FastQCFastQC Report
Sun 10 Feb 2019
HT5CKBCX2_l02_n02_DI244G4_P3CW_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5CKBCX2_l02_n02_DI244G4_P3CW_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3116767
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG199430.6398617541831007No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA130340.41818974597716163No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT118810.38119628448324816No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG111520.35780666312239573No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA109320.3507480668269396No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC103500.3320748711725965No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC98360.31558342346412166No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA96960.31109158945792226No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA91110.292322140217732No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT86950.27897497631359675No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA86260.27676114383911277No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT82980.26623741845315996No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG81720.26219476784758056No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC80990.2598525972586337No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT73100.2345379041808387No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG71150.22828142110077526No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT69840.22407834785211728No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG68850.220901979519162No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT64970.20845318241626662No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC64290.20627143447039833No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC62910.20184376952143038No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT62390.20017537403341346No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC62160.19943742987525215No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA58310.18708488635820386No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT57940.1858977587994226No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT56820.1823042915944631No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA56020.1797375293052063No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA55150.17694617531563958No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT53240.170818030350039No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC52630.1688608741044807No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT51560.16542782954259977No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA51290.1645615472699756No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT51040.16375943405458282No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT51010.1636631804687357No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG50010.16045472760716473No Hit
TCTTATTTCTTCAAACTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTC49730.15955636080592486No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA49500.15881841664776353No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT48930.15698959851666805No Hit
GTTTTGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCG48740.15637999247296958No Hit
GAATGGACCAGGCGATCATGGATAAGAACATCATACTGAAAGCGAACTTC48050.1541661599984856No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA47660.15291486338247293No Hit
CTTTGTATCAGAATGGACCAGGCGATCATGGATAAGAACATCATACTGAA47180.15137480600891884No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA47040.15092562260829892No Hit
GATAAGAACATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCT46600.1495139033492077No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC46320.1486155365479678No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT45520.14604877425871104No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA45300.1453429146291654No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT45110.14473330858546693No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA43940.1409794187374289No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA43750.1403698126937304No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT42260.13558921792998962No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC42250.13555713340137393No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC42020.1348191892432126No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC41760.13398499149920415No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA41660.13366414621304704No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC40860.13109738392379028No Hit
CTCCAAAACAGAAACGAGAAATGGCGGGAACAATTAGGTCAGAAGTTTGA40840.13103321486655886No Hit
CTGCTGAGGATGTCAAAAATGCAGTTGGAGTCCTCATCGGGGGACTTGAA40260.12917231220684766No Hit
GTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTGCCA39600.12705473331821082No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA39550.1268943106751323No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA39350.12625262010281807No Hit
ACCTAATTGTTCCCGCCATTTCTCGTTTCTGTTTTGGAGTGAGTGGAGGT39280.12602802840250812No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA38860.1246804782006483No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGAACT37940.121728701568003No Hit
GTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCGCAAACGAGT37560.12050948948060602No Hit
GGTCCATTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGC37530.1204132358947589No Hit
GGTACAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAG36780.11800689624858066No Hit
ATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT36630.11752562831934502No Hit
CTGTACCTGCGTCGCGTTACCTAACTGACATGACTCTTGAGGAAATGTCA36470.11701227586149365No Hit
CTTCTGACCTAATTGTTCCCGCCATTTCTCGTTTCTGTTTTGGAGTGAGT36420.1168518532184151No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC36310.1164989234036423No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT35970.11540804943070816No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC35870.11508720414455106No Hit
CTTAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACAT35860.11505511961593536No Hit
GCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATTTCACCAT35250.11309796337037704No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT35150.11277711808421996No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT34240.10985742598019037No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG33770.10834945313525202No Hit
GTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCTTC33350.10700190293339219No Hit
CCCTAAGAGGAAGGGGCAGCACCCTCGGTCTGGACATCGAGACAGCCACA33080.10613562066076802No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT32860.10542976103122242No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG32840.10536559197399101No Hit
CTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTATTTCT31990.10263840704165567No Hit
CTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAAT31940.10247798439857712No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA31700.10170795571180008No Hit
GTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGAA31180.10003956022378317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8700.0136.770451
AGCGAAA28450.097.165121
AGCAAAA48400.095.340952
GCAAAAG49250.093.400833
ACTAATA3650.069.538738
ATTAGTA3800.066.827031
AGGTGTT3000.065.2388145
AAGGGTG26800.064.703955
TCTCGGG9750.063.937737145
AGGGTGT27350.063.368186
GCAGGGT42150.062.97029
TAAACTA50350.062.8282175
ACAAGGG31250.062.6875763
CAAGGGT31050.062.6240124
TTTTTAA50500.062.4979251
CTGAGCG350.003569638862.13219145
TTTAAAC51000.062.0274663
TTTTAAA51000.061.88522
GAAACAA47850.061.7134974
TACTAAT4400.060.978927
Produced by FastQC (version 0.11.5)