FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_WT_PBS_M1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_WT_PBS_M1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206854
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG153990.6977806415829955No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA78540.3558912370279139No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG60600.27459904461282897No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG47620.2157822855521933No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT46150.2091212196185158No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG39760.18016597382518282No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA38760.17563463645533414No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA37930.17187362643835977No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA36930.16734228906851112No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC36200.16403441278852157No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG34770.157554600349638No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC33360.15116541465815136No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC32370.1466793906620012No Hit
GAGCAAAAGCAGGCAAAAGAGTCAGAGACAGTCCCACTCCCACTGTTAGG31790.14405121498748896No Hit
AGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG31660.14346214112940867No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG30130.13652919495354018No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA29500.13367445241053555No Hit
AGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG29170.13217911107848548No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC28990.13136347035191273No Hit
TCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCT28770.130366576130546No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG27940.12660556611357163No Hit
CCTTATCTCCCTCCATGTGTCTTCCCCTCCCTCACTCTCAGCCCCATCTT25490.11550378955744241No Hit
GATATAAACAGGGGAGATATAAACAAGGGAGATATAGACTGAATAAATGT24020.10884272362376488No Hit
GAGCAAAAGCAGGAGGGATCAAGTAAGAGGGAGATGGAGGGAGAAATTAT23530.10662236831253903No Hit
GAGCAAAAGCAGGCAAAAGTGTCAGAGACAGCCTCTGCTCCCGCTGTTAG22750.10308792516405707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA118900.0106.22861
AGCAAAA121200.0103.853552
GAGCGAA27000.0101.029591
CAAAAGC136250.092.914484
CGCGAAA259.363689E-487.057412
GCAAAAG148250.085.344653
AAAAGCA160350.079.176015
GCGCGAA300.001930992872.5478441
AAAGCAG230350.067.020126
TGACGCC3700.066.614235145
AAGCAGG246000.063.1985977
AGCGAAA43550.062.4694442
GCGAAAG45800.059.7173273
ACGAGCC2100.058.683968145
CGAAAGC47400.058.0076644
AGCAGGC85150.057.4839678
GCAGGCC27150.053.685119
GCCGACC2650.046.504276145
AGCAGGG158000.044.8399438
GCAGGCA64200.043.2604839
Produced by FastQC (version 0.11.5)