
        94.9%
        44%
        3.7
        HT5CKBCX2_l02_n01_WT_DI244_M2_rep1
        95.1%
        44%
        4.4
        HT5CKBCX2_l02_n01_WT_DI244_M2_rep2
        94.6%
        44%
        3.2
        HT5CKBCX2_l02_n01_WT_PBS_F1_rep1
        75.1%
        48%
        1.9
        HT5CKBCX2_l02_n01_WT_PBS_M1_rep1
        78.6%
        48%
        2.2
        HT5CKBCX2_l02_n01_WT_PR8_F1_rep1
        89.4%
        44%
        2.6
        HT5CKBCX2_l02_n01_WT_PR8_F1_rep2
        90.3%
        45%
        2.8
        HT5CKBCX2_l02_n01_WT_PR8_F2_rep1
        93.6%
        43%
        3.2
        HT5CKBCX2_l02_n01_WT_PR8_F2_rep2
        93.1%
        43%
        2.6
        HT5CKBCX2_l02_n01_WT_PR8_M1_rep1
        93.5%
        43%
        3.4
        HT5CKBCX2_l02_n01_WT_PR8_M1_rep2
        93.7%
        44%
        3.7
        HT5CKBCX2_l02_n01_WT_PR8_M2_rep1
        93.7%
        44%
        3.9
        HT5CKBCX2_l02_n01_WT_PR8_M2_rep2
        93.5%
        44%
        3.4
        HT5CKBCX2_l02_n01_undetermined
        94.4%
        44%
        9.6
        HT5CKBCX2_l02_n01_vTC15B8B10B11_rep1_reseq
        94.5%
        44%
        2.2
        HT5CKBCX2_l02_n01_vTC15B8B10B11_rep2_reseq
        94.7%
        44%
        2.5
        HT5CKBCX2_l02_n02_DI222G4_P2CW_rep1
        95.1%
        45%
        4.1
        HT5CKBCX2_l02_n02_DI222G4_P2CW_rep2
        94.4%
        45%
        3.7
        HT5CKBCX2_l02_n02_DI244G4_P3CW_rep1
        94.5%
        45%
        3.5
        HT5CKBCX2_l02_n02_DI244G4_P3CW_rep2
        94.1%
        45%
        3.1
        HT5CKBCX2_l02_n02_KO_Control_M_rep1
        77.1%
        49%
        2.0
        HT5CKBCX2_l02_n02_KO_DI222_F1_rep1
        91.7%
        43%
        3.3
        HT5CKBCX2_l02_n02_KO_DI222_F1_rep2
        92.8%
        44%
        3.3
        HT5CKBCX2_l02_n02_KO_DI222_F2_rep1
        92.6%
        44%
        4.4
        HT5CKBCX2_l02_n02_KO_DI222_F2_rep2
        93.2%
        44%
        4.1
        HT5CKBCX2_l02_n02_KO_DI222_M1_rep1
        93.6%
        44%
        3.9
        HT5CKBCX2_l02_n02_KO_DI222_M1_rep2
        93.4%
        43%
        3.5
        HT5CKBCX2_l02_n02_KO_DI222_M2_rep1
        93.7%
        44%
        4.5
        HT5CKBCX2_l02_n02_KO_DI222_M2_rep2
        93.9%
        44%
        4.8
        HT5CKBCX2_l02_n02_KO_DI244_F1_rep1
        89.7%
        44%
        2.7
        HT5CKBCX2_l02_n02_KO_DI244_F1_rep2
        91.5%
        44%
        3.0
        HT5CKBCX2_l02_n02_KO_DI244_F2_rep1
        92.9%
        44%
        4.0
        HT5CKBCX2_l02_n02_KO_DI244_F2_rep2
        94.0%
        44%
        4.4
        HT5CKBCX2_l02_n02_KO_DI244_M1_rep1
        91.6%
        44%
        3.3
        HT5CKBCX2_l02_n02_KO_DI244_M1_rep2
        92.7%
        44%
        3.4
        HT5CKBCX2_l02_n02_KO_DI244_M2_rep1
        92.7%
        44%
        3.7
        HT5CKBCX2_l02_n02_KO_DI244_M2_rep2
        93.9%
        44%
