FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_MRTPCR_NC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_MRTPCR_NC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7217
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCAC450.6235277816267146No Hit
AGCGAAAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATC400.5542469170015242No Hit
GATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGTGA300.4156851877511431No Hit
GTCTCCGGTTACTCGGGAGGCGGAAGGGCCGCCGTCGAAGCATTCGAATC290.401829014826105No Hit
AAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGG270.3741166689760288No Hit
CACGTATCTCGATCCGCAAGCGCTGAACGGCACCAACGACCTGCGGCTCG260.3602604960509907No Hit
CCACTACGCCGGCGCGCGTCACGTCGACGTCACGCCGCTGGCTGACGCGC240.3325481502009145No Hit
AGCAAAAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCAC230.3186919772758764No Hit
GCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAA230.3186919772758764No Hit
GCGTTCGACAGTCCGAGCATCAAGTCGAGATTCTGCACGGCCGCACCGGA220.3048358043508383No Hit
GGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGGTCTCCG210.2909796314258002No Hit
GGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTAAAGGT210.2909796314258002No Hit
AGCGAAAGCAGGTGCTCTCCCAGCTGAGCTATGCCCCCATGTACAGGTAT210.2909796314258002No Hit
AGATACGTGATTGCGTGCGCGTCAGCCAGCGGCGTGACGTCGACGTGACG200.2771234585007621No Hit
TCATACAATGGTCGGAACAGAGGGTTGCCAACCCGCGAGGGGGAGCTAAT190.263267285575724No Hit
GGTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCA190.263267285575724No Hit
GGCTAAAGGTGAGCTTTTACGGGGGGCGTGTGCAGGCACCGGCGCGACGC190.263267285575724No Hit
TATCAAGACGGTGCTCTAACCGACTGAGCTACAGACCCCTGAGTCTGTCT180.24941111265068586No Hit
CCCTAAACGATGTCAACTAGTTGTTGGGGATTCATTTCCTTAGTAACGTA180.24941111265068586No Hit
CTCGCACACCGCCCGTTCTTCGTGCCGGCCTACGGTGCCTATCGGCAAGG170.23555493972564778No Hit
CTTCATTGACGAACACGCCGAGCCGCAGGTCGTTGGTGCCGTTCAGCGCT170.23555493972564778No Hit
TGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAGAAGTGGCT170.23555493972564778No Hit
ACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGTTT170.23555493972564778No Hit
CCCTTGACATGGTCGGAATTCCGCTGAGAGGTGGAAGTGCTCGAAAGAGA160.22169876680060968No Hit
ATGTACAGGTATCCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAA160.22169876680060968No Hit
AAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCGGCTA160.22169876680060968No Hit
TTCCAGAGCTTCTCGCAAAGTTGAGCGCTCACGCTTATCGGCTGTAAATT160.22169876680060968No Hit
TGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCACATCA150.20784259387557155No Hit
CTCTAAGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCA150.20784259387557155No Hit
GCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGGTCTCCGGTTAC150.20784259387557155No Hit
GGCCTACGGTGCCTATCGGCAAGGCATCGTGCTCACCGTGCCGATCGAGC150.20784259387557155No Hit
TTTACGGGGGGCGTGTGCAGGCACCGGCGCGACGCGGATCGGCCTGCCGG140.19398642095053348No Hit
GTGTTGCTGTCCGCCGTGTTCGACAATCTCGGCAAAGGGGCGTCCGGTGC140.19398642095053348No Hit
GTCGAACGCAGCGTGAATCTCGCGCAGGCACCGTCAAAGGTCTGCCCGGC140.19398642095053348No Hit
GTCCGAGCATCAAGTCGAGATTCTGCACGGCCGCACCGGACGCCCCTTTG140.19398642095053348No Hit
CCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACC140.19398642095053348No Hit
ATCACGTATCTCGATCCGCAAGCGCTGAACGGCACCAACGACCTGCGGCT140.19398642095053348No Hit
GGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTT130.18013024802549535No Hit
CCTTTGACGGTGCCTGCGCGAGATTCACGCTGCGTTCGACAGTCCGAGCA130.18013024802549535No Hit
CCCCTTTGCCGAGATTGTCGAACACGGCGGACAGCAACACCTGTCCGCGT130.18013024802549535No Hit
