FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_Lib_prep_NC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_Lib_prep_NC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTACAGTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAG21.4184397163120568No Hit
GGAACGCAGGACCTTTTTTCTGACCCAAGGTGCCTTACTGAATGACAAGC10.7092198581560284No Hit
TCCCCGAGGTCGGCCGTGCGGTCCTCGGGTGAGGACCCGAGTTTGCAAAG10.7092198581560284No Hit
TCTCCGCACTGATCACCCAGGTGATGTCACTTTTGTCAAGGATATGGCCA10.7092198581560284No Hit
CCTACATCTTTACAGTCGTCAAAGTCTACCTTTTCTGGTGCCATGTTCTC10.7092198581560284No Hit
ATTCTTCCAGGTACAGAAGGCAGTATTCACCGAAACAAACCCAACCTCAG10.7092198581560284No Hit
GAAACAAGGTCGTTTTTAACCAATTCACCCCAAATTGATGGCAATCGGAA10.7092198581560284No Hit
TTTCATATAGGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCAC10.7092198581560284No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC10.7092198581560284No Hit
GAATTGGGGTGTTTATTGCTGCTTCTCTCTACCCTTCATGTCCTTCATGT10.7092198581560284No Hit
TCACATGGAGATGAGGCGTTGCCTCCTCCAGTCACTTCAACAAATTGAGA10.7092198581560284No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC10.7092198581560284No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG10.7092198581560284No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA10.7092198581560284No Hit
ATATGTCCAAATGTCCAGAAATCCATCATCAACTTTTTTATTTAAATTTT10.7092198581560284No Hit
CAGTTATCCCTCTGCAGTTAGAAAAGAAGGCTTCTGTCTCTTATACACAT10.7092198581560284No Hit
CATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGG10.7092198581560284No Hit
GCATTATCCACTGCTCCGTATGCATTTATGTTTACGTAACCATCAAATAT10.7092198581560284No Hit
GGCCTATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCT10.7092198581560284No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA10.7092198581560284No Hit
GTATCTCGCGGGTGCGAGACTGCGACATTAGATTTCTTAGTTCTTTTATT10.7092198581560284No Hit
TAGTAGCACTCCCACAACTAACTCAATGCTTGTGTAAGGAAGGAGTTGAA10.7092198581560284No Hit
GGAATGATAGATGGATGGTATGGTTATCATCATCAGAATGAACAGGGATC10.7092198581560284No Hit
CGGTTGCCTTTGGTGTAGCGTGCCACGTCGAGAACTGTCTCTTATACACA10.7092198581560284No Hit
CGCCTGCTCGAACAGCGCGATGCGCGTCTCGCGCCACAGCTCCTTGCCGA10.7092198581560284No Hit
GCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTAGTGTCGAATA10.7092198581560284No Hit
TTCTTATGCTATTGTCTTTGCTGTATATAGCCCACCCACGGATGGGACAA10.7092198581560284No Hit
GTAGTGCCGACGGCCTGGCAGAAGGCTCGAATGGCGGCTGCTGGGTATCC10.7092198581560284No Hit
CCCCTCCTTCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCT10.7092198581560284No Hit
GGCAATGATCATGCCATCCTTATCGCGCTTGTGCATCGGCACGAGGTCAA10.7092198581560284No Hit
CCATTGGATCAATCTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAA10.7092198581560284No Hit
ATTAATCAGGGGACGACCTAAAGAAAAAACAATCTGGACTAGTGCGAGCA10.7092198581560284No Hit
GGATGGGACAAAGAGATGAATTGCCGGTTAATATCACTGAAGTTGTGTCC10.7092198581560284No Hit
CTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCT10.7092198581560284No Hit
GTCCGAGAGAGGAGAAGAGACAATTGAAGAAAGGTTTGAAATCACAGGAA10.7092198581560284No Hit
GCCCAGCTGAGCCCTTCCCAAGTCCCTGAACTCACAATGTTGTGAGCAAG10.7092198581560284No Hit
GGAAAGGACTGGAATGGAGTGAAATGGAATGGAATGGAATTAACCCGAAT10.7092198581560284No Hit
GAAGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGC10.7092198581560284No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG10.7092198581560284No Hit
GTGTTCTTGAATACAAGTCAAAGAGGAGTACTTGAAGATGAACAAATGTA10.7092198581560284No Hit
TTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATA10.7092198581560284No Hit
GGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAATCTCAA10.7092198581560284No Hit
CTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTC10.7092198581560284No Hit
ATATGTGACAGCAAGAAGAGGTATGTGGTGTGCTGAGTACAAAATATTGG10.7092198581560284No Hit
ACATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGA10.7092198581560284No Hit
TCCTTATCAAGTACAATAGTTGAACATGGCGTGGTAGCTGAAAGTGTAAG10.7092198581560284No Hit
