FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_WT_PBS_F1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_WT_PBS_F1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896019
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG93880.49514271745167115No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA55910.29488101121349525No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG46600.2457781277508295No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG40900.21571513787572802No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG34600.1824876227506159No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT31420.1657156389255593No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA29460.1553781897755244No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA28080.14809978170049984No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC26630.14045217901297402No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC25280.13333199720045No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA25060.13217167127544607No Hit
TCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCT24860.1311168295254425No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC23910.1261063312129256No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG22690.1196717965379039No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG21410.1129208093378811No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA20170.10638079048785903No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC20090.1059588537878576No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG19420.10242513392534568No Hit
CCTTATCTCCCTCCATGTGTCTTCCCCTCCCTCACTCTCAGCCCCATCTT19220.10137029217534212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA81900.095.58431
AGCAAAA83100.093.330952
GAGCGAA19000.091.2626951
GCAAAAG91900.084.630763
CAAAAGC94650.082.555124
AAAAGCA120650.064.9432455
AGCAGGC54750.060.7954758
AGCGAAA30400.059.425761
AAAGCAG163150.058.22586
GCGAAAG32150.056.1910742
GCAGGCC22400.056.00689
AAGCAGG174150.054.7562267
CGAAAGC34100.054.041613
TGACGCC11300.048.11203145
GCCGACC2350.043.18481145
AGCAGGG121450.036.2438138
TCGCCGG9200.035.45647145
GCAGGCA42400.034.548429
CGGTGGG9200.033.880627145
GACGAAA3900.033.456367145
Produced by FastQC (version 0.11.5)