FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_WT_Control_F_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_WT_Control_F_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229279
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG230661.0346843082449528No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA122730.5505367430456215No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG93440.4191489714836052No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT75710.3396165307258535No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA60450.27116390546001645No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA59950.2689210278300742No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG59360.26627443222674235No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG57280.25694406128618263No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG56310.25259287868409475No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA55540.24913884713398368No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC53390.23949447332523205No Hit
TCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCT50870.2281903700703232No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC50830.2280109398599278No Hit
AGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG48910.2193982897609496No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC46660.20930534042620957No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG45150.20253184998378398No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG44730.20064783277463252No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA43480.19504063869977692No Hit
AGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG41610.18665227636379295No Hit
CCTTATCTCCCTCCATGTGTCTTCCCCTCCCTCACTCTCAGCCCCATCTT40680.1824805239721004No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC40260.18059650676294892No Hit
CCCTCACTCTCAGCCCCATCTTCCCTCCCTTTCTCTGCCTCCACACTTCC35750.16036575054086993No Hit
GGTAAAAGCTACAGTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGT32540.1459664761566408No Hit
GAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGG32000.14354416831630315No Hit
GAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCAT29330.13156720177241163No Hit
GATGTGGACCATGGTAAAAGCTACAGTAATGGGCCCTGCTCCTTTGTGAG29000.13008690253664973No Hit
GACTAAGAGTGGGGAGGGGAGGAAGTGTGGAGGCAGAGAAAGGGAGGGAA28990.13004204498405092No Hit
AAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATG28810.12923460903727169No Hit
CAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTG28380.12730573427552136No Hit
GAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGGAAGGGAAGTT27680.12416570559360222No Hit
GTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGCC26740.11994909564931083No Hit
GTATAATAACAATCTGAACGGAATCTTAGCTGATGAAATGGGGCTAGGCA26230.11766136046676974No Hit
GGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGA25980.11653992165179863No Hit
CTCTCAGCCCCATCTTCCCTCCCTTTCTCTGCCTCCACACTTCCTCCCCT25860.11600163102061249No Hit
GAGTGAGGGAGGGGAAGACACATGGAGGGAGATAAGGAGGGAGGATGGGA25760.11555305549462405No Hit
GATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGGAA25430.11407275625886218No Hit
ATGTGGACCATGGTAAAAGCTACAGTAATGGGCCCTGCTCCTTTGTGAGA25430.11407275625886218No Hit
GAGAAAGGGAGGGAAGATGGGGCTGAGAGTGAGGGAGGGGAAGACACATG25140.11277188723349567No Hit
GTTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCT24860.11151587576072802No Hit
GGTCAAGGAAGGAGGGAAGGCAAAGCTAGATAAATGACAGAAGGCAGCTC24830.1113813031029315No Hit
CTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCTCCCTCCATGT24010.10770298378982622No Hit
GAGCAAAAGCAGGCTGCACCACGCTGCACAGCGGCAAAGGATGGTGATGA23250.10429380979231401No Hit
GCCTTCCCTCCTTCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCT22750.10205093216237178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA28700.0115.255681
GAGCAAA115150.0110.81061
AGCAAAA120850.0104.8037952
CAAAAGC137750.092.209144
GCAAAAG138350.091.7568053
AAAAGCA159200.079.648535
AGCGAAA45950.070.88272
AAAGCAG236300.067.3542566
GCGAAAG48500.067.1558763
AAGCAGG249950.063.7339977
CGAAAGC51350.063.4286234
TGACGCC4350.061.66082145
AGCAGGC72850.059.3226668
GCAGGCC26650.058.7706539
AGTAGAA25400.058.546311
GCTCGGA553.211038E-452.7565882
GCAGGGA131200.048.469639
AGCAGGT24450.046.5612648
GTAGAAA32900.045.1998862
AGCAGGG199750.045.0493978
Produced by FastQC (version 0.11.5)