FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_PR8_P5TC_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_PR8_P5TC_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768626
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG178460.6445796579241834No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT112810.4074584288379868No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC107630.3887487873046052No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA107180.3871234323451416No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT82870.29931814553500546No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA78280.28273952494847626No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA69890.25243568470425404No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC66120.23881882204385857No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT64150.2317033792213177No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT62100.22429898440598334No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC61840.22335989042940432No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT57320.20703410283656945No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT56370.20360279792214622No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA55970.20215803795817855No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA53920.19475364314284413No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA53760.19417573915725708No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA52930.19117786223202413No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT50680.18305108743470588No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT48760.17611623960766098No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC48290.17441864664999895No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT45980.16607515785808555No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG45800.1654250158743001No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA45100.16289668593735665No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT44420.16044059399861157No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA43920.15863464404365196No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA43380.1566842180922956No Hit
GAATGGACCAGGCGATCATGGATAAGAACATCATACTGAAAGCGAACTTC43310.15643138509860124No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC40910.14776282531479512No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG40860.14758223031929918No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT40520.14635418434992664No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAACATCATACTGAA40380.14584851836253795No Hit
GATAAGAACATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCT40110.14487330538685977No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT40040.14462047239316542No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA39740.14353690242018965No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA38930.1406112634931551No Hit
GTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTGCCA38190.13793845755981488No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG38040.137396672573327No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC37910.1369271255850375No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC37420.13515729462917708No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT37190.13432655764989565No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG37150.13418208165349887No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT36910.13331522567511828No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC36320.13118420472826592No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA36190.13071465773997645No Hit
GGTACAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAG35570.1284752797958265No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC35500.12822244680213218No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG35430.12796961380843783No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA34540.12475502288860972No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA34120.12323802492644365No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC33790.12204609795617033No Hit
CTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA33780.12200997895707112No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGAACT33760.12193774095887273No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT32840.11861479304174705No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA32040.1157252731138117No Hit
CTTAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACAT31990.11554467811831573No Hit
GTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCTTC31560.11399156115705046No Hit
CTTCCAGGACATACTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCAT30720.11095756523271831No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT30670.11077697023722236No Hit
GTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGAA30220.10915161527775871No Hit
CTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCT30060.10857371129217165No Hit
CCCTAAGAGGAAGGGGCAGCACCCTCGGTCTGGACATCGAGACAGCCACA29910.10803192630568376No Hit
GTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTT29830.10774297431289022No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC29790.10759849831649346No Hit
CTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAAT29580.10683999933541041No Hit
GCATGGACCAGTCCCTTGACATTTCCTCAAGAGTCATGTCAGTTAGGTAA29500.10655104734261688No Hit
GTATGATGTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGG29290.10579254836153384No Hit
AAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGT29240.10561195336603788No Hit
ACCTAATTGTTCCCGCCATTTCTCGTTTCTGTTTTGGAGTGAGTGGAGGT28810.10405883640477262No Hit
CTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTCTGTTTTGGAGTGAGT28680.10358928941648313No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA28520.10301138543089604No Hit
ATGATGTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCC28340.10236124344711059No Hit
GTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCTCT28310.10225288644981302No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT28280.10214452945251544No Hit
GCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATTTCACCAT28250.10203617245521787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9200.0136.422331
AGCAAAA56800.095.28352
GCAAAAG56400.095.1874853
AGCGAAA22050.083.5703661
ACTAATA3050.080.836988
AGCAGGC3850.073.457298
AGCAGGG66950.071.81158
GCAGGGT57800.071.511859
TACTAAT3450.071.464587
CTACTAA3500.070.443666
TGACGCC3650.067.52553145
GCAGGGG9800.067.3358469
CCTGTTT1900.064.860054145
AAGGGTG29700.064.487385
ACAAGGG33450.064.19823
CTATTCG2500.063.8136679
CAAGGGT34350.062.727354
GGTGGGG350.003568993662.134842145
CAAAAGC87850.061.8539284
TTAGTAG3300.061.556131
Produced by FastQC (version 0.11.5)