FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_KO_Control_M_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_KO_Control_M_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045085
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG163720.8005535222252376No Hit
GAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTA82590.4038462948972781No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG67730.3311842784040761No Hit
AAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATG57930.2832645097881017No Hit
GAGCAAAAGCAGGGGTACCCTTGTAAGAAATTTTCACCACAGAAGGAGCC46910.2293792189566693No Hit
GGTGAGTGCAAAGGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCT46030.22507621932584707No Hit
GCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGA38750.18947867692540898No Hit
GGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAGCTACAGTAATGGGC36670.1793079505252838No Hit
GAGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGG35670.1744181782175313No Hit
GTAATGGGCCCTGCTCCTTTGTGAGACAAAGGTGAGTGCAAAGGGTTGCA34830.17031076947901921No Hit
AGTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGA34130.16688792886359247No Hit
GAGATAAGGAGGGAGGATGGGAAAGAAAGGGAGGGGAGGAGAGGTCAAGG31000.15158294154032717No Hit
GTAGAAACAAGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGAC29890.1461552942787219No Hit
AGCGAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG29670.14507954437101636No Hit
AGCAAAAGCAGGGAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGG29530.14439497624793102No Hit
ATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGGCAGAGAGAGAGAGG29410.14380820357100071No Hit
GGGTTGCAACTGAGCTGCCTTCTGTCATTTATCTAGCTTTGCCTTCCCTC29210.14283024910945022No Hit
GTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAAAG28860.14111882880173685No Hit
AGGTGGAGTAGGGGCTGGTGGGAGAAGTGTGTGATGTGGACCATGGTAAA28140.13759819274015506No Hit
TCCTTGACCTCTCCTCCCCTCCCTTTCTTTCCCATCCTCCCTCCTTATCT27840.1361312610478293No Hit
GTATAATAACAATCTGAACGGAATCTTAGCTGATGAAATGGGGCTAGGCA27690.13539779520166645No Hit
GTTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCT26500.12957896615544096No Hit
CCTTATCTCCCTCCATGTGTCTTCCCCTCCCTCACTCTCAGCCCCATCTT25260.12351564849382789No Hit
GAGCAAAAGCAGGAGGGATCAAGTAAGAGGGAGATGGAGGGAGAAATTAT22440.10972649058596587No Hit
GAGCAAAAGCAGGCAAAAGTGTCAGAGACAGCCTCTGCTCCCGCTGTTAG22030.10772168393978734No Hit
CCCTCACTCTCAGCCCCATCTTCCCTCCCTTTCTCTGCCTCCACACTTCC21620.10571687729360882No Hit
GAGACAGGGAGGGAATCTGGGACTGAGAGGGGGAGGGGAATGTTTGGAGG21550.10537459323206615No Hit
GAGCGAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG21520.10522790006283356No Hit
TTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCTT21280.10405435470897298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA106300.0115.888351
GAGCGAA27000.0111.2428051
AGCAAAA116350.0104.94292
CAAAAGC130700.093.0322954
GCAAAAG136850.089.434613
CGAATAA3900.089.21765145
AAAAGCA147950.082.136315
GACGAAA7350.071.99621145
AGCGAAA44700.066.706732
AAAGCAG234750.064.533856
GCGAAAG47050.063.2207343
AAGCAGG242700.062.7785037
TGACGCC3300.061.506107145
CGAAAGC48450.061.2441864
GCAGGCC33450.059.4031039
AGCAGGC82950.055.5152138
CCTATCG400.00604535854.389618
GCTCGGA553.2089348E-452.763382
AGCAGGG158200.048.499128
AGTAGAA20150.045.3660351
Produced by FastQC (version 0.11.5)