FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_KO_PR8_F1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_KO_PR8_F1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2982302
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT351081.1772114292918692No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT226860.7606875494165245No Hit
TTTCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATA190230.6378629662589502No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC174730.5858896919225485No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA141260.4736609505006535No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA130160.4364413798468431No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT118220.39640519303544713No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT110970.3720951131039043No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG105430.3535188589217323No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA99740.33443963756856276No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT75710.25386429677477335No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT73720.247191598972874No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA72270.24232958298656543No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTACTTTGCCGGTATCAGG67080.2249269188700541No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG64610.21664472612096292No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA63010.21127974296365695No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT60710.2035675796750296No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA58400.195821885241669No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG57950.1943129837286767No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG57240.19193227245262218No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG56870.19069162009749516No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA56550.18961862346603395No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA54420.18247648963787033No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG52460.1759043852701705No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC51640.17315483140205118No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC51500.1726853953757869No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT50700.1700029037971339No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC50200.16832634656047576No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA48790.16359845515309984No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA46440.15571863614080667No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC41860.14036137185301822No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA41220.13821537859009583No Hit
CTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG39760.13331983145905413No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT39760.13331983145905413No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG39590.13274980199859035No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG39510.13248155284072505No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG39040.1309055890382664No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG38870.13033555957780266No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGT38620.12949728095947358No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT38090.12772013028861598No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA37820.12681478938082058No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG37040.1241993600916339No Hit
CTTTAATTCGGGCTCTGGAAACCATAGCCTCCACCATACTGGATATCCCG36840.12352873719697066No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA36830.1234952060522375No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA36700.12305930117070638No Hit
GTACTGATGTGTCCTGTTGACAGTATCCATGGTGTATCCTGTTCCTGTCC36360.12191924224977888No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT35830.12014209157892124No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC35820.12010856043418809No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA35750.11987384242105595No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA34690.11631954107934073No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA34670.1162524787898744No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA34560.1158836361978096No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG34550.11585010505307644No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA33660.112865833171825No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT33480.11226227256662805No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG33040.11078690219836893No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG32340.10843972206704754No Hit
GGATACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTC32180.10790322375131695No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA31630.106059010790993No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT31520.10569016819892822No Hit
CGTTCCTGTTACCCTGATACCGGCAAAGTAAATCGATCCATCTTGAATAC31400.10528779446213027No Hit
GGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG31150.10444951584380119No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG30830.10337651921233999No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA30750.1031082700544747No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT30740.10307473890974153No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG30610.10263883402821042No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC30570.10250470944927778No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA30380.10186761769934768No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG30220.1013311193836171No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA30110.1009622767915523No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT29850.10009046702849006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA19750.0132.23271
GCAAAAG130150.0107.799263
AGCAGGC62000.0104.7943048
CAAAAGC142150.098.801144
GAGCAAA148850.094.890031
GCAGGCA69500.093.3812269
AAGCAGG181550.091.426247
AAAAGCA161850.086.77535
GCAGGGG28300.084.813099
AAAGCAG203500.081.707326
GCAGGGT13350.079.846779
AGTAGAA36550.079.788841
AGCGAAA33300.078.208452
AGCAGGG44400.077.903468
GCAGGTC25550.077.480629
ATTAGTA4050.073.439941
GCTGAGC300.001937351272.48838145
TGACGCC5050.068.89985145
TCTCGGG7200.068.46125145
ACGGCCC754.8657967E-967.65582145
Produced by FastQC (version 0.11.5)