FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_vTC15B8B10B11_rep1_reseq.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_vTC15B8B10B11_rep1_reseq.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207093
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG92320.41828776585309274No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA81070.36731574065977285No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT59260.26849797448498997No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA51120.23161688247844564No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT43320.1962762783444105No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG40670.18426953463220624No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT39580.17933091174680904No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT37860.1715378554505859No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT37770.17113007924903934No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC37600.17035983531278476No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA33920.15368631951621434No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA31340.1419967350718796No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT30260.1371034206533209No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC29150.13207418083424668No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG28640.12976344902548284No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT28520.12921974742342077No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT28430.1288119712218742No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA27620.12514198540795518No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA26780.1213360741935206No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG26500.12006743712204243No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG25660.11626152590760788No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT25620.11608029204025386No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC25110.11376956023149001No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT24940.11299931629523541No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG24190.10960118128234742No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG24070.10905747968028533No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA23460.10629366320313643No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA23420.1061124293357824No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT23040.10439070759591916No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT22520.10203466732031682No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA22440.10167219958560876No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA22190.10053948791464609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8850.0133.6051
GCAAAAG52450.0103.865853
AGCAGGC5350.092.165028
GCAGGGT33100.083.46579
GCAGGGG13650.083.4023069
AGCAAAA67550.081.2922062
AGCAGGG49900.077.888858
GTCAATT7550.076.863722
CCTGTTT1350.075.17568145
AGCGAAA18300.072.936531
ACGGCCC608.043207E-872.49084145
AAGCAGG92400.071.7274257
CAAAAGC79750.068.492444
TGACGCC2800.067.31292145
AAGGGTG9600.062.7169955
ACTATCG350.00355926162.1772581
AGGGTGT9750.060.984616
TCAATTA9750.059.5201153
ACAAGGG11750.058.649473
CAAGGGT11900.057.3006134
Produced by FastQC (version 0.11.5)