FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_KO_PBS_F1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_KO_PBS_F1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399090
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG54780.39154021542574097No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG37750.26981823899820595No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA26740.1911242307499875No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC26300.1879793294212667No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG20660.14766741238948175No Hit
GAGCAAAAGCAGGCTGCACCACGCTGCACAGCGGCAAAGGATGGTGATGA18410.13158553059488667No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT16710.11943477546119262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA64350.0104.393531
AGCAAAA69300.095.9947052
GAGCGAA15800.095.0439451
GCAAAAG76050.088.23763
CAAAAGC80050.083.6472244
AAAAGCA96950.069.290615
AAAGCAG131950.062.75986
AGCGAAA24250.062.2246932
GCGAAAG24800.061.1372383
GCAGGCC19500.060.2428139
AAGCAGG138500.059.894317
AGCAGGC48300.059.002488
CGAAAGC25950.058.707444
TGACGCC2850.055.9581145
AGCAGGG80850.043.2306568
AGCAGGT24600.042.7422838
GCTCGGA907.456276E-540.3031231
GCAGGTG16100.036.4824459
GGAGCGA3450.035.7471161
TCGCCGG5350.033.87438145