CCCGGTGAGCATCCACGCGGTCTCCGGTTACTCGGGAGGCGGAAGGGCCG130.18013024802549535No Hit
TCCACGCGGTCTCCGGTTACTCGGGAGGCGGAAGGGCCGCCGTCGAAGCA120.16627407510045725No Hit
GCTGCCGCGCGACTACCCGGTGAGCATCCACGCGGTCTCCGGTTACTCGG120.16627407510045725No Hit
CCTTAGTAACGTAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTC120.16627407510045725No Hit
GTGCTCACCGTGCCGATCGAGCTGCGCCTGCTTCCGGCCGGCGTGACCGG120.16627407510045725No Hit
TGCGTGTCTCGCGCACCACTACGCCGGCGCGCGTCACGTCGACGTCACGC120.16627407510045725No Hit
TCAATGAAGAACGCGGACAGGTGTTGCTGTCCGCCGTGTTCGACAATCTC120.16627407510045725No Hit
AGCATGTTCCCGAGATCCGGCTGCATGCCGGGCTCGCACACCGCCCGTTC110.15241790217541915No Hit
ACCCGGTGAGCATCCACGCGGTCTCCGGTTACTCGGGAGGCGGAAGGGCC110.15241790217541915No Hit
TCTCCCAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGC110.15241790217541915No Hit
CCTCAGGATTCCAGCCTGTCGGTTTAGGATGCTCGCAAACGCCAAGGCGC110.15241790217541915No Hit
TACCAGAAGTGGCTAGTCTAACCGCAAGGAGGACGGTCACCACGGTAGGA110.15241790217541915No Hit
CTTCCACCTCTCAGCGGAATTCCGACCATGTCAAGGGTAGGTAAGGTTTT110.15241790217541915No Hit
GGTGAGCACGATGCCTTGCCGATAGGCACCGTAGGCCGGCACGAAGAACG110.15241790217541915No Hit
TCCTAAACCGACAGGCTGGAATCCTGAGGATACCTGTACATGGGGGCATA110.15241790217541915No Hit
GCTAAAGGTGAGCTTTTACGGGGGGCGTGTGCAGGCACCGGCGCGACGCG110.15241790217541915No Hit
ACGCGGATCGGCCTGCCGGGCAGACCTTTGACGGTGCCTGCGCGAGATTC110.15241790217541915No Hit
TGCTTGTGCTCCAGCGCCAGGCCGTACACCTGCAGCGGCAGCACGCGTGC110.15241790217541915No Hit
GCGTAGTGGTGCGCGAGACACGCATGCAGCTGCTCACCGGTCACGCCGGC110.15241790217541915No Hit
CCCCTGAGTCTGTCTTTAATTTACAGCCGATAAGCGTGAGCGCTCAACTT100.13856172925038104No Hit
AGCTGCGCCTGCTTCCGGCCGGCGTGACCGGTGAGCAGCTGCATGCGTGT100.13856172925038104No Hit
TCATTGACGAACACGCCGAGCCGCAGGTCGTTGGTGCCGTTCAGCGCTTG100.13856172925038104No Hit
GTGTACGGCCTGGCGCTGGAGCACAAGCATGTTCCCGAGATCCGGCTGCA100.13856172925038104No Hit
TAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGTCGCAAGATTAAA100.13856172925038104No Hit
CCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTTGGGGATTCAT100.13856172925038104No Hit
GCACCAACGACCTGCGGCTCGGCGTGTTCGTCAATGAAGAACGCGGACAG100.13856172925038104No Hit
ATTTACAGCCGATAAGCGTGAGCGCTCAACTTTGCGAGAAGCTCTGGAAA100.13856172925038104No Hit
GTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCACATCAT100.13856172925038104No Hit
GGTCGGAACAGAGGGTTGCCAACCCGCGAGGGGGAGCTAATCCCAGAAAA100.13856172925038104No Hit
GTTACGACTTCACCCCAGTCATGAATCCTACCGTGGTGACCGTCCTCCTT100.13856172925038104No Hit
CCGTAGGCCGGCACGAAGAACGGGCGGTGTGCGAGCCCGGCATGCAGCCG100.13856172925038104No Hit
ATTCTGCACGGCCGCACCGGACGCCCCTTTGCCGAGATTGTCGAACACGG100.13856172925038104No Hit
GTTCCCGAGATCCGGCTGCATGCCGGGCTCGCACACCGCCCGTTCTTCGT100.13856172925038104No Hit
AAGCAGGCCGGCCTGCTGCCGCGCGACTACCCGGTGAGCATCCACGCGGT100.13856172925038104No Hit
AGTCGAGATTCTGCACGGCCGCACCGGACGCCCCTTTGCCGAGATTGTCG90.12470555632534293No Hit
CCCGAGATCCGGCTGCATGCCGGGCTCGCACACCGCCCGTTCTTCGTGCC90.12470555632534293No Hit
CTCCCCAGGCGGTCAACTTCACGCGTTAGCTACGTTACTAAGGAAATGAA90.12470555632534293No Hit
GTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTA90.12470555632534293No Hit
CTGCACGGCCGCACCGGACGCCCCTTTGCCGAGATTGTCGAACACGGCGG90.12470555632534293No Hit
GTGTGCGAGCCCGGCATGCAGCCGGATCTCGGGAACATGCTTGTGCTCCA90.12470555632534293No Hit
GCCGCACCGGACGCCCCTTTGCCGAGATTGTCGAACACGGCGGACAGCAA90.12470555632534293No Hit
GCGAAAGCAGGGATGAATGCCGCGGCGGGCGACGATAGGGCCTGAAATCG90.12470555632534293No Hit
GGCTCGCACACCGCCCGTTCTTCGTGCCGGCCTACGGTGCCTATCGGCAA90.12470555632534293No Hit
AGCGAAAGCAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA90.12470555632534293No Hit
GGCGCAGCTCGATCGGCACGGTGAGCACGATGCCTTGCCGATAGGCACCG90.12470555632534293No Hit
TTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTC90.12470555632534293No Hit
GCAACACCTGTCCGCGTTCTTCATTGACGAACACGCCGAGCCGCAGGTCG90.12470555632534293No Hit
GTCTAACCGCAAGGAGGACGGTCACCACGGTAGGATTCATGACTGGGGTG90.12470555632534293No Hit
ACTACCCGGTGAGCATCCACGCGGTCTCCGGTTACTCGGGAGGCGGAAGG90.12470555632534293No Hit
GGCTGACGCGCACGCAATCACGTATCTCGATCCGCAAGCGCTGAACGGCA90.12470555632534293No Hit
TTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGC90.12470555632534293No Hit
TTCCAGCTTCATGCACTCGAGTTGCAGAGTGCAATCCGGACTACGATCGG90.12470555632534293No Hit
ACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACG90.12470555632534293No Hit
GTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTC90.12470555632534293No Hit
CGCATGCAGCTGCTCACCGGTCACGCCGGCCGGAAGCAGGCGCAGCTCGA90.12470555632534293No Hit
ATTAAAGACAGACTCAGGGGTCTGTAGCTCAGTCGGTTAGAGCACCGTCT90.12470555632534293No Hit
GTTCGACAGTCCGAGCATCAAGTCGAGATTCTGCACGGCCGCACCGGACG90.12470555632534293No Hit
GTGTGTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGAT90.12470555632534293No Hit
ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGCG90.12470555632534293No Hit
GGCGACGATAGGGCCTGAAATCGGCTAAAGGTGAGCTTTTACGGGGGGCG80.11084938340030484No Hit
AATCCACATCATCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTT80.11084938340030484No Hit
GACGGTGCCTGCGCGAGATTCACGCTGCGTTCGACAGTCCGAGCATCAAG80.11084938340030484No Hit
CTGCAACTCGAGTGCATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCA80.11084938340030484No Hit
CGCCGAGCCGCAGGTCGTTGGTGCCGTTCAGCGCTTGCGGATCGAGATAC80.11084938340030484No Hit
GTGCAGGCACCGGCGCGACGCGGATCGGCCTGCCGGGCAGACCTTTGACG80.11084938340030484No Hit
CTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTTTACCAGAAGTGG80.11084938340030484No Hit
GTCGAACACGGCGGACAGCAACACCTGTCCGCGTTCTTCATTGACGAACA80.11084938340030484No Hit
TGCAGCACCTGTGCGCCGGTTCTCTTTCGAGCACTTCCACCTCTCAGCGG80.11084938340030484No Hit
ACGATAGGGCCTGAAATCGGCTAAAGGTGAGCTTTTACGGGGGGCGTGTG80.11084938340030484No Hit
GTCCCGCAACGAGCGCAACCCTTGTCCTTAGTTGCTACGCAAGAGCACTC80.11084938340030484No Hit
GAGTTGCAGAGTGCAATCCGGACTACGATCGGTTTTCTGGGATTAGCTCC80.11084938340030484No Hit
GTTTAGGATGCTCGCAAACGCCAAGGCGCTTACAACCACCAACACGTCAG80.11084938340030484No Hit
GTCCTTAGTTGCTACGCAAGAGCACTCTAAGGAGACTGCCGGTGACAAAC80.11084938340030484No Hit
CTTCCGGCCGGCGTGACCGGTGAGCAGCTGCATGCGTGTCTCGCGCACCA80.11084938340030484No Hit
CTACGCCGGCGCGCGTCACGTCGACGTCACGCCGCTGGCTGACGCGCACG80.11084938340030484No Hit
ATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGT80.11084938340030484No Hit
CCCATGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGC80.11084938340030484No Hit
GTGGTGACCGTCCTCCTTGCGGTTAGACTAGCCACTTCTGGTAAAACCCA80.11084938340030484No Hit
GATAGGGCCTGAAATCGGCTAAAGGTGAGCTTTTACGGGGGGCGTGTGCA80.11084938340030484No Hit
CAGCTGAGCTATGCCCCCATGTACAGGTATCCTCAGGATTCCAGCCTGTC80.11084938340030484No Hit
GACCTTTGACGGTGCCTGCGCGAGATTCACGCTGCGTTCGACAGTCCGAG80.11084938340030484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGCA151.1802962E-4145.05
GCAAAAG151.1802962E-4145.03
AGCAAAA151.1802962E-4145.02
AGCGAAA100.0069463444145.01
CAAAAGC151.1802962E-4145.04
ACCGGAC259.010114E-487.0145
AAAGCAG350.003424431462.1428576
TGAGCTT450.00925793748.3333329
TAAAGGT450.00925793748.3333323
AAGCAGG450.00925793748.3333327
AAAAAAA3306.794926E-77.4696975140-144
Produced by FastQC (version 0.11.5)