Basic Statistics
Measure | Value |
---|---|
Filename | HT5CKBCX2_l02_n02_KO_PBS_F1_rep1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1399090 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG | 5478 | 0.39154021542574097 | No Hit |
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG | 3775 | 0.26981823899820595 | No Hit |
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA | 2674 | 0.1911242307499875 | No Hit |
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC | 2630 | 0.1879793294212667 | No Hit |
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG | 2066 | 0.14766741238948175 | No Hit |
GAGCAAAAGCAGGCTGCACCACGCTGCACAGCGGCAAAGGATGGTGATGA | 1841 | 0.13158553059488667 | No Hit |
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT | 1671 | 0.11943477546119262 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAGCAAA | 6435 | 0.0 | 104.39353 | 1 |
AGCAAAA | 6930 | 0.0 | 95.994705 | 2 |
GAGCGAA | 1580 | 0.0 | 95.043945 | 1 |
GCAAAAG | 7605 | 0.0 | 88.2376 | 3 |
CAAAAGC | 8005 | 0.0 | 83.647224 | 4 |
AAAAGCA | 9695 | 0.0 | 69.29061 | 5 |
AAAGCAG | 13195 | 0.0 | 62.7598 | 6 |
AGCGAAA | 2425 | 0.0 | 62.224693 | 2 |
GCGAAAG | 2480 | 0.0 | 61.137238 | 3 |
GCAGGCC | 1950 | 0.0 | 60.242813 | 9 |
AAGCAGG | 13850 | 0.0 | 59.89431 | 7 |
AGCAGGC | 4830 | 0.0 | 59.00248 | 8 |
CGAAAGC | 2595 | 0.0 | 58.70744 | 4 |
TGACGCC | 285 | 0.0 | 55.9581 | 145 |
AGCAGGG | 8085 | 0.0 | 43.230656 | 8 |
AGCAGGT | 2460 | 0.0 | 42.742283 | 8 |
GCTCGGA | 90 | 7.456276E-5 | 40.303123 | 1 |
GCAGGTG | 1610 | 0.0 | 36.482445 | 9 |
GGAGCGA | 345 | 0.0 | 35.747116 | 1 |
TCGCCGG | 535 | 0.0 | 33.87438 | 145 |