FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_WT_PR8_F1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_WT_PR8_F1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758245
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT218910.793656836140372No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT139670.5063727116336656No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA92940.3369533888396426No Hit
CCATAGCCTCCACCATACTGGATATTCCGACTGGTCTTCTGTATGAACTG79420.2879367133811536No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG70620.2560323684081726No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA69500.2519718154116114No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG69290.25121046172475614No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT68180.2471861636656642No Hit
GGACATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCTG66800.24218298229490126No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG66150.239826411359397No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA65860.23877501817278743No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA63480.23014634305509482No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG60910.2208288241254856No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC60820.22050252968826195No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT60750.22024874512597686No Hit
GGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACT59360.2152093088177446No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT58230.21111250088371408No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA56670.2054567306385038No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT53840.1951965833346929No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA48920.17735915409979897No Hit
ATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAA48870.1771778794124525No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA48330.17522011278911048No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC47720.17300856160348338No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG45910.1664464179215407No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT45000.1631472186118347No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG44450.16115319705102338No Hit
GCTTTTCAAAAATACCAGGATGGGATTCCTCAAGGAAAGCCATTGCTTCC44200.160246823614291No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG41490.1504217355601116No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT40960.14850022387423886No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG40420.14654245725089685No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA39260.14233688450445847No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT39160.14197433512976548No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC39070.14164804069254183No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA37680.13660860438430958No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA37300.1352309167604763No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA37230.13497713219819124No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA36110.13091657920163002No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC33900.12290423802091548No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA32140.11652336902631927No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA32040.11616081965162631No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA31990.11597954496427981No Hit
TCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTG31920.11572576040199474No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC31240.11326042465408256No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA31150.1129341302168589No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT30370.11010624509425378No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG30100.10912736178258277No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT30040.10890983215776698No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC30010.1088010673453591No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG29760.10789469390862667No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC29640.1074596346589951No Hit
ATACTGGATATTCCGACTGGTCTTCTGTATGAACTGCTTTTCAAAAATAC29030.10524808347336803No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT28660.10390665078700405No Hit
CTTCCAATACACAATCTGTTTGGGCATAACCACTTGGTTCATTGTCTTCT28490.10329031685002601No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG27860.10100625578946033No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC27620.1001361372901972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16050.0130.183071
GCAAAAG86100.099.513923
AGCAGGC48900.094.91338
CTTCACG259.3628606E-487.059931
AGTAGAA36450.081.606251
CAAAAGC106100.080.6870354
GAGCAAA109050.078.371241
GCAGGCA56050.078.01859
AAGCAGG137350.077.50787
AGCGAAA29100.071.303382
AGCAAAA123450.069.64092
CTATTCG18600.067.061229
TAAACTA18950.065.084385
GGCGCCC702.3381108E-762.13494145
CGATACC350.003568968762.13494145
GTAGAAA48650.061.738292
TCTCGGG8700.061.658813145
GCAGGGG27800.058.693999
TAGAAAC51350.058.492073
GCAGGGT11550.057.7645959
Produced by FastQC (version 0.11.5)