        3.9
        HT5CKBCX2_l02_n02_KO_PBS_F1_rep1
        68.2%
        48%
        1.4
        HT5CKBCX2_l02_n02_KO_PBS_M1_rep1
        70.7%
        48%
        1.7
        HT5CKBCX2_l02_n02_KO_PR8_F1_rep1
        90.1%
        44%
        3.0
        HT5CKBCX2_l02_n02_KO_PR8_F1_rep2
        90.8%
        44%
        2.6
        HT5CKBCX2_l02_n02_KO_PR8_F2_rep1
        90.9%
        44%
        3.9
        HT5CKBCX2_l02_n02_KO_PR8_F2_rep2
        91.1%
        44%
        3.3
        HT5CKBCX2_l02_n02_KO_PR8_M1_rep1
        91.1%
        44%
        3.0
        HT5CKBCX2_l02_n02_KO_PR8_M1_rep2
        92.0%
        44%
        3.8
        HT5CKBCX2_l02_n02_KO_PR8_M2_rep1
        91.9%
        44%
        3.5
        HT5CKBCX2_l02_n02_KO_PR8_M2_rep2
        93.2%
        44%
        4.2
        HT5CKBCX2_l02_n02_Lib_prep_NC
        0.7%
        45%
        0.0
        HT5CKBCX2_l02_n02_MRTPCR_NC
        49.8%
        56%
        0.0
        HT5CKBCX2_l02_n02_No_index_control
        1.9%
        44%
        0.0
        HT5CKBCX2_l02_n02_PR8_P5TC_rep1
        92.9%
        45%
        3.3
        HT5CKBCX2_l02_n02_PR8_P5TC_rep2
        92.3%
        45%
        2.8
        HT5CKBCX2_l02_n02_Plasmid_control_HA
        90.9%
        47%
        2.7
        HT5CKBCX2_l02_n02_WT_Control_F_rep1
        78.8%
        49%
        2.2
        HT5CKBCX2_l02_n02_WT_DI222_F1_rep1
        93.1%
        44%
        3.8
        HT5CKBCX2_l02_n02_WT_DI222_F1_rep2
        94.2%
        44%
        4.1
        HT5CKBCX2_l02_n02_WT_DI222_F2_rep1
        91.4%
        44%
        3.3
        HT5CKBCX2_l02_n02_WT_DI222_F2_rep2
        93.0%
        44%
        3.6
        HT5CKBCX2_l02_n02_WT_DI222_M1_rep1
        92.4%
        43%
        4.0
        HT5CKBCX2_l02_n02_WT_DI222_M1_rep2
        93.4%
        44%
        4.3
        HT5CKBCX2_l02_n02_WT_DI222_M2_rep1
        91.7%
        43%
        3.4
        HT5CKBCX2_l02_n02_WT_DI222_M2_rep2
        93.4%
        44%
        3.8
        HT5CKBCX2_l02_n02_WT_DI244_F1_rep1
        92.9%
        44%
        4.4
        HT5CKBCX2_l02_n02_WT_DI244_F1_rep2
        93.7%
        45%
        3.8
        HT5CKBCX2_l02_n02_WT_DI244_F2_rep1
        92.3%
        44%
        3.8
        HT5CKBCX2_l02_n02_WT_DI244_F2_rep2
        93.2%
        44%
        4.1
        HT5CKBCX2_l02_n02_WT_DI244_M1_rep1
        90.8%
        44%
        3.3
        HT5CKBCX2_l02_n02_WT_DI244_M1_rep2
        93.0%
        44%
        3.7
        HT5CKBCX2_l02_n02_WT_DI244_M2_rep1
        92.6%
        44%
        4.4
        HT5CKBCX2_l02_n02_WT_DI244_M2_rep2
        92.4%
        44%
        3.2
        HT5CKBCX2_l02_n02_WT_PBS_F1_rep1
        72.7%
        48%
        1.9
        HT5CKBCX2_l02_n02_WT_PBS_M1_rep1
        75.5%
        48%
        2.2
        HT5CKBCX2_l02_n02_WT_PR8_F1_rep1
        87.1%
        44%
        2.6
        HT5CKBCX2_l02_n02_WT_PR8_F1_rep2
        88.8%
        45%
