FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_WT_DI244_F1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_WT_DI244_F1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3783405
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA678631.7937017052099893No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG578351.5286494573010292No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA361090.9544048284547915No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA287810.7607168674778407No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC274510.7255633483594804No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC270100.7139071814939189No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT259260.685255741851586No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC229140.6056449150963219No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG220000.5814867824089676No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG219180.579319422583625No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT203360.5375052366849439No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT200680.5304216704265073No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG190740.5041490403485749No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT182510.48239614844300305No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT177030.4679118413175433No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA174640.46159478036319135No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC174050.46003533853764006No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA168900.44642326158579376No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA152600.40334037725276567No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT147330.38941112569233266No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG142930.37778139004415334No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA142550.37677700378362877No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA140060.3701956306554545No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC134830.35637210396455044No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC132280.3496321435320829No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA131760.34825772022820717No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT130440.34476879953375333No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC127760.3376852332753168No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG126140.33340337605939624No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC121720.32172077797645243No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATC102880.271924364428339No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG101770.26899049929891194No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTG92740.24512311000276207No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA92590.24472664174202868No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGCCC92520.24454162322035308No Hit
CCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG92070.2433522184381529No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC91690.24234783217762837No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC90050.2380131125269433No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA89430.23637437704924535No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT86640.22900006739960432No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA85390.22569616522682612No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG84410.22310590592336796No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT83770.22141430801090553No Hit
ACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT82880.2190619296638874No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC81960.21663025766472266No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTG80490.21274486870953546No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC75490.19952926001842258No Hit
CTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCTGTC75080.19844558010575128No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG73950.19545885254155979No Hit
GTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGGA73840.1951681091503553No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA73460.19416372288983072No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT73220.1935293736726573No Hit
CATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGA72410.19138844506469702No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT72130.1906483709779947No Hit
GTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGC71220.18824313019621214No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG68900.18211108776353574No Hit
CACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA67620.17872789193861086No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA67200.1776177808085574No Hit
TATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCT65170.17225224367996553No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTT64540.1705870769848853No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC62700.16572373298655577No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA61010.1612568572489596No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCT58800.1554155582074877No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA57740.15261384916497178No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG57660.15240239942591396No Hit
TCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGG56860.1502879020353359No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT56850.15026147081795366No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG56850.15026147081795366No Hit
GATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGC56200.14854344168810898No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG56080.14822626707952227No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA55620.14701043107993989No Hit
GTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTGCGACATTA54960.145265970732713No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC54050.14286072995093044No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA53970.14264928021187265No Hit
GGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCT53650.1418034812556414No Hit
GAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGG53580.14161846273396583No Hit
TCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACG52590.13900177221312549No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT52340.13834099177856982No Hit
ACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGGATGTG51260.13548642030128943No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT51030.13487850230149825No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA51000.13479920864935158No Hit
ATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAG50940.13464062134505822No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA50870.13445560282338262No Hit
AGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGG50730.13408556578003147No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTC50390.1331869043890358No Hit
GAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTG48610.1284821476949996No Hit
GGACAAGAGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTG48580.12840285404285293No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAG48420.12797995456473732No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCG48130.12721344926065276No Hit
GGGATGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCATACTTACT47600.12581259473939482No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA46210.12213865552326542No Hit
GACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCA45840.12116070048012306No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT45170.11938980891551393No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCC45000.1189404782200161No Hit
GATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGA44800.11841185387237159No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG44490.11759248613352259No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA44080.11650880622085132No Hit
GTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTAT43660.11539869509079784No Hit
ATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTGCGA43360.11460575856933107No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA43100.1139185469173932No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC42220.11159259978775732No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA41730.11029747013602827No Hit
GGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCAT41030.10844728491927245No Hit
GGCTAATGTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGA40580.10725788013707228No Hit
CAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGA40300.10651780605036996No Hit
CCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTC40000.10572486952890321No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCT38520.10181304935633378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA25750.0132.153461
GACGTCC203.8691226E-4108.73428145
GGCGCCC352.765628E-7103.556465145
GCAAAAG154150.098.375223
CAAAAGC163850.092.5956344
GAGCAAA167650.090.843071
AGCAGGC4800.089.1382148
AGCAAAA171650.088.852942
AAAAGCA177750.085.558785
GCAGGTC159500.084.249629
AGTAGAA36250.081.8651
GGAGAAA29300.080.65387145
AGCAGGT199150.079.0191968
AAGCAGG236050.078.801887
GTCAATT3250.078.138962
GCAGGGG23400.077.4778069
AGCGAAA54600.076.677181
TACCGGA300.0019300772.55762
GCTCAAC300.001937271272.48952145
TCTCGGG11800.070.64656145
Produced by FastQC (version 0.11.5)