FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9550223
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG312360.32707089666911443No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA242210.2536171144904156No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA207150.21690592984059115No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA146010.15288648233659047No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT137290.14375580549270944No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA137190.14365109589587594No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC127310.13330578772872634No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT123920.12975613239607076No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT114650.12004955276960548No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT114620.12001813989055544No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT110660.11587163985594892No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC102190.10700273700415164No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT101700.10648965997966749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA30000.0121.65471
GCAAAAG174950.099.743213
GAGCAAA209000.084.4999541
GCAGGTC119700.079.002419
AGCAAAA222750.078.9581762
CAAAAGC224250.078.30064
AGCAGGC32500.074.3049558
AAAAGCA258600.067.9565
GCAGGGT29250.064.9579549
AGCAGGG86900.064.2580958
TAAACTA86450.059.5063635
AAGCAGG363400.058.389227
AAAGCAG365300.057.9465686
CTATTCG88400.056.9330069
CGAAAGC71200.054.927643
TTTTTAA95400.053.543461
TTTAAAC97400.052.369523
GCAGGCA45250.044.8741659
AGCAGGT298900.043.914678
CAAGGGT17800.043.6160134
Produced by FastQC (version 0.11.5)