FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_WT_PR8_F1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_WT_PR8_F1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619586
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT213460.8148615849985454No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT95000.3626527245144844No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA87080.3324189394812768No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT84870.3239824918899398No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA69440.2650800546345873No Hit
CCATAGCCTCCACCATACTGGATATTCCGACTGGTCTTCTGTATGAACTG66760.2548494304061787No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT64620.24668020061185242No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA60090.22938739174816172No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA58970.22511190699599096No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA56640.21621737175263572No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA54680.20873527343633688No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG53230.20320004764111582No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG52330.19976439025097859No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC51850.19793203964290543No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG51170.19533620961480172No Hit
GGACATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCTG50410.19243498781868587No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG50160.19148063854364775No Hit
ATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAA50000.19086985500762335No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT49590.18930472219656083No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG49080.18735784967548308No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG48830.18640350040044495No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT43580.1663621656246445No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT43070.16441529310356676No Hit
GGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACT42150.16090328777142648No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT41300.15765850023629688No Hit
GCTTTTCAAAAATACCAGGATGGGATTCCTCAAGGAAAGCCATTGCTTCC41210.15731493449728315No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA38680.14765691983389742No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA38600.14735152806588522No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG36330.13868603664853912No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA35230.13448689983837142No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA34980.1335325505633333No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT34400.13131846024524485No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA33120.12643219195704972No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA32410.12372184001594146No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC32080.12246209897289113No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC32020.12223305514688199No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG31650.12082061821982557No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC30780.11749948274269292No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA30500.11643061155465025No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC30210.11532356639560604No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG29960.11436921712056791No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG29020.11078086384642459No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC28760.10978834060038495No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG28430.10852859955733463No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC28280.10795598999231176No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG28270.10791781602131024No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA27860.10635268321024771No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA27040.10322241758812271No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG26230.1001303259369992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA17900.0127.673011
GCAAAAG101400.0101.456483
AGCAGGC45900.099.05418
GAGCAAA120650.085.8701861
CAAAAGC122150.084.459344
AAGCAGG156850.080.164517
GCAGGCA56550.077.1936349
AGCAAAA134850.076.612672
AGTAGAA31800.076.4291841
AGCGAAA30400.074.937222
GCAGGGT14600.070.029959
AAAGCAG191850.065.842146
GCAGGGG36800.065.6166469
AGCAGGT59250.064.497058
AAAAGCA161650.064.358625
GCAGGTC27750.063.4981279
AGCAGGG69650.059.343258
CGGTGGG7950.059.268356145
GTAGAAA44300.055.190932
ATCTATG7950.054.7552382
Produced by FastQC (version 0.11.5)