ATGATGTTTTGGTTGCATATTCCAGTATGGTTTTGACTTCCAGTTTGAAT10.7092198581560284No Hit
CCTAACTATAGAGAGAATGGGGCTCTCTGCTTGTGACGAAACGAGAAATA10.7092198581560284No Hit
CATCTTCTCCAATCTCATCGAGCTCTATCCAGCTTGAATCTGTCAGTTCG10.7092198581560284No Hit
CATTACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTC10.7092198581560284No Hit
TCATATAGCCCCCCAAGATCAAAGGTGTTCCTGTGATTTCAAACCTTTCT10.7092198581560284No Hit
GGCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACG10.7092198581560284No Hit
ATGTTCCTGTTACCCTGATACCGGCAAAGTGATGTGTGTGTGCAGAGACA10.7092198581560284No Hit
AGTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATG10.7092198581560284No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC10.7092198581560284No Hit
CAGTAAGGCACCTTAGGTCAGAAAAAAGGTCCTGCATTCCAAGTGAGAAC10.7092198581560284No Hit
TATTAGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGC10.7092198581560284No Hit
GGTTTTGATTGGCTGTGTGTTAACTGCCTCAAATCCATGGCCATAGGGCT10.7092198581560284No Hit
CGGCCCGCGACTAAACATCTATCAGCCACGCAGAATATCCACTCTCCCTA10.7092198581560284No Hit
GGCGCCCGGATCGCCAGCCCCCACTGAACACCACGACAGAACCTCACAAA10.7092198581560284No Hit
GGGGAATGGCGATGAGGCGTTGCCTCCTCCAGTCACTTCAACAAATTGAG10.7092198581560284No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA10.7092198581560284No Hit
AAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTTTGTCATAAG10.7092198581560284No Hit
CCCCACTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGAC10.7092198581560284No Hit
TCTTTCAACAGTGTTTCATAGTTTTCAGTATGAAACACTGTTGAAAGAAA10.7092198581560284No Hit
AGTGGGGCGCTCCTACTGTGATGAATGCATATAAAGTCTGAGCCTCAGAC10.7092198581560284No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT10.7092198581560284No Hit
TGATTGGTGTTTCCTCCACTTCTGGTCCTTATGGCCCAGTACCTGCTTCT10.7092198581560284No Hit
CCTTACATCTATTGCTTGAAGTGGAGCAAGAGATAAGAACTTTCTCGTTT10.7092198581560284No Hit
GCAAACACCGAAACTGGAGCACCGCAACTCAACCCGATTGATGGGCCACT10.7092198581560284No Hit
AAAACAGCTTAACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGAAAGG10.7092198581560284No Hit
GCGGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG10.7092198581560284No Hit
GTCTGAATGTGCCTGTGTAAATGGTTCATGTTTTACTATAATGACTGATG10.7092198581560284No Hit
TCCCAAGGCACCAAACGGTCTTACGAACAGATGGAGACTGATGGAGAACG10.7092198581560284No Hit
GATGGAACAGGCAGCTGTGGTCCAGTGTATGTTGATGGAGCAAACGGAGA10.7092198581560284No Hit
GGCTGGAGTGCTGTGGCATGATTACAGCTTGCTGCAGCCTCAACTTCCTG10.7092198581560284No Hit
GGCTCACACCTATAATCGCAGCACTTTGGGAGGCCGAGGTGGGCAGATCA10.7092198581560284No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATC10.7092198581560284No Hit
ATCTTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCC10.7092198581560284No Hit
TGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATG10.7092198581560284No Hit
AACAGGGCTAGACTGTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGG10.7092198581560284No Hit
CAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCA10.7092198581560284No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCA10.7092198581560284No Hit
CTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGATTGTTTT10.7092198581560284No Hit
GGTTGGGGTGGAAAATTACTAAGGTGAAAAACTTGCCTAGGGAAGCTTTC10.7092198581560284No Hit
CTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAAG10.7092198581560284No Hit
GTTAAACTGTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAA10.7092198581560284No Hit
CACTCCGAATTAGTTTTAAGTGTCTTCTGCCTCCATTAGGAGACCTACAA10.7092198581560284No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG10.7092198581560284No Hit
GATTGGTACAGAAAATGTTCAGGTCTTATTAAACTCCACTAGGACTGTCT10.7092198581560284No Hit
CTTCAAGTCTCTGTGCGTTCTCGGCTTTGAGGGGGCCTGACGGGATGATA10.7092198581560284No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT10.7092198581560284No Hit
CCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAG10.7092198581560284No Hit
TAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGGTCAGA10.7092198581560284No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG10.7092198581560284No Hit
AGTCCAGATTGTTTTTTCTTTAGGTCGTCCCCTGATTAATTCAACCCAGA10.7092198581560284No Hit
ACTTGATCATGCGGTTGTCCGACGGTTACGACACGCTCGTAGGCTGTCTC10.7092198581560284No Hit
GGGCTATATACAGCAAAGACAATAGCAGAAGAATTGGTTCCAAAGGAGAC10.7092198581560284No Hit
GGTCTGGTTCGCCATCAGCTACGGACTGCTCGCGGTGGCCCGGTCGACGG10.7092198581560284No Hit
GTATGATTGAAGCTGAGTCCTCTGTCAAAGAGAAAGACATGACCAAAGAG10.7092198581560284No Hit
GGACAAGAGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTG10.7092198581560284No Hit
CTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAAT10.7092198581560284No Hit
CTTGGAATGCAGGACCTTTTTTCTGACCCAAGGTGCCTTACTGAATGACA10.7092198581560284No Hit
ATCAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGGACAACCTTGAAC10.7092198581560284No Hit
GTCCTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTT10.7092198581560284No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10.7092198581560284No Hit
AATTGATGGTCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT10.7092198581560284No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA10.7092198581560284No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.7092198581560284No Hit
ACACTGTTTTTCTGGAATTTGCAAGTGGAGATTTCAGCCGCTTTGAGGTC10.7092198581560284No Hit
GTTCCTTCCTTCACCATCTGCAAAGGGTCAGAAGGGAAGCTGTCTCTTAT10.7092198581560284No Hit
TTGTTACTCTTATTGTTATTGTTTAATACAGGACATTATTTATCCCCTGA10.7092198581560284No Hit
CAGTTAGGTAACGCGACGCAGGTACAGAGGCCATGGTCATTTTAAGTGCC10.7092198581560284No Hit
TGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTT10.7092198581560284No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTGAGCATC10.7092198581560284No Hit
CAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGGACAACCTTGAACCT10.7092198581560284No Hit
GTGTGGTAGGGAAGGGATTTCATTTACTGGGTAAGGACTGTCTCTTATAC10.7092198581560284No Hit
ACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCTGCAAA10.7092198581560284No Hit
GTCTAGATTTGATATGAAGATATTCCCGTTTCCAACGAAATCTTCAAATC10.7092198581560284No Hit
GTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTTCAAAAGTGCATT10.7092198581560284No Hit
TCTTTCCCTACTGGAAACTGGCAACGCAAGAGCAGATCAGCATTGGGCTG10.7092198581560284No Hit
TACTACCACTGTCCAGCTCCGAATCCGCCTCTGACCATCAGATCAAATGT10.7092198581560284No Hit
AGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCT10.7092198581560284No Hit
ATGAGAACCTCAAGATCAGTGTTCTTCCCTGCAAAGACATCTTCAAGTCT10.7092198581560284No Hit
GATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGC10.7092198581560284No Hit
ACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCAATCT10.7092198581560284No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA10.7092198581560284No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA10.7092198581560284No Hit
GTTCAGCAGCCAGTAAAAAACCTGAAGCCTAATAATCATGTGTGTGACCC10.7092198581560284No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG10.7092198581560284No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG10.7092198581560284No Hit
GACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAC10.7092198581560284No Hit
GAAGGCTAATGTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAAC10.7092198581560284No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA10.7092198581560284No Hit
GTGTGTGCAGAGACAACTGGCATGGTTCGAACCGGCCATGGGTGTCTTTC10.7092198581560284No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG10.7092198581560284No Hit
GGAAGTTGATAGGAAGCTGACAACTATCCATTATCAGAACCTCTTGGGTA10.7092198581560284No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT10.7092198581560284No Hit
GTAAAAGGATTTTCATATAGGTATGGTAATGGTGTTTGGATAGGAAGGAC10.7092198581560284No Hit
CTGTCTGGTAGTCGGACTAATTAGCCTAATATTGCAAATAGGGAATATAA10.7092198581560284No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers