FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_No_index_control.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_No_index_control.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT21.8691588785046727No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG21.8691588785046727No Hit
GGCAAAGTGATGTGTGTGTGCAGAGACAACTGGCATGGTTCGAACCGGCC10.9345794392523363No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA10.9345794392523363No Hit
ATGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCATACTTACTGAC10.9345794392523363No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG10.9345794392523363No Hit
CATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGA10.9345794392523363No Hit
ATGCTAGACCATAGTTCCCGTCTTATTACCACCACCTCATCACCCTCCCC10.9345794392523363No Hit
AACTGTGACTTTTGGTCCTTCCTATCCAAACACCATTACCATACCTATAT10.9345794392523363No Hit
TTCGTAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTT10.9345794392523363No Hit
AGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTG10.9345794392523363No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA10.9345794392523363No Hit
CACTTGACATTGTGGATTCTTGATCGTCTTTTTTTCAAATGCATTTACCG10.9345794392523363No Hit
TGCTGGGAGTCAGCAATCTGTTCACAGGTTGCACATACCAGGCCAAATGC10.9345794392523363No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT10.9345794392523363No Hit
GAATAACTGAGTGAGATTTCTGTGGCCCCATGGAATGTTATCTCCCTCTT10.9345794392523363No Hit
GATTTTCACTTCATCAATGAGCAAGGCGAGTCAATAATCGTAGAACTTGG10.9345794392523363No Hit
CCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGAACTGTGACTTTT10.9345794392523363No Hit
TTCTTATGCTATTGTCTTTGCTGTATATAGCCCACCCACGGATGGGACAA10.9345794392523363No Hit
GTTTACCACGTCGGTGTCATTCCTTAAGTGGGATCTTCCTTTTATGATGA10.9345794392523363No Hit
GACATGCACTTGCTGACCAAGCAACCGATTCAAATCTTGAATTGTACGGG10.9345794392523363No Hit
AGCGAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATT10.9345794392523363No Hit
GTTGCTTGGTCAGCAAGTGCATGTCATGATGGCATGGGCTGGCTAACAAT10.9345794392523363No Hit
GTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGAT10.9345794392523363No Hit
ATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTACTCTCTATC10.9345794392523363No Hit
ACACATCAGTACTCAGAAAAGGGAAGATGGACAACACTTGAAGATGAACA10.9345794392523363No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA10.9345794392523363No Hit
AAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGC10.9345794392523363No Hit
TGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACTGGAGACCCT10.9345794392523363No Hit
CCAAGAGATTTCATCGACTATGAGGAGCTGAGGGAGCAATTGAGCTCAGT10.9345794392523363No Hit
CTGCTGCTTGCTCACTCGATCCAGCCATTTGCTCCATAGCCTTAGCTGTA10.9345794392523363No Hit
CTCTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGT10.9345794392523363No Hit
GTGGCAGGCCCTCTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAA10.9345794392523363No Hit
AAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCTTTC10.9345794392523363No Hit
TCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTT10.9345794392523363No Hit
ATGTTTTACTATAATGACTGATGGCCCGAGTGATGGGCTGGCCTCGTACA10.9345794392523363No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAAAAGACTGAGGGGATTCCTCA10.9345794392523363No Hit
TCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGG10.9345794392523363No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC10.9345794392523363No Hit
GTGCCTTACTAAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGC10.9345794392523363No Hit
CCATGGAGGTTGCTAGTCAGGCTAGACAAATGGTGCAAGCGATGAGAACC10.9345794392523363No Hit
TTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATA10.9345794392523363No Hit
AACAGGAACATTCCTCATAGTGAGAATTAGGTGCATTCAACTCTATTGAT10.9345794392523363No Hit
AATCAATAGAGTTGAATGCACCTAATTCTCACTATGAGGAATGTTCCTGT10.9345794392523363No Hit
ACAAAAAGAAATGCTGCTCGCACTAGTCCAGATTGTTTTTTCTTTAGGTC10.9345794392523363No Hit
CTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCTGTCTT10.9345794392523363No Hit
GGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATCTGAAGCA10.9345794392523363No Hit
TCCTCTCGCTATTGCCGCAAATATCATTGGGATCTTGCACTTGACATTGT10.9345794392523363No Hit
TGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAAT10.9345794392523363No Hit
CCATAACTCGGGCCATCAGTCATTATAGTAAAACATGAACCATTTACACA10.9345794392523363No Hit
TGCTAATCCATATTGAGATTATATTCCCTATTTGCAATATTAGGCTAATT10.9345794392523363No Hit
TAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGC10.9345794392523363No Hit
