FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_KO_PR8_F1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_KO_PR8_F1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623195
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT311811.1886649677206613No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT195510.745312491065285No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC176040.6710900257129189No Hit
TTTCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATA170090.6484077622898793No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT166880.6361707764767773No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA133800.5100650161349042No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA123920.47240102241731935No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG101880.3883813441242454No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT81780.31175722735061634No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA76850.2929633519429551No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA75630.2883125349049537No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT70260.26784131564752145No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG69640.2654777856773896No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT68560.2613606689552244No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA62590.23860216262992268No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTACTTTGCCGGTATCAGG60430.23036792918559237No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG58980.22484031877157434No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT56430.21511934873312885No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA55140.2102016815372094No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC54790.20886743074761882No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG54460.20760942286029058No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA53500.20394976355169936No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG52320.19945143231822263No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA51780.19739287395714006No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA50410.1921702351521713No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC48110.18340230139200478No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC46310.1765404401883962No Hit
CTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG43280.1649896404956551No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG42860.16338853954814644No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGT41900.1597288802395552No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA41550.15839462944996463No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT40860.15576424932191468No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG40810.1555736420662589No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC40740.1553067919083408No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT40200.15324823354725822No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC39880.15202834711106114No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG39480.1505034890658148No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG39110.1490929953739619No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG39080.14897863102056844No Hit
CTTTAATTCGGGCTCTGGAAACCATAGCCTCCACCATACTGGATATCCCG38080.14516648590745257No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT37960.14470902849387865No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT36460.13899081082420484No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA36130.1377328029368766No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA35960.1370847382676469No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA35770.1363604306961549No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA35140.1339587792748919No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG34090.1299560269061202No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT33630.1282024401540869No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA33590.12804995434956226No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG33460.1275543754848572No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG33310.12698255371788983No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT33250.12675382501110288No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT33190.1265250963043159No Hit
GTACTGATGTGTCCTGTTGACAGTATCCATGGTGTATCCTGTTCCTGTCC33170.12644885340205358No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT32290.12309416570251164No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA32050.12217925087536381No Hit
GGATACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTC31020.11825274140885447No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA30830.11752843383736244No Hit
GGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG29860.11383065307764004No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA29670.11310634550614804No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG29660.11306822405501687No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA28200.1075024921898677No Hit
CGTTCCTGTTACCCTGATACCGGCAAAGTAAATCGATCCATCTTGAATAC28080.10704503477629379No Hit
GTATTCAAGATGGATCGATTTACTTTGCCGGTATCAGGGTAACAGGAACG28040.10689254897176916No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG27030.10304228240752213No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC26900.10254670354281706No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT26790.10212736758037431No Hit
GTCATCGTTCAGGCTCTTAGGGACAACCTTGAACCTGGGACCTTTGATCT26380.10056438808399681No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG26360.10048814518173449No Hit
ATTCAAGATGGATCGATTTACTTTGCCGGTATCAGGGTAACAGGAACGTT26340.10041190227947218No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA26250.10006880921929173No Hit
TTTCTTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACTGGAG26250.10006880921929173No Hit
ATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATCTTCATGATCTCA26240.10003068776816058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16650.0132.479161
GCAAAAG104950.0106.124133
AGCAGGC57750.0104.101948
CAAAAGC111900.099.857084
AGTAGAA36900.097.924461
GCAGGCA62200.096.5375449
GAGCAAA119850.093.535971
AAGCAGG147100.090.855747
GCAGGGT7050.089.492459
AAAAGCA125450.089.360595
AAAGCAG164650.081.259546
ATTAGTA3950.077.1507951
GCAGGGG17000.076.3590249
GCAGGTC20300.075.0204249
AGCGAAA29800.073.5324252
AGCAGGG26600.072.792378
TATTAGT1500.072.558491
TTACGCC502.3090415E-672.490715145
GTAGAAA52050.069.561352
TAAACTA16300.067.216765
Produced by FastQC (version 0.11.5)