        2.8
        HT5CKBCX2_l02_n02_WT_PR8_F2_rep1
        90.5%
        44%
        3.2
        HT5CKBCX2_l02_n02_WT_PR8_F2_rep2
        89.9%
        44%
        2.6
        HT5CKBCX2_l02_n02_WT_PR8_M1_rep1
        90.6%
        43%
        3.4
        HT5CKBCX2_l02_n02_WT_PR8_M1_rep2
        91.7%
        43%
        3.7
        HT5CKBCX2_l02_n02_WT_PR8_M2_rep1
        91.4%
        44%
        3.9
        HT5CKBCX2_l02_n02_WT_PR8_M2_rep2
        91.4%
        44%
        3.4
        HT5CKBCX2_l02_n02_undetermined
        91.2%
        44%
        9.6
        HT5CKBCX2_l02_n02_vTC15B8B10B11_rep1_reseq
        90.4%
        44%
        2.2
        HT5CKBCX2_l02_n02_vTC15B8B10B11_rep2_reseq
        90.9%
        44%
        2.5

        Lane 2 Demultiplexing Report

        Lane 2 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 67/67 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        9,550,223
        4.3
        KO_PR8_M1_rep1
        3,012,314
        1.3
        KO_PR8_M2_rep1
        3,499,815
        1.6
        KO_PR8_F1_rep1
        2,982,302
        1.3
        KO_PR8_F2_rep1
        3,945,781
        1.8
        WT_PR8_M1_rep1
        3,414,124
        1.5
        WT_PR8_M2_rep1
        3,866,626
        1.7
        WT_PR8_F1_rep1
        2,619,586
        1.2
        WT_PR8_F2_rep1
        3,240,770
        1.4
        KO_DI222_M1_rep1
        3,934,033
        1.8
        KO_DI222_M2_rep1
        4,523,545
        2.0
        KO_DI222_F1_rep1
        3,277,870
        1.5
        KO_DI222_F2_rep1
        4,440,034
        2.0
        WT_DI222_M1_rep1
        3,976,500
        1.8
        WT_DI222_M2_rep1
        3,399,633
        1.5
        WT_DI222_F1_rep1
        3,773,506
        1.7
        WT_DI222_F2_rep1
        3,272,543
        1.5
        KO_DI244_M1_rep1
        3,325,174
        1.5
        KO_DI244_M2_rep1
        3,652,429
        1.6
        KO_DI244_F1_rep1
        2,672,020
        1.2
        KO_DI244_F2_rep1
        4,023,559
        1.8
        WT_DI244_M1_rep1
        3,344,151
        1.5
        WT_DI244_M2_rep1
        4,442,753
        2.0
        WT_DI244_F1_rep1
        4,388,231
        2.0
        WT_DI244_F2_rep1
        3,833,309
        1.7
        KO_PBS_M1_rep1
        1,707,307
        0.8
        KO_PBS_F1_rep1
        1,399,090
        0.6
        WT_PBS_M1_rep1
        2,206,854
        1.0
        WT_PBS_F1_rep1
        1,896,019
        0.8
        KO_Control_M_rep1
        2,045,085
        0.9
        WT_Control_F_rep1
        2,229,279
        1.0
        PR8_P5TC_rep1
        3,343,791
        1.5
        DI222G4_P2CW_rep1
        4,119,949
        1.8
        DI244G4_P3CW_rep1
        3,523,643
        1.6
        MRTPCR_NC
        7,217
        0.0
        KO_PR8_M1_rep2
        3,752,591
        1.7
        KO_PR8_M2_rep2
        4,210,919
        1.9
        KO_PR8_F1_rep2
        2,623,195
        1.2
        KO_PR8_F2_rep2
        3,337,981
        1.5
        WT_PR8_M1_rep2
        3,701,887
        1.7
        WT_PR8_M2_rep2
        3,418,441
        1.5
        WT_PR8_F1_rep2
        2,758,245
        1.2
        WT_PR8_F2_rep2
        2,618,759
        1.2
        KO_DI222_M1_rep2
        3,524,499
        1.6
        KO_DI222_M2_rep2
        4,814,534
        2.2
        KO_DI222_F1_rep2
        3,305,931
        1.5
        KO_DI222_F2_rep2
        4,100,316
        1.8
        WT_DI222_M1_rep2
        4,295,131
        1.9
        WT_DI222_M2_rep2
        3,828,004
        1.7
        WT_DI222_F1_rep2
        4,139,225
        1.9
        WT_DI222_F2_rep2
        3,602,471
        1.6
        KO_DI244_M1_rep2
        3,407,658
        1.5
        KO_DI244_M2_rep2
        3,899,921
        1.7
        KO_DI244_F1_rep2
        3,035,154
        1.4
        KO_DI244_F2_rep2
        4,402,525
        2.0
        WT_DI244_M1_rep2
        3,715,914
        1.7
        WT_DI244_M2_rep2
        3,185,243
        1.4
        WT_DI244_F1_rep2
        3,783,405
        1.7
        WT_DI244_F2_rep2
        4,117,873
        1.8
        PR8_P5TC_rep2
        2,768,626
        1.2
        DI222G4_P2CW_rep2
        3,672,982
        1.6
        DI244G4_P3CW_rep2
        3,116,767
        1.4
        Plasmid_control_HA
        2,704,973
        1.2
        Lib_prep_NC
        141.0
        0.0
        vTC15B8B10B11_rep1_reseq
        2,207,093
        1.0
        vTC15B8B10B11_rep2_reseq
        2,541,975
        1.1
        No_index_control
        107.0
        0.0

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        CTCTCTACTCTTTCCC
        106478.0
        1.1
        CGTACTAGTCTTTCCC
        58272.0
        0.6
        TAAGGCGATCTTTCCC
        58116.0
        0.6
        AGGCAGAATCTTTCCC
        57760.0
        0.6
        CAGAGAGGTCTTTCCC
        52602.0
        0.6
        TAGGCATGTCTTTCCC
        46292.0
        0.5
        GGACTCCTTCTTTCCC
        44567.0
        0.5
        CTCTCTCCTCTTTCCC
        26909.0
        0.3
        TCCTGAGCTCTTTCCC
        20617.0
        0.2
        CGAGGCTGTCTTTCCC
        19110.0
        0.2
        GGATCCTAAAGGAGTA
        17804.0
        0.2
        GCACTCCTTCTTTCCC
        16522.0
        0.2
        GGATCCTAACTGCATA
        14381.0
        0.1
        GGATCCTACTAAGCCT
        14366.0
        0.1
        CTCTCTACTGCATATC
        13792.0
        0.1
        GTAAGGAAACTGCATA
        13166.0
        0.1
        CGTACTAGTGCATATC
        12381.0
        0.1
        TAGGCATGTGCATATC
        11958.0
        0.1
        AGGCAGCATCTTTCCC
        11884.0
        0.1
        CAGAGAGGTGCATATC
        11434.0
        0.1

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        2.0
        247,273,360
        223,481,551
        4.3
        2.4

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (151bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).