GTATGGTTACAAGGAGTATAGTTTCATCGAATTTGGAGTAACAAGGAGAG10.9345794392523363No Hit
AGCGAGAGGATCACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCTGA10.9345794392523363No Hit
ACTCGGTTCTTCTGGATGTTAATTTCTTGATTATGCCCTTATGGTCAAGG10.9345794392523363No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA10.9345794392523363No Hit
GTAGAACATGAACCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAA10.9345794392523363No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCT10.9345794392523363No Hit
TAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTA10.9345794392523363No Hit
AAAAAGAAATGCTGCTCGCACTAGTCCAGATTGTTTTTTCTTTAGGTCGT10.9345794392523363No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTAGCAGTCTCGCACCCGCG10.9345794392523363No Hit
ATCTTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCC10.9345794392523363No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG10.9345794392523363No Hit
GAGCAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG10.9345794392523363No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT10.9345794392523363No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG10.9345794392523363No Hit
CTATTGATTTAGTAACCTTCCCCTTTTCGATCTTGAAAATTTTGTACGAG10.9345794392523363No Hit
GTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGAC10.9345794392523363No Hit
ACCTATAAAAATAGCACCTGGGTAAAGGACACAACTTCAGTGATATTAAC10.9345794392523363No Hit
ATAGAGTTGAATGCACCTAAGTCTCACTATGAGGAATGTCCCTGTTACCC10.9345794392523363No Hit
CTCTTATGACAAAAACGTCTCCTTTGGAACCAATTCTTATGCTATTGTCT10.9345794392523363No Hit
CAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGA10.9345794392523363No Hit
CTTAGGGACAACCTTGAACCTGGGAGCTTTGATCTTGGGGGGCTATATGA10.9345794392523363No Hit
ACCCAGAAGCACGGCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTG10.9345794392523363No Hit
AACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGT10.9345794392523363No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC10.9345794392523363No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC10.9345794392523363No Hit
AAGAAATCTCACTCAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGC10.9345794392523363No Hit
GTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCCCCGATGAG10.9345794392523363No Hit
GCATCAGAGAGCACATCCTGGGATCCATTCCGGTGCGAACAAGAGCTCTT10.9345794392523363No Hit
CTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCGGTC10.9345794392523363No Hit
AGATATGGGCCAGCACTAAGCATCAATGAACTGAGCGACCTTGCGAAAGG10.9345794392523363No Hit
GAGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAG10.9345794392523363No Hit
CTTACTATCAGTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACC10.9345794392523363No Hit
TTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGACGAAAAGGCAGCGAGCCC10.9345794392523363No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10.9345794392523363No Hit
ACCTTCCCCTTTTCGATCTTGAAAAATTTGTACGAGGCCAGCCCATCACT10.9345794392523363No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT10.9345794392523363No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT10.9345794392523363No Hit
CATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATG10.9345794392523363No Hit
ATTATATTCCCTATTTGCAATATTAGGCTAATTAGTCCGACTACCAGACA10.9345794392523363No Hit
ACACCGACGTGGTAAACTGTGTGAGCATGGAGTTGTCTCTCACTGACCCA10.9345794392523363No Hit
ATTCCAGATTTGACTCGCTTGCTTGGTCAGCAAGTTCCTGTCATCATGGC10.9345794392523363No Hit
TGCCAGTTGTCTCTGCACACACACATCACTTTGCCGGTATCAGGGTAACA10.9345794392523363No Hit
ATGTATGCTTTCGCACTGAGTAGAGGCTTTGGGTCCGGCATCATCACCTA10.9345794392523363No Hit
GAGCAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATT10.9345794392523363No Hit
AAACTCTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCT10.9345794392523363No Hit
CTCGCGATATGGTTGCCCAGAGTTATTGAGACTCTCTCGCGTCTCCGAGC10.9345794392523363No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA10.9345794392523363No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT10.9345794392523363No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT10.9345794392523363No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT10.9345794392523363No Hit
CCATTCCCCAATTCATTTTAATTTTTGAGGTTCCATTTGTTCTCACATAC10.9345794392523363No Hit
TGAGGTCGCTAGCAAGTTGGATTCAGAATGAGTTTAACAAGGCATGCGAA10.9345794392523363No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG10.9345794392